Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis
Background and Aim: Mastitis is a common and economically important disease in dairy cattle. It remains one of the most common reasons for the extensive use of antimicrobials in dairy farms leading to the emergence of antimicrobial-resistant pathogens. The aim of this study was to determine the patt...
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| Format: | Article |
| Language: | English |
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Veterinary World
2020-08-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.13/August-2020/14.pdf |
| _version_ | 1818643664476307456 |
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| author | Zuhair Bani Ismail Sameeh M. Abutarbush |
| author_facet | Zuhair Bani Ismail Sameeh M. Abutarbush |
| author_sort | Zuhair Bani Ismail |
| collection | DOAJ |
| description | Background and Aim: Mastitis is a common and economically important disease in dairy cattle. It remains one of the most common reasons for the extensive use of antimicrobials in dairy farms leading to the emergence of antimicrobial-resistant pathogens. The aim of this study was to determine the patterns of antimicrobial resistance of Escherichia coli isolates from bovine mastitis and to identify prominent antimicrobial resistance and virulence genes among isolated strains.
Materials and Methods: Antimicrobial susceptibility testing against six antibiotic groups, including tetracyclines, aminoglycosides, beta-lactams, macrolides, sulfonamides, and fluoroquinolones was performed using the disk diffusion method. PCR was performed on resistant isolates to detect resistance and virulence genes using commercially available primers.
Results: Out of 216 milk samples cultured, 14 samples yielded E. coli isolates. All isolates (100%) were resistant to ampicillin, amoxicillin, procaine penicillin, streptomycin, oxytetracycline, and sulfamethoxazole-trimethoprim. Only one isolate (7%) was sensitive to gentamicin, and all isolates (100%) were sensitive to enrofloxacin and ciprofloxacin. All isolates carried at least one resistance gene against one or more of the major antibiotic groups. All isolates carried the ereA, tetG, tetE, and tetB genes, followed by tetA (93%), ampC (86%), strA (86%), sul1 (78%), tetD (71%), tetC (57%), aadA (57%), and strB (36%). The lowest percentage of isolates carried bla1 (17%) and bla2 (12%) genes, and none of the isolates carried the qnrA gene. Most of the isolates (93%) carried the Shiga toxin 1 virulence gene, followed by complement resistance protein (79%), intimin (64%), Shiga toxin 2 (36%), cytotoxic necrotizing factor (35%), aerotaxis receptor (21%), and type 1 fimbriae (15%).
Conclusion: Results of this study indicate that the high percentages of E. coli isolate from bovine mastitis are resistant to two or more of the major antibiotic groups, irrespective of the presence or absence of relevant resistance or virulence genes. |
| first_indexed | 2024-12-17T00:02:33Z |
| format | Article |
| id | doaj.art-144dc873164e4c7e9667b644ce52c06d |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T00:02:33Z |
| publishDate | 2020-08-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-144dc873164e4c7e9667b644ce52c06d2022-12-21T22:11:02ZengVeterinary WorldVeterinary World0972-89882231-09162020-08-011381588159310.14202/vetworld.2020.1588-1593Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitisZuhair Bani Ismail0https://orcid.org/0000-0002-7267-8270Sameeh M. Abutarbush1https://orcid.org/0000-0001-5663-9717Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.Background and Aim: Mastitis is a common and economically important disease in dairy cattle. It remains one of the most common reasons for the extensive use of antimicrobials in dairy farms leading to the emergence of antimicrobial-resistant pathogens. The aim of this study was to determine the patterns of antimicrobial resistance of Escherichia coli isolates from bovine mastitis and to identify prominent antimicrobial resistance and virulence genes among isolated strains. Materials and Methods: Antimicrobial susceptibility testing against six antibiotic groups, including tetracyclines, aminoglycosides, beta-lactams, macrolides, sulfonamides, and fluoroquinolones was performed using the disk diffusion method. PCR was performed on resistant isolates to detect resistance and virulence genes using commercially available primers. Results: Out of 216 milk samples cultured, 14 samples yielded E. coli isolates. All isolates (100%) were resistant to ampicillin, amoxicillin, procaine penicillin, streptomycin, oxytetracycline, and sulfamethoxazole-trimethoprim. Only one isolate (7%) was sensitive to gentamicin, and all isolates (100%) were sensitive to enrofloxacin and ciprofloxacin. All isolates carried at least one resistance gene against one or more of the major antibiotic groups. All isolates carried the ereA, tetG, tetE, and tetB genes, followed by tetA (93%), ampC (86%), strA (86%), sul1 (78%), tetD (71%), tetC (57%), aadA (57%), and strB (36%). The lowest percentage of isolates carried bla1 (17%) and bla2 (12%) genes, and none of the isolates carried the qnrA gene. Most of the isolates (93%) carried the Shiga toxin 1 virulence gene, followed by complement resistance protein (79%), intimin (64%), Shiga toxin 2 (36%), cytotoxic necrotizing factor (35%), aerotaxis receptor (21%), and type 1 fimbriae (15%). Conclusion: Results of this study indicate that the high percentages of E. coli isolate from bovine mastitis are resistant to two or more of the major antibiotic groups, irrespective of the presence or absence of relevant resistance or virulence genes.http://www.veterinaryworld.org/Vol.13/August-2020/14.pdfantimicrobial resistancedairy cowsenvironmental mastitis pathogensescherichia coli |
| spellingShingle | Zuhair Bani Ismail Sameeh M. Abutarbush Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis Veterinary World antimicrobial resistance dairy cows environmental mastitis pathogens escherichia coli |
| title | Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis |
| title_full | Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis |
| title_fullStr | Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis |
| title_full_unstemmed | Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis |
| title_short | Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis |
| title_sort | molecular characterization of antimicrobial resistance and virulence genes of escherichia coli isolates from bovine mastitis |
| topic | antimicrobial resistance dairy cows environmental mastitis pathogens escherichia coli |
| url | http://www.veterinaryworld.org/Vol.13/August-2020/14.pdf |
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