<i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast
Tolerance to harsh environmental conditions, high growth rates and an amino acid profile adequate for human consumption are beneficial features observed in <i>Dunaliella viridis</i> TAV01, a novel strain isolated from a salt pond in the Algarve, Portugal. TAV01 was identified down to the...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/13/4/2146 |
| _version_ | 1797622602349412352 |
|---|---|
| author | Gabriel Bombo Nathana L. Cristofoli Tamára F. Santos Lisa Schüler Inês B. Maia Hugo Pereira Luísa Barreira João Varela |
| author_facet | Gabriel Bombo Nathana L. Cristofoli Tamára F. Santos Lisa Schüler Inês B. Maia Hugo Pereira Luísa Barreira João Varela |
| author_sort | Gabriel Bombo |
| collection | DOAJ |
| description | Tolerance to harsh environmental conditions, high growth rates and an amino acid profile adequate for human consumption are beneficial features observed in <i>Dunaliella viridis</i> TAV01, a novel strain isolated from a salt pond in the Algarve, Portugal. TAV01 was identified down to the species level by maximum likelihood and Bayesian phylogenetic analyses of the ribosomal internal transcribed spacers one and two regions (ITS1 and ITS-2) and was supported by ITS2 secondary structure analysis. The biochemical profile revealed high protein (35.7 g 100 g<sup>−1</sup> DW; 65% higher than the minimum recommended by the World Health Organization) and lipid contents (21.3 g 100 g<sup>−1</sup> DW), a relatively higher proportion of the polyunsaturated fatty acids (PUFAs), α-linolenic (26.3% of total fatty acids (TFA)) and linoleic acids (22.8% of TFA), compared to those of other <i>Dunaliella</i> strains, and a balanced essential amino acids profile containing significant levels of leucine, phenylalanine, valine, and threonine. The major carotenoid was lutein, making up over 85% of total carotenoids. The presence of high-quality natural products in <i>D. viridis</i> TAV01 offers the possibility of using this new strain as a valuable biological resource for novel feed or food products as ingredients or supplements. |
| first_indexed | 2024-03-11T09:12:38Z |
| format | Article |
| id | doaj.art-144f3e3174a94ac697652ce87eade8b0 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T09:12:38Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-144f3e3174a94ac697652ce87eade8b02023-11-16T18:51:33ZengMDPI AGApplied Sciences2076-34172023-02-01134214610.3390/app13042146<i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve CoastGabriel Bombo0Nathana L. Cristofoli1Tamára F. Santos2Lisa Schüler3Inês B. Maia4Hugo Pereira5Luísa Barreira6João Varela7Centre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalGreenCoLab—Associação Oceano Verde, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus Gambelas, 8005-139 Faro, PortugalTolerance to harsh environmental conditions, high growth rates and an amino acid profile adequate for human consumption are beneficial features observed in <i>Dunaliella viridis</i> TAV01, a novel strain isolated from a salt pond in the Algarve, Portugal. TAV01 was identified down to the species level by maximum likelihood and Bayesian phylogenetic analyses of the ribosomal internal transcribed spacers one and two regions (ITS1 and ITS-2) and was supported by ITS2 secondary structure analysis. The biochemical profile revealed high protein (35.7 g 100 g<sup>−1</sup> DW; 65% higher than the minimum recommended by the World Health Organization) and lipid contents (21.3 g 100 g<sup>−1</sup> DW), a relatively higher proportion of the polyunsaturated fatty acids (PUFAs), α-linolenic (26.3% of total fatty acids (TFA)) and linoleic acids (22.8% of TFA), compared to those of other <i>Dunaliella</i> strains, and a balanced essential amino acids profile containing significant levels of leucine, phenylalanine, valine, and threonine. The major carotenoid was lutein, making up over 85% of total carotenoids. The presence of high-quality natural products in <i>D. viridis</i> TAV01 offers the possibility of using this new strain as a valuable biological resource for novel feed or food products as ingredients or supplements.https://www.mdpi.com/2076-3417/13/4/2146biotechnologynatural productspigmentsphylogenyamino acids |
| spellingShingle | Gabriel Bombo Nathana L. Cristofoli Tamára F. Santos Lisa Schüler Inês B. Maia Hugo Pereira Luísa Barreira João Varela <i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast Applied Sciences biotechnology natural products pigments phylogeny amino acids |
| title | <i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast |
| title_full | <i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast |
| title_fullStr | <i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast |
| title_full_unstemmed | <i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast |
| title_short | <i>Dunaliella viridis</i> TAV01: A Halotolerant, Protein-Rich Microalga from the Algarve Coast |
| title_sort | i dunaliella viridis i tav01 a halotolerant protein rich microalga from the algarve coast |
| topic | biotechnology natural products pigments phylogeny amino acids |
| url | https://www.mdpi.com/2076-3417/13/4/2146 |
| work_keys_str_mv | AT gabrielbombo idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT nathanalcristofoli idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT tamarafsantos idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT lisaschuler idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT inesbmaia idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT hugopereira idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT luisabarreira idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast AT joaovarela idunaliellaviridisitav01ahalotolerantproteinrichmicroalgafromthealgarvecoast |