Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential
A total of 20 Lactobacillus strains isolated from fermented dates were tested for their probiotic potential by comparing their pH stability, resistance to low pH, and ability to tolerate bile salts. Out of 20 strains, 3 strains named as <i>Lactobacillus pentosus</i> KAU001, <i>Lact...
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MDPI AG
2022-05-01
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| author | Abdullah Al Malki So-Hyun Yoon Ahmad Firoz Hani Mohammed Ali Yong-Ha Park Yan-Yan Hor Irfan A. Rather |
| author_facet | Abdullah Al Malki So-Hyun Yoon Ahmad Firoz Hani Mohammed Ali Yong-Ha Park Yan-Yan Hor Irfan A. Rather |
| author_sort | Abdullah Al Malki |
| collection | DOAJ |
| description | A total of 20 Lactobacillus strains isolated from fermented dates were tested for their probiotic potential by comparing their pH stability, resistance to low pH, and ability to tolerate bile salts. Out of 20 strains, 3 strains named as <i>Lactobacillus pentosus</i> KAU001, <i>Lactiplantibacillus pentosus</i> KAU002, and <i>Lactiplantibacillus plantarum</i> KAU003 had a high tolerance of acids and bile salts and the capability to adhere to the intestinal wall. In addition, the three isolates were tested for their anti-oxidation, anti-glucosidase inhibition, cholesterol-lowering, and anti-inflammation properties. Among them, strain KAU001 and KAU002 inhibited α-glucosidase, lowered cholesterol level, inhibited nitric oxide production, and showed a higher anti-oxidative ability that was significantly better than strain KAU003. Both strains also significantly inhibited the release of inflammatory mediators such as TNF-α, IL-6 and IL-10 induced by LPS on RAW 264.7 macrophages (<i>p</i> < 0.001). The results indicated that KAU001 and KAU002 have the highest probiotic potential, potentially modulating metabolic health and reducing pro-inflammatory cytokines in response to allergic reactions. |
| first_indexed | 2024-03-10T03:23:04Z |
| format | Article |
| id | doaj.art-14507bad00ab48fdac69f5aaaf5f611e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:23:04Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-14507bad00ab48fdac69f5aaaf5f611e2023-11-23T09:57:29ZengMDPI AGApplied Sciences2076-34172022-05-011210508210.3390/app12105082Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory PotentialAbdullah Al Malki0So-Hyun Yoon1Ahmad Firoz2Hani Mohammed Ali3Yong-Ha Park4Yan-Yan Hor5Irfan A. Rather6Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biotechnology, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, Gyeongbuk-do, KoreaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biotechnology, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, Gyeongbuk-do, KoreaDepartment of Biotechnology, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, Gyeongbuk-do, KoreaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaA total of 20 Lactobacillus strains isolated from fermented dates were tested for their probiotic potential by comparing their pH stability, resistance to low pH, and ability to tolerate bile salts. Out of 20 strains, 3 strains named as <i>Lactobacillus pentosus</i> KAU001, <i>Lactiplantibacillus pentosus</i> KAU002, and <i>Lactiplantibacillus plantarum</i> KAU003 had a high tolerance of acids and bile salts and the capability to adhere to the intestinal wall. In addition, the three isolates were tested for their anti-oxidation, anti-glucosidase inhibition, cholesterol-lowering, and anti-inflammation properties. Among them, strain KAU001 and KAU002 inhibited α-glucosidase, lowered cholesterol level, inhibited nitric oxide production, and showed a higher anti-oxidative ability that was significantly better than strain KAU003. Both strains also significantly inhibited the release of inflammatory mediators such as TNF-α, IL-6 and IL-10 induced by LPS on RAW 264.7 macrophages (<i>p</i> < 0.001). The results indicated that KAU001 and KAU002 have the highest probiotic potential, potentially modulating metabolic health and reducing pro-inflammatory cytokines in response to allergic reactions.https://www.mdpi.com/2076-3417/12/10/5082AjwaMadinahprobioticsinflammationfoods |
| spellingShingle | Abdullah Al Malki So-Hyun Yoon Ahmad Firoz Hani Mohammed Ali Yong-Ha Park Yan-Yan Hor Irfan A. Rather Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential Applied Sciences Ajwa Madinah probiotics inflammation foods |
| title | Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential |
| title_full | Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential |
| title_fullStr | Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential |
| title_full_unstemmed | Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential |
| title_short | Characterization of New Probiotic Isolates from Fermented Ajwa Dates of Madinah and Their Anti-Inflammatory Potential |
| title_sort | characterization of new probiotic isolates from fermented ajwa dates of madinah and their anti inflammatory potential |
| topic | Ajwa Madinah probiotics inflammation foods |
| url | https://www.mdpi.com/2076-3417/12/10/5082 |
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