Micronucleus Assay in Urothelial Cells in Cancer Cervix
Introduction: Cancer ranks third among the ten leading global causes of death. To evaluate the genotoxic risks, observed as DNA damages, can be assessed by Micronucleus (MN) test. Aim: To identify the occurrence of MN in normal and cancer cervix and find the correlation between MN and stage of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9488/23425_CE[Ra1]_F(RK)_PF1(SYGH)_PFA(GH)_PF2(NE_SY_DK).pdf |
| _version_ | 1818531400394997760 |
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| author | Suresh Kumar Sundararajan Pratheepa Sivasankari Natarajan Kanchana |
| author_facet | Suresh Kumar Sundararajan Pratheepa Sivasankari Natarajan Kanchana |
| author_sort | Suresh Kumar Sundararajan |
| collection | DOAJ |
| description | Introduction: Cancer ranks third among the ten leading global
causes of death. To evaluate the genotoxic risks, observed as
DNA damages, can be assessed by Micronucleus (MN) test.
Aim: To identify the occurrence of MN in normal and cancer
cervix and find the correlation between MN and stage of
cancer.
Materials and Methods: A total of 60 females were included
in the study and visual examination of the cervix was done.
Based on the examination two groups were formed: A- Normal
cervix (n-23) and B- Presence of erosion or growth or ulcer etc.,
in cervix (n-37). Midstream urine sample was collected and
centrifuged from the cases after getting the informed consent.
Slides were prepared from the pellet, were fixed in methanol,
glacial acetic acid fixative and stained with Giemsa and May
Grunwald stain.
Statistical analysis was done by student’s t-test and chi-square
test.
Results: A linear association was noted between the mean MN
count and cancer cervix stage. Almost 18.2% of the Group A
cases had significant MN count. Sensitivity and specificity of
MN count in Group A was 83.8% and 82.6% respectively. The
efficiency was 83.3%.
Conclusion: A statistically significant MN count was seen in
the different stages of cancer cervix. There are cases who had
normal findings on visual inspection of cervix but with significant
MN count are prone for malignant transformation. MN assay is
an easy, non-invasive, cost-effective method and can be used
as a screening test for a large population. |
| first_indexed | 2024-12-11T17:31:59Z |
| format | Article |
| id | doaj.art-145194df5bf94d2bb601cf2586cc1227 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-11T17:31:59Z |
| publishDate | 2017-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-145194df5bf94d2bb601cf2586cc12272022-12-22T00:56:47ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-03-01113XC01XC0310.7860/JCDR/2017/23425.9488Micronucleus Assay in Urothelial Cells in Cancer CervixSuresh Kumar Sundararajan0Pratheepa Sivasankari Natarajan1Kanchana2Assistant Professor, Department of Medical Oncology, Madras Medical College and Hospital, Chennai, Tamil Nadu, India.Associate Professor, Department of Anatomy, SRM Medical College Hospital and Research Centre, Chennai, Tamil Nadu, India.Professor, Department of Pathology, Madras Medical College and Hospital, Chennai, Tamil Nadu, India.Introduction: Cancer ranks third among the ten leading global causes of death. To evaluate the genotoxic risks, observed as DNA damages, can be assessed by Micronucleus (MN) test. Aim: To identify the occurrence of MN in normal and cancer cervix and find the correlation between MN and stage of cancer. Materials and Methods: A total of 60 females were included in the study and visual examination of the cervix was done. Based on the examination two groups were formed: A- Normal cervix (n-23) and B- Presence of erosion or growth or ulcer etc., in cervix (n-37). Midstream urine sample was collected and centrifuged from the cases after getting the informed consent. Slides were prepared from the pellet, were fixed in methanol, glacial acetic acid fixative and stained with Giemsa and May Grunwald stain. Statistical analysis was done by student’s t-test and chi-square test. Results: A linear association was noted between the mean MN count and cancer cervix stage. Almost 18.2% of the Group A cases had significant MN count. Sensitivity and specificity of MN count in Group A was 83.8% and 82.6% respectively. The efficiency was 83.3%. Conclusion: A statistically significant MN count was seen in the different stages of cancer cervix. There are cases who had normal findings on visual inspection of cervix but with significant MN count are prone for malignant transformation. MN assay is an easy, non-invasive, cost-effective method and can be used as a screening test for a large population.https://jcdr.net/articles/PDF/9488/23425_CE[Ra1]_F(RK)_PF1(SYGH)_PFA(GH)_PF2(NE_SY_DK).pdfcervical malignancygenotoxicitynuclear abnormalities |
| spellingShingle | Suresh Kumar Sundararajan Pratheepa Sivasankari Natarajan Kanchana Micronucleus Assay in Urothelial Cells in Cancer Cervix Journal of Clinical and Diagnostic Research cervical malignancy genotoxicity nuclear abnormalities |
| title | Micronucleus Assay in Urothelial Cells in Cancer Cervix |
| title_full | Micronucleus Assay in Urothelial Cells in Cancer Cervix |
| title_fullStr | Micronucleus Assay in Urothelial Cells in Cancer Cervix |
| title_full_unstemmed | Micronucleus Assay in Urothelial Cells in Cancer Cervix |
| title_short | Micronucleus Assay in Urothelial Cells in Cancer Cervix |
| title_sort | micronucleus assay in urothelial cells in cancer cervix |
| topic | cervical malignancy genotoxicity nuclear abnormalities |
| url | https://jcdr.net/articles/PDF/9488/23425_CE[Ra1]_F(RK)_PF1(SYGH)_PFA(GH)_PF2(NE_SY_DK).pdf |
| work_keys_str_mv | AT sureshkumarsundararajan micronucleusassayinurothelialcellsincancercervix AT pratheepasivasankarinatarajan micronucleusassayinurothelialcellsincancercervix AT kanchana micronucleusassayinurothelialcellsincancercervix |