Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases
Alkaline phosphatase (ALP) is one of the vital phospho-ester bond cleaving biocatalysts that has inevitable significance in cellular systems, viz., early-stage osteoblast differentiation, cell integrity in tissues, bone mineralization, cancer biomarker, liver dysfunction, cellular osmotic pressure,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Symmetry |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-8994/14/8/1634 |
| _version_ | 1797441665005256704 |
|---|---|
| author | Madhusudan Dasnur Nanjappa Anup Pandith Svetlana Sankaran Dorothy Priyanka Dorairaj Anusha Anjaneya Reddy Hari Prasad Badubanahalli Ramesh |
| author_facet | Madhusudan Dasnur Nanjappa Anup Pandith Svetlana Sankaran Dorothy Priyanka Dorairaj Anusha Anjaneya Reddy Hari Prasad Badubanahalli Ramesh |
| author_sort | Madhusudan Dasnur Nanjappa |
| collection | DOAJ |
| description | Alkaline phosphatase (ALP) is one of the vital phospho-ester bond cleaving biocatalysts that has inevitable significance in cellular systems, viz., early-stage osteoblast differentiation, cell integrity in tissues, bone mineralization, cancer biomarker, liver dysfunction, cellular osmotic pressure, protein folding and many more. Variation from optimal levels of ALP in intra and extracellular fluids can cause severe diseases, including death. Due to these reasons, ALP is considered as a vital biomarker for various preclinical and medical diagnosis. Fluorescence image-based diagnosis is the most widely used method, owing to its simplicity, robustness, non-invasive properties and excellent spatio-temporal resolution (up to the nM/pM level), as compared to conventional analytical techniques, such as the electroanalytical method, nuclear magnetic resonance (NMR) and high-performance liquid chromatography (HPLC). Most of the reviews reported for ALP’s recognition in the literature scarcely explain the structurally related, photophysical and biophysical parameters; and the sub-cellular localizations. Considering these facts, in order to enhance the opto-analytical parameters of fluorescence-based diagnostic materials at the cellular level, herein we have systematically documented recent developments in the opto-analytical capabilities of quencher-free probes for ALP, used in in vitro (biological buffers) to in cellulo conditions, along with in vivo models. |
| first_indexed | 2024-03-09T12:26:27Z |
| format | Article |
| id | doaj.art-14530735e9a646c996070a885133b829 |
| institution | Directory Open Access Journal |
| issn | 2073-8994 |
| language | English |
| last_indexed | 2024-03-09T12:26:27Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Symmetry |
| spelling | doaj.art-14530735e9a646c996070a885133b8292023-11-30T22:33:41ZengMDPI AGSymmetry2073-89942022-08-01148163410.3390/sym14081634Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline PhosphatasesMadhusudan Dasnur Nanjappa0Anup Pandith1Svetlana Sankaran2Dorothy Priyanka Dorairaj3Anusha Anjaneya Reddy4Hari Prasad Badubanahalli Ramesh5Department of Chemistry, University of Mysore, Manasagangotri, Mysuru 570005, IndiaCollege of Biomedical Engineering, Taipei Medical University, Taipei City 11031, TaiwanDepartment of Chemistry, University of Mysore, Manasagangotri, Mysuru 570005, IndiaDepartment of Chemistry, National Institute of Technology, Tiruchirapalli 620015, IndiaDepartment of Chemical Engineering, National Institute of Technology, Surathkal 575025, IndiaDepartment of Chemistry, JC Technical Campus, JSSS & T University, Mysuru 570006, IndiaAlkaline phosphatase (ALP) is one of the vital phospho-ester bond cleaving biocatalysts that has inevitable significance in cellular systems, viz., early-stage osteoblast differentiation, cell integrity in tissues, bone mineralization, cancer biomarker, liver dysfunction, cellular osmotic pressure, protein folding and many more. Variation from optimal levels of ALP in intra and extracellular fluids can cause severe diseases, including death. Due to these reasons, ALP is considered as a vital biomarker for various preclinical and medical diagnosis. Fluorescence image-based diagnosis is the most widely used method, owing to its simplicity, robustness, non-invasive properties and excellent spatio-temporal resolution (up to the nM/pM level), as compared to conventional analytical techniques, such as the electroanalytical method, nuclear magnetic resonance (NMR) and high-performance liquid chromatography (HPLC). Most of the reviews reported for ALP’s recognition in the literature scarcely explain the structurally related, photophysical and biophysical parameters; and the sub-cellular localizations. Considering these facts, in order to enhance the opto-analytical parameters of fluorescence-based diagnostic materials at the cellular level, herein we have systematically documented recent developments in the opto-analytical capabilities of quencher-free probes for ALP, used in in vitro (biological buffers) to in cellulo conditions, along with in vivo models.https://www.mdpi.com/2073-8994/14/8/1634osteoblastbone mineralizationhomeostasisspatio-temporal resolutionsfluorescent materialsin cellulo |
| spellingShingle | Madhusudan Dasnur Nanjappa Anup Pandith Svetlana Sankaran Dorothy Priyanka Dorairaj Anusha Anjaneya Reddy Hari Prasad Badubanahalli Ramesh Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases Symmetry osteoblast bone mineralization homeostasis spatio-temporal resolutions fluorescent materials in cellulo |
| title | Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases |
| title_full | Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases |
| title_fullStr | Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases |
| title_full_unstemmed | Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases |
| title_short | Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases |
| title_sort | recent advancements in developments of novel fluorescent probes in cellulo recognitions of alkaline phosphatases |
| topic | osteoblast bone mineralization homeostasis spatio-temporal resolutions fluorescent materials in cellulo |
| url | https://www.mdpi.com/2073-8994/14/8/1634 |
| work_keys_str_mv | AT madhusudandasnurnanjappa recentadvancementsindevelopmentsofnovelfluorescentprobesincellulorecognitionsofalkalinephosphatases AT anuppandith recentadvancementsindevelopmentsofnovelfluorescentprobesincellulorecognitionsofalkalinephosphatases AT svetlanasankaran recentadvancementsindevelopmentsofnovelfluorescentprobesincellulorecognitionsofalkalinephosphatases AT dorothypriyankadorairaj recentadvancementsindevelopmentsofnovelfluorescentprobesincellulorecognitionsofalkalinephosphatases AT anushaanjaneyareddy recentadvancementsindevelopmentsofnovelfluorescentprobesincellulorecognitionsofalkalinephosphatases AT hariprasadbadubanahalliramesh recentadvancementsindevelopmentsofnovelfluorescentprobesincellulorecognitionsofalkalinephosphatases |