Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition
Drought stress is the primary constraint on plant-based food production, particularly paddy production. Several studies have examined plant resistance to heat and osmotic pressure. This study aimed to isolate bacteria with plant growth-promoting properties that could tolerate high temperatures and i...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2024-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/effectiveness-of-the-drought-tolerant-plant-growth-promoting-rhizobacteria-pgpr-that-supports-paddy-growth-in-drought-condition/ |
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| author | Emmyrafedziawati Aida Kamal Rafedzi Izwan Bharudin Shazilah Kamaruddin Farah Diba Abu Bakar Abdul Munir Abdul Murad |
| author_facet | Emmyrafedziawati Aida Kamal Rafedzi Izwan Bharudin Shazilah Kamaruddin Farah Diba Abu Bakar Abdul Munir Abdul Murad |
| author_sort | Emmyrafedziawati Aida Kamal Rafedzi |
| collection | DOAJ |
| description | Drought stress is the primary constraint on plant-based food production, particularly paddy production. Several studies have examined plant resistance to heat and osmotic pressure. This study aimed to isolate bacteria with plant growth-promoting properties that could tolerate high temperatures and improve paddy growth during drought. Five isolates with a high optical density value (OD600) at 30% PEG 6000 (equivalent to -1.03MPa) and able to grow at high temperatures were identified based on the 16S rRNA gene sequences as Achromobacter spanius UKM UR10, Bacillus pumillus UKM UR11, Bacillus cereus UKM R66, and Bacillus altitudinis UKM RB11, which were isolated from the root, where as Bacillus sp. UKM S8 was isolated from rhizosphere soil. These isolates exhibited 1-amino cyclopropane-1-carboxylate (ACC) deaminase activities ranging between 1.01 and 1.12 mmol α-ketobutyrate mg-1 protein h-1, which degraded ACC to α-ketobutyrate and ammonia. Other plant growth promoters assessed include indole acetic acid (IAA; concentration between 9.69 and 13.15µg/ml) and phosphate solubilization (concentrations between 31.74 and 51.30 mg/l) production. Subsequently, the selected plant growth-promoting rhizobacteria (PGPR) were incorporated as a consortium and inoculated on paddy seeds, thus increasing total chlorophyll, proline, and soluble sugar content in paddy subjected to drought-stress conditions. Paddy yield components and performances, such as panicle number, spikelet number, dry grain weight, number of leaves, stalk length, and root length increased significantly. This isolated PGPR exhibits heat resistance, promotes plant growth, and can serve as an inoculant for paddy plants under drought conditions. |
| first_indexed | 2024-04-24T22:45:26Z |
| format | Article |
| id | doaj.art-1454e79aedc944b5a502e7621ae1775e |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-24T22:45:26Z |
| publishDate | 2024-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-1454e79aedc944b5a502e7621ae1775e2024-03-18T11:13:43ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-03-01181297310https://doi.org/10.22207/JPAM.18.1.13Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought ConditionEmmyrafedziawati Aida Kamal Rafedzihttps://orcid.org/0000-0002-2888-0324Izwan Bharudinhttps://orcid.org/0000-0002-8908-4786Shazilah Kamaruddinhttps://orcid.org/0000-0003-3017-7045Farah Diba Abu Bakarhttps://orcid.org/0000-0002-6416-7470Abdul Munir Abdul Muradhttps://orcid.org/0000-0001-6402-7198Drought stress is the primary constraint on plant-based food production, particularly paddy production. Several studies have examined plant resistance to heat and osmotic pressure. This study aimed to isolate bacteria with plant growth-promoting properties that could tolerate high temperatures and improve paddy growth during drought. Five isolates with a high optical density value (OD600) at 30% PEG 6000 (equivalent to -1.03MPa) and able to grow at high temperatures were identified based on the 16S rRNA gene sequences as Achromobacter spanius UKM UR10, Bacillus pumillus UKM UR11, Bacillus cereus UKM R66, and Bacillus altitudinis UKM RB11, which were isolated from the root, where as Bacillus sp. UKM S8 was isolated from rhizosphere soil. These isolates exhibited 1-amino cyclopropane-1-carboxylate (ACC) deaminase activities ranging between 1.01 and 1.12 mmol α-ketobutyrate mg-1 protein h-1, which degraded ACC to α-ketobutyrate and ammonia. Other plant growth promoters assessed include indole acetic acid (IAA; concentration between 9.69 and 13.15µg/ml) and phosphate solubilization (concentrations between 31.74 and 51.30 mg/l) production. Subsequently, the selected plant growth-promoting rhizobacteria (PGPR) were incorporated as a consortium and inoculated on paddy seeds, thus increasing total chlorophyll, proline, and soluble sugar content in paddy subjected to drought-stress conditions. Paddy yield components and performances, such as panicle number, spikelet number, dry grain weight, number of leaves, stalk length, and root length increased significantly. This isolated PGPR exhibits heat resistance, promotes plant growth, and can serve as an inoculant for paddy plants under drought conditions.https://microbiologyjournal.org/effectiveness-of-the-drought-tolerant-plant-growth-promoting-rhizobacteria-pgpr-that-supports-paddy-growth-in-drought-condition/plant growth promoterdrought stressacc deaminase enzymephosphate-solubilizing bacteria |
| spellingShingle | Emmyrafedziawati Aida Kamal Rafedzi Izwan Bharudin Shazilah Kamaruddin Farah Diba Abu Bakar Abdul Munir Abdul Murad Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition Journal of Pure and Applied Microbiology plant growth promoter drought stress acc deaminase enzyme phosphate-solubilizing bacteria |
| title | Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition |
| title_full | Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition |
| title_fullStr | Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition |
| title_full_unstemmed | Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition |
| title_short | Effectiveness of the Drought-tolerant Plant Growth Promoting Rhizobacteria (PGPR) that Supports Paddy Growth in Drought Condition |
| title_sort | effectiveness of the drought tolerant plant growth promoting rhizobacteria pgpr that supports paddy growth in drought condition |
| topic | plant growth promoter drought stress acc deaminase enzyme phosphate-solubilizing bacteria |
| url | https://microbiologyjournal.org/effectiveness-of-the-drought-tolerant-plant-growth-promoting-rhizobacteria-pgpr-that-supports-paddy-growth-in-drought-condition/ |
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