Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology
In the field of bone tissue engineering, porous hydroxyapatite (HA) bone repair scaffolds with controlled architecture and porosity are challenging to manufacture and lack sufficient osteoinductive activity, which limit its wider clinical application. This study fabricated porous graphene oxide (GO)...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S026412752200853X |
| _version_ | 1811249596553232384 |
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| author | Hongyu Zhao Hongyu Xing Qingguo Lai Yixuan Zhao Qinghua Chen Bin Zou |
| author_facet | Hongyu Zhao Hongyu Xing Qingguo Lai Yixuan Zhao Qinghua Chen Bin Zou |
| author_sort | Hongyu Zhao |
| collection | DOAJ |
| description | In the field of bone tissue engineering, porous hydroxyapatite (HA) bone repair scaffolds with controlled architecture and porosity are challenging to manufacture and lack sufficient osteoinductive activity, which limit its wider clinical application. This study fabricated porous graphene oxide (GO)/HA composite ceramic scaffolds using digital light processing (DLP) technology. The properties of the composite ceramic slurry and the process parameters of curing, degreasing, and sintering were initially examined to ensure molding accuracy. The research showed that a small amount of GO (0.1–0.4 wt%) could better maintain the pore structure and improve the mechanical properties of composite ceramics. Porous scaffolds with pore sizes of 300–400 μm were prepared for co-culture with rat bone marrow mesenchymal stem cells to detect biocompatibility. The results showed that all scaffolds have no cytotoxicity. Compared with HA, the GO/HA ceramic scaffold significantly promoted the cell adhesion, proliferation, and expression of osteogenesis-related genes; GO (0.1–0.2 wt%) /HA scaffolds exhibited superior alkaline phosphatase activity and more effective bone mineralization, showing enhanced osteoinductivity. The porous GO/HA composite scaffold fabricated by DLP presented a strong potential for repairing bone defects. |
| first_indexed | 2024-04-12T15:49:20Z |
| format | Article |
| id | doaj.art-1457de73ca7541db8530dad34427f6d4 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-12T15:49:20Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-1457de73ca7541db8530dad34427f6d42022-12-22T03:26:33ZengElsevierMaterials & Design0264-12752022-11-01223111231Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technologyHongyu Zhao0Hongyu Xing1Qingguo Lai2Yixuan Zhao3Qinghua Chen4Bin Zou5Department of Oral and Maxillofacial Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China; School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, 250012, ChinaSchool of Mechanical and Electronic Engineering, Shandong Jianzhu University, Jinan, People's Republic of China; Center for Advanced Jet Engineering Technology (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, People's Republic of China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Shandong University, Ministry of Education, People's Republic of China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), People's Republic of ChinaDepartment of Oral and Maxillofacial Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China; Research Center of 3D Printing in Stomatology of Shandong University, People's Republic of China; Corresponding author at: The Second Hospital of Shandong University, 247, Beiyuan Street, Jinan 25033, China.Department of Oral and Maxillofacial Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China; School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, 250012, ChinaCenter for Advanced Jet Engineering Technology (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, People's Republic of China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Shandong University, Ministry of Education, People's Republic of China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), People's Republic of ChinaCenter for Advanced Jet Engineering Technology (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, People's Republic of China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Shandong University, Ministry of Education, People's Republic of China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), People's Republic of ChinaIn the field of bone tissue engineering, porous hydroxyapatite (HA) bone repair scaffolds with controlled architecture and porosity are challenging to manufacture and lack sufficient osteoinductive activity, which limit its wider clinical application. This study fabricated porous graphene oxide (GO)/HA composite ceramic scaffolds using digital light processing (DLP) technology. The properties of the composite ceramic slurry and the process parameters of curing, degreasing, and sintering were initially examined to ensure molding accuracy. The research showed that a small amount of GO (0.1–0.4 wt%) could better maintain the pore structure and improve the mechanical properties of composite ceramics. Porous scaffolds with pore sizes of 300–400 μm were prepared for co-culture with rat bone marrow mesenchymal stem cells to detect biocompatibility. The results showed that all scaffolds have no cytotoxicity. Compared with HA, the GO/HA ceramic scaffold significantly promoted the cell adhesion, proliferation, and expression of osteogenesis-related genes; GO (0.1–0.2 wt%) /HA scaffolds exhibited superior alkaline phosphatase activity and more effective bone mineralization, showing enhanced osteoinductivity. The porous GO/HA composite scaffold fabricated by DLP presented a strong potential for repairing bone defects.http://www.sciencedirect.com/science/article/pii/S026412752200853XHydroxyapatite (HA)Graphene oxide (GO)Digital light processingCurabilityMolding qualityOsteogenic activity |
| spellingShingle | Hongyu Zhao Hongyu Xing Qingguo Lai Yixuan Zhao Qinghua Chen Bin Zou Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology Materials & Design Hydroxyapatite (HA) Graphene oxide (GO) Digital light processing Curability Molding quality Osteogenic activity |
| title | Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology |
| title_full | Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology |
| title_fullStr | Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology |
| title_full_unstemmed | Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology |
| title_short | Additive manufacturing of graphene oxide/hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology |
| title_sort | additive manufacturing of graphene oxide hydroxyapatite bioceramic scaffolds with reinforced osteoinductivity based on digital light processing technology |
| topic | Hydroxyapatite (HA) Graphene oxide (GO) Digital light processing Curability Molding quality Osteogenic activity |
| url | http://www.sciencedirect.com/science/article/pii/S026412752200853X |
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