Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes).
Small teleosts have recently been established as models of human diseases. However, measuring heart rate by electrocardiography is highly invasive for small fish and not widely used. The physiological nature and function of vertebrate autonomic nervous system (ANS) modulation of the heart has tradit...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0273064 |
| _version_ | 1797956083822624768 |
|---|---|
| author | Tomomi Watanabe-Asaka Maki Niihori Hiroki Sonobe Kento Igarashi Shoji Oda Ken-Ichi Iwasaki Yoshihiko Katada Toshikazu Yamashita Masahiro Terada Shoji A Baba Hiroshi Mitani Chiaki Mukai |
| author_facet | Tomomi Watanabe-Asaka Maki Niihori Hiroki Sonobe Kento Igarashi Shoji Oda Ken-Ichi Iwasaki Yoshihiko Katada Toshikazu Yamashita Masahiro Terada Shoji A Baba Hiroshi Mitani Chiaki Mukai |
| author_sort | Tomomi Watanabe-Asaka |
| collection | DOAJ |
| description | Small teleosts have recently been established as models of human diseases. However, measuring heart rate by electrocardiography is highly invasive for small fish and not widely used. The physiological nature and function of vertebrate autonomic nervous system (ANS) modulation of the heart has traditionally been investigated in larvae, transparent but with an immature ANS, or in anesthetized adults, whose ANS activity may possibly be disturbed under anesthesia. Here, we defined the frequency characteristics of heart rate variability (HRV) modulated by the ANS from observations of heart movement in high-speed movie images and changes in ANS regulation under environmental stimulation in unanesthetized adult medaka (Oryzias latipes). The HRV was significantly reduced by atropine (1 mM) in the 0.25-0.65 Hz and by propranolol (100 μM) at 0.65-1.25 Hz range, suggesting that HRV in adult medaka is modulated by both the parasympathetic and sympathetic nervous systems within these frequency ranges. Such modulations of HRV by the ANS in adult medaka were remarkably suppressed under anesthesia and continuous exposure to light suppressed HRV only in the 0.25-0.65 Hz range, indicating parasympathetic withdrawal. Furthermore, pre-hatching embryos did not show HRV and the power of HRV developed as fish grew. These results strongly suggest that ANS modulation of the heart in adult medaka is frequency-dependent phenomenon, and that the impact of long-term environmental stimuli on ANS activities, in addition to development of ANS activities, can be precisely evaluated in medaka using the presented method. |
| first_indexed | 2024-04-10T23:44:22Z |
| format | Article |
| id | doaj.art-145a2526a718456f99bad93412d08805 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T23:44:22Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-145a2526a718456f99bad93412d088052023-01-11T05:31:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712e027306410.1371/journal.pone.0273064Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes).Tomomi Watanabe-AsakaMaki NiihoriHiroki SonobeKento IgarashiShoji OdaKen-Ichi IwasakiYoshihiko KatadaToshikazu YamashitaMasahiro TeradaShoji A BabaHiroshi MitaniChiaki MukaiSmall teleosts have recently been established as models of human diseases. However, measuring heart rate by electrocardiography is highly invasive for small fish and not widely used. The physiological nature and function of vertebrate autonomic nervous system (ANS) modulation of the heart has traditionally been investigated in larvae, transparent but with an immature ANS, or in anesthetized adults, whose ANS activity may possibly be disturbed under anesthesia. Here, we defined the frequency characteristics of heart rate variability (HRV) modulated by the ANS from observations of heart movement in high-speed movie images and changes in ANS regulation under environmental stimulation in unanesthetized adult medaka (Oryzias latipes). The HRV was significantly reduced by atropine (1 mM) in the 0.25-0.65 Hz and by propranolol (100 μM) at 0.65-1.25 Hz range, suggesting that HRV in adult medaka is modulated by both the parasympathetic and sympathetic nervous systems within these frequency ranges. Such modulations of HRV by the ANS in adult medaka were remarkably suppressed under anesthesia and continuous exposure to light suppressed HRV only in the 0.25-0.65 Hz range, indicating parasympathetic withdrawal. Furthermore, pre-hatching embryos did not show HRV and the power of HRV developed as fish grew. These results strongly suggest that ANS modulation of the heart in adult medaka is frequency-dependent phenomenon, and that the impact of long-term environmental stimuli on ANS activities, in addition to development of ANS activities, can be precisely evaluated in medaka using the presented method.https://doi.org/10.1371/journal.pone.0273064 |
| spellingShingle | Tomomi Watanabe-Asaka Maki Niihori Hiroki Sonobe Kento Igarashi Shoji Oda Ken-Ichi Iwasaki Yoshihiko Katada Toshikazu Yamashita Masahiro Terada Shoji A Baba Hiroshi Mitani Chiaki Mukai Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes). PLoS ONE |
| title | Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes). |
| title_full | Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes). |
| title_fullStr | Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes). |
| title_full_unstemmed | Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes). |
| title_short | Acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka (Oryzias latipes). |
| title_sort | acquirement of the autonomic nervous system modulation evaluated by heart rate variability in medaka oryzias latipes |
| url | https://doi.org/10.1371/journal.pone.0273064 |
| work_keys_str_mv | AT tomomiwatanabeasaka acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT makiniihori acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT hirokisonobe acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT kentoigarashi acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT shojioda acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT kenichiiwasaki acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT yoshihikokatada acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT toshikazuyamashita acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT masahiroterada acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT shojiababa acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT hiroshimitani acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes AT chiakimukai acquirementoftheautonomicnervoussystemmodulationevaluatedbyheartratevariabilityinmedakaoryziaslatipes |