Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors
Organ transplantation is a life-saving procedure and the preferred method of treatment for a growing number of disease states. The advent of new immunosuppressants and improved care has led to great advances in both patient and graft survival. However, acute T-cell-mediated graft rejection occurs in...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2013-10-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X657963 |
| _version_ | 1818969852265627648 |
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| author | Kimberly S. Butler Debbie M. Lovato Natalie L. Adolphi Robert Belfon Danielle L. Fegan Todd C. Monson Helen J. Hathaway Dale L. Huber T. E. Tessier H. C. Bryant Edward R. Flynn Richard S. Larson M.D., Ph.D. |
| author_facet | Kimberly S. Butler Debbie M. Lovato Natalie L. Adolphi Robert Belfon Danielle L. Fegan Todd C. Monson Helen J. Hathaway Dale L. Huber T. E. Tessier H. C. Bryant Edward R. Flynn Richard S. Larson M.D., Ph.D. |
| author_sort | Kimberly S. Butler |
| collection | DOAJ |
| description | Organ transplantation is a life-saving procedure and the preferred method of treatment for a growing number of disease states. The advent of new immunosuppressants and improved care has led to great advances in both patient and graft survival. However, acute T-cell-mediated graft rejection occurs in a significant quantity of recipients and remains a life-threatening condition. Acute rejection is associated with decrease in long-term graft survival, demonstrating a need to carefully monitor transplant patients. Current diagnostic criteria for transplant rejection rely on invasive tissue biopsies or relatively nonspecific clinical features. A noninvasive way is needed to detect, localize, and monitor transplant rejection. Capitalizing on advances in targeted contrast agents and magnetic-based detection technology, we developed anti-CD3 antibody-tagged nanoparticles. T cells were found to bind preferentially to antibody-tagged nanoparticles, as identified through light microscopy, transmission electron microscopy, and confocal microscopy. Using mouse skin graft models, we were also able to demonstrate in vivo vascular delivery of T-cell targeted nanoparticles. We conclude that targeting lymphocytes with magnetic nanoparticles is conducive to developing a novel, noninvasive strategy for identifying transplant rejection. |
| first_indexed | 2024-12-20T14:27:10Z |
| format | Article |
| id | doaj.art-145b2a46015d4263b09d28195e2adb03 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-20T14:27:10Z |
| publishDate | 2013-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-145b2a46015d4263b09d28195e2adb032022-12-21T19:37:44ZengSAGE PublishingCell Transplantation0963-68971555-38922013-10-012210.3727/096368912X657963Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic SensorsKimberly S. Butler0Debbie M. Lovato1Natalie L. Adolphi2Robert Belfon3Danielle L. Fegan4Todd C. Monson5Helen J. Hathaway6Dale L. Huber7T. E. Tessier8H. C. Bryant9Edward R. Flynn10Richard S. Larson M.D., Ph.D.11Department of Pathology, University of New Mexico, and Cancer Research and Treatment Center, Albuquerque, NM, USADepartment of Pathology, University of New Mexico, and Cancer Research and Treatment Center, Albuquerque, NM, USADepartment of Biochemistry and Molecular Biology, University of New Mexico, Albuquerque, NM, USADepartment of Cell Biology and Physiology, University of New Mexico, Albuquerque, NM, USASenior Scientific, LLC, Albuquerque, NM, USANanomaterials Sciences Department, Sandia National Laboratories, Albuquerque, NM, USADepartment of Cell Biology and Physiology, University of New Mexico, Albuquerque, NM, USACenter for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, NM, USASenior Scientific, LLC, Albuquerque, NM, USASenior Scientific, LLC, Albuquerque, NM, USASenior Scientific, LLC, Albuquerque, NM, USADepartment of Pathology, University of New Mexico, and Cancer Research and Treatment Center, Albuquerque, NM, USAOrgan transplantation is a life-saving procedure and the preferred method of treatment for a growing number of disease states. The advent of new immunosuppressants and improved care has led to great advances in both patient and graft survival. However, acute T-cell-mediated graft rejection occurs in a significant quantity of recipients and remains a life-threatening condition. Acute rejection is associated with decrease in long-term graft survival, demonstrating a need to carefully monitor transplant patients. Current diagnostic criteria for transplant rejection rely on invasive tissue biopsies or relatively nonspecific clinical features. A noninvasive way is needed to detect, localize, and monitor transplant rejection. Capitalizing on advances in targeted contrast agents and magnetic-based detection technology, we developed anti-CD3 antibody-tagged nanoparticles. T cells were found to bind preferentially to antibody-tagged nanoparticles, as identified through light microscopy, transmission electron microscopy, and confocal microscopy. Using mouse skin graft models, we were also able to demonstrate in vivo vascular delivery of T-cell targeted nanoparticles. We conclude that targeting lymphocytes with magnetic nanoparticles is conducive to developing a novel, noninvasive strategy for identifying transplant rejection.https://doi.org/10.3727/096368912X657963 |
| spellingShingle | Kimberly S. Butler Debbie M. Lovato Natalie L. Adolphi Robert Belfon Danielle L. Fegan Todd C. Monson Helen J. Hathaway Dale L. Huber T. E. Tessier H. C. Bryant Edward R. Flynn Richard S. Larson M.D., Ph.D. Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors Cell Transplantation |
| title | Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors |
| title_full | Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors |
| title_fullStr | Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors |
| title_full_unstemmed | Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors |
| title_short | Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors |
| title_sort | development of antibody tagged nanoparticles for detection of transplant rejection using biomagnetic sensors |
| url | https://doi.org/10.3727/096368912X657963 |
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