Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family th...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0255470 |
| _version_ | 1818398948707008512 |
|---|---|
| author | Phuong Nguyen Duy Dai Tran Lan Hang Pham Thu Huong Phung Thi Thu Ha Nguyen Thanh Ngoc Phuong Pham Florence Auguy Huong Bui Thi Thu Tran Bao Manh Sebastien Cunnac Xuan Hoi Pham |
| author_facet | Phuong Nguyen Duy Dai Tran Lan Hang Pham Thu Huong Phung Thi Thu Ha Nguyen Thanh Ngoc Phuong Pham Florence Auguy Huong Bui Thi Thu Tran Bao Manh Sebastien Cunnac Xuan Hoi Pham |
| author_sort | Phuong Nguyen Duy |
| collection | DOAJ |
| description | TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family that encodes sugar transporters. Together with other Clade III members, it behaves as a susceptibility (S) gene whose induction by Asian Xoo Transcription-Activator-Like Effectors (TALEs) is absolutely necessary for disease. In this study, we sought to introduce BLB resistance in the TBR225 elite variety. First, two Vietnamese Xoo strains were shown to up-regulate OsSWEET14 upon TBR225 infection. To investigate if this induction is connected with disease susceptibility, nine TBR225 mutant lines with mutations in the AvrXa7, PthXo3 or TalF TALEs DNA target sequences of the OsSWEET14 promoter were obtained using the CRISPR/Cas9 editing system. Genotyping analysis of T0 and T1 individuals showed that mutations were stably inherited. None of the examined agronomic traits of three transgene-free T2 edited lines were significantly different from those of wild-type TBR225. Importantly, one of these T2 lines, harboring the largest homozygous 6-bp deletion, displayed decreased OsSWEET14 expression as well as a significantly reduced susceptibility to a Vietnamese Xoo strains and complete resistance to another one. Our findings indicate that CRISPR/Cas9 editing conferred an improved BLB resistance to a Vietnamese commercial elite rice variety. |
| first_indexed | 2024-12-14T07:12:54Z |
| format | Article |
| id | doaj.art-145dce7282184ef4967d27e9f8990353 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:12:54Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-145dce7282184ef4967d27e9f89903532022-12-21T23:11:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01169e025547010.1371/journal.pone.0255470Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.Phuong Nguyen DuyDai Tran LanHang Pham ThuHuong Phung Thi ThuHa Nguyen ThanhNgoc Phuong PhamFlorence AuguyHuong Bui Thi ThuTran Bao ManhSebastien CunnacXuan Hoi PhamTBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family that encodes sugar transporters. Together with other Clade III members, it behaves as a susceptibility (S) gene whose induction by Asian Xoo Transcription-Activator-Like Effectors (TALEs) is absolutely necessary for disease. In this study, we sought to introduce BLB resistance in the TBR225 elite variety. First, two Vietnamese Xoo strains were shown to up-regulate OsSWEET14 upon TBR225 infection. To investigate if this induction is connected with disease susceptibility, nine TBR225 mutant lines with mutations in the AvrXa7, PthXo3 or TalF TALEs DNA target sequences of the OsSWEET14 promoter were obtained using the CRISPR/Cas9 editing system. Genotyping analysis of T0 and T1 individuals showed that mutations were stably inherited. None of the examined agronomic traits of three transgene-free T2 edited lines were significantly different from those of wild-type TBR225. Importantly, one of these T2 lines, harboring the largest homozygous 6-bp deletion, displayed decreased OsSWEET14 expression as well as a significantly reduced susceptibility to a Vietnamese Xoo strains and complete resistance to another one. Our findings indicate that CRISPR/Cas9 editing conferred an improved BLB resistance to a Vietnamese commercial elite rice variety.https://doi.org/10.1371/journal.pone.0255470 |
| spellingShingle | Phuong Nguyen Duy Dai Tran Lan Hang Pham Thu Huong Phung Thi Thu Ha Nguyen Thanh Ngoc Phuong Pham Florence Auguy Huong Bui Thi Thu Tran Bao Manh Sebastien Cunnac Xuan Hoi Pham Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. PLoS ONE |
| title | Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. |
| title_full | Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. |
| title_fullStr | Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. |
| title_full_unstemmed | Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. |
| title_short | Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. |
| title_sort | improved bacterial leaf blight disease resistance in the major elite vietnamese rice cultivar tbr225 via editing of the ossweet14 promoter |
| url | https://doi.org/10.1371/journal.pone.0255470 |
| work_keys_str_mv | AT phuongnguyenduy improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT daitranlan improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT hangphamthu improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT huongphungthithu improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT hanguyenthanh improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT ngocphuongpham improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT florenceauguy improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT huongbuithithu improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT tranbaomanh improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT sebastiencunnac improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT xuanhoipham improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter |