Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands
Abstract Tick-borne encephalitis virus (TBEV) may cause tick-borne encephalitis (TBE), a potential life-threatening infection of the central nervous system in humans. Phylogenetically, TBEVs can be subdivided into three main subtypes, which differ in endemic region and pathogenic potential. In 2016,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29075-0 |
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| author | Tabitha E. Hoornweg Gert-Jan Godeke Marieke N. Hoogerwerf Puck B. van Kasteren Ankje de Vries Hein Sprong Georges M. G. M. Verjans Debby van Riel Johan H. J. Reimerink Barry Rockx Chantal B. E. M. Reusken |
| author_facet | Tabitha E. Hoornweg Gert-Jan Godeke Marieke N. Hoogerwerf Puck B. van Kasteren Ankje de Vries Hein Sprong Georges M. G. M. Verjans Debby van Riel Johan H. J. Reimerink Barry Rockx Chantal B. E. M. Reusken |
| author_sort | Tabitha E. Hoornweg |
| collection | DOAJ |
| description | Abstract Tick-borne encephalitis virus (TBEV) may cause tick-borne encephalitis (TBE), a potential life-threatening infection of the central nervous system in humans. Phylogenetically, TBEVs can be subdivided into three main subtypes, which differ in endemic region and pathogenic potential. In 2016, TBEV was first detected in the Netherlands. One of two detected strains, referred to as Salland, belonged to the TBEV-Eu subtype, yet diverged ≥ 2% on amino acid level from other members of this subtype. Here, we report the successful rescue of this strain using infectious subgenomic amplicons and its subsequent in vitro characterization by comparison to two well-characterized TBEV-Eu strains; Neudoerfl and Hypr. In the human alveolar epithelial cell line A549, growth kinetics of Salland were comparable to the high pathogenicity TBEV-Eu strain Hypr, and both strains grew considerably faster than the mildly pathogenic strain Neudoerfl. In the human neuroblastoma cell line SK-N-SH, Salland replicated faster and to higher infectious titers than both reference strains. All three TBEV strains infected primary human monocyte-derived dendritic cells to a similar extent and interacted with the type I interferon system in a similar manner. The current study serves as the first in vitro characterization of the novel, divergent TBEV-Eu strain Salland. |
| first_indexed | 2024-04-09T22:55:46Z |
| format | Article |
| id | doaj.art-145dec037d5c4db79184c779c3e0381d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:55:46Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-145dec037d5c4db79184c779c3e0381d2023-03-22T11:17:09ZengNature PortfolioScientific Reports2045-23222023-02-0113111410.1038/s41598-023-29075-0Rescue and in vitro characterization of a divergent TBEV-Eu strain from the NetherlandsTabitha E. Hoornweg0Gert-Jan Godeke1Marieke N. Hoogerwerf2Puck B. van Kasteren3Ankje de Vries4Hein Sprong5Georges M. G. M. Verjans6Debby van Riel7Johan H. J. Reimerink8Barry Rockx9Chantal B. E. M. Reusken10Center for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentDepartment of Viroscience, Erasmus University Medical CenterDepartment of Viroscience, Erasmus University Medical CenterCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentCenter for Infectious Disease Control, National Institute for Public Health and the EnvironmentAbstract Tick-borne encephalitis virus (TBEV) may cause tick-borne encephalitis (TBE), a potential life-threatening infection of the central nervous system in humans. Phylogenetically, TBEVs can be subdivided into three main subtypes, which differ in endemic region and pathogenic potential. In 2016, TBEV was first detected in the Netherlands. One of two detected strains, referred to as Salland, belonged to the TBEV-Eu subtype, yet diverged ≥ 2% on amino acid level from other members of this subtype. Here, we report the successful rescue of this strain using infectious subgenomic amplicons and its subsequent in vitro characterization by comparison to two well-characterized TBEV-Eu strains; Neudoerfl and Hypr. In the human alveolar epithelial cell line A549, growth kinetics of Salland were comparable to the high pathogenicity TBEV-Eu strain Hypr, and both strains grew considerably faster than the mildly pathogenic strain Neudoerfl. In the human neuroblastoma cell line SK-N-SH, Salland replicated faster and to higher infectious titers than both reference strains. All three TBEV strains infected primary human monocyte-derived dendritic cells to a similar extent and interacted with the type I interferon system in a similar manner. The current study serves as the first in vitro characterization of the novel, divergent TBEV-Eu strain Salland.https://doi.org/10.1038/s41598-023-29075-0 |
| spellingShingle | Tabitha E. Hoornweg Gert-Jan Godeke Marieke N. Hoogerwerf Puck B. van Kasteren Ankje de Vries Hein Sprong Georges M. G. M. Verjans Debby van Riel Johan H. J. Reimerink Barry Rockx Chantal B. E. M. Reusken Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands Scientific Reports |
| title | Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands |
| title_full | Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands |
| title_fullStr | Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands |
| title_full_unstemmed | Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands |
| title_short | Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands |
| title_sort | rescue and in vitro characterization of a divergent tbev eu strain from the netherlands |
| url | https://doi.org/10.1038/s41598-023-29075-0 |
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