Feline herpesvirus 1 viral load related to environmental factors in sheltered cats
ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454&lng=en&tlng=en |
| _version_ | 1811282612464910336 |
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| author | Natasha Baumworcel Joylson de Jesus Pereira Ana Maria Barros Soares Guilherme Nunes Souza Nadia Regina Pereira Almosny Tatiana Xavier de Castro |
| author_facet | Natasha Baumworcel Joylson de Jesus Pereira Ana Maria Barros Soares Guilherme Nunes Souza Nadia Regina Pereira Almosny Tatiana Xavier de Castro |
| author_sort | Natasha Baumworcel |
| collection | DOAJ |
| description | ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification. |
| first_indexed | 2024-04-13T01:55:43Z |
| format | Article |
| id | doaj.art-145f7be58f5348c4a755e04b45603c13 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-13T01:55:43Z |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-145f7be58f5348c4a755e04b45603c132022-12-22T03:07:46ZengUniversidade Federal de Santa MariaCiência Rural1678-459649510.1590/0103-8478cr20190067S0103-84782019000500454Feline herpesvirus 1 viral load related to environmental factors in sheltered catsNatasha BaumworcelJoylson de Jesus PereiraAna Maria Barros SoaresGuilherme Nunes SouzaNadia Regina Pereira AlmosnyTatiana Xavier de CastroABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454&lng=en&tlng=enFHV-1conjuntivitemedicina de abrigoqPCRabrigo animal |
| spellingShingle | Natasha Baumworcel Joylson de Jesus Pereira Ana Maria Barros Soares Guilherme Nunes Souza Nadia Regina Pereira Almosny Tatiana Xavier de Castro Feline herpesvirus 1 viral load related to environmental factors in sheltered cats Ciência Rural FHV-1 conjuntivite medicina de abrigo qPCR abrigo animal |
| title | Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
| title_full | Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
| title_fullStr | Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
| title_full_unstemmed | Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
| title_short | Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
| title_sort | feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
| topic | FHV-1 conjuntivite medicina de abrigo qPCR abrigo animal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454&lng=en&tlng=en |
| work_keys_str_mv | AT natashabaumworcel felineherpesvirus1viralloadrelatedtoenvironmentalfactorsinshelteredcats AT joylsondejesuspereira felineherpesvirus1viralloadrelatedtoenvironmentalfactorsinshelteredcats AT anamariabarrossoares felineherpesvirus1viralloadrelatedtoenvironmentalfactorsinshelteredcats AT guilhermenunessouza felineherpesvirus1viralloadrelatedtoenvironmentalfactorsinshelteredcats AT nadiareginapereiraalmosny felineherpesvirus1viralloadrelatedtoenvironmentalfactorsinshelteredcats AT tatianaxavierdecastro felineherpesvirus1viralloadrelatedtoenvironmentalfactorsinshelteredcats |