Degenerative effects in rat eyes after experimental ocular hypertension
This study was used to evaluate the degenerative effects on the retina and eye-cup sections after experimental induction of acute ocular hypertension on animal models. In particular, vascular events were directly focused in this research in order to assess the vascular remodeling after transient ocu...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2012-10-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2068 |
| _version_ | 1818270536873017344 |
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| author | G. Scarsella M. Nebbioso S. Stefanini N. Pescosolido |
| author_facet | G. Scarsella M. Nebbioso S. Stefanini N. Pescosolido |
| author_sort | G. Scarsella |
| collection | DOAJ |
| description | This study was used to evaluate the degenerative effects on the retina and eye-cup sections after experimental induction of acute ocular hypertension on animal models. In particular, vascular events were directly focused in this research in order to assess the vascular remodeling after transient ocular hypertension on rat models. After local anaesthesia by administration of eye drops of 0.4% oxibuprocaine, 16 male adult <em>Wistar</em> rats were injected in the anterior chamber of the right eye with 15 µL of methylcellulose (MTC) 2% in physiological solution. The morphology and the vessels of the retina and eye-cup sections were examined in animals sacrificed 72 h after induction of ocular hypertension. In retinal fluorescein angiographies (FAGs), by means of fluorescein isothiocyanate-coniugated dextran (FITC), the radial venules showed enlargements and increased branching, while the arterioles appeared focally thickened. The length and size of actually perfused vessels appeared increased in the whole superficial plexus. In eye-cup sections of MTC-injected animals, in deep plexus and connecting layer there was a bigger increase of vessels than in controls. Moreover, the immunolocalization of astrocytic marker glial fibrillary acidic protein (GFAP) revealed its increased expression in internal limiting membrane and ganglion cell layer, as well as its presence in Müller cells. Finally, the pro-angiogenic factor vascular endothelial growth factor (VEGF) was found to be especially expressed by neurones of ganglion cell layer, both in control and in MTC-injected eyes. The data obtained in this experimental model on the interactions among glia, vessels and neurons should be useful to evaluate if also in glaucomatous patients the activation of vessel-adjacent glial cells might play key roles in following neuronal dysfunction. |
| first_indexed | 2024-12-12T21:11:51Z |
| format | Article |
| id | doaj.art-146004062c83423ba182c16508eeb6ae |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-12T21:11:51Z |
| publishDate | 2012-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-146004062c83423ba182c16508eeb6ae2022-12-22T00:11:52ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062012-10-01564e42e4210.4081/ejh.2012.e421295Degenerative effects in rat eyes after experimental ocular hypertensionG. Scarsella0M. Nebbioso1S. StefaniniN. Pescosolido2University Sapienza of RomeUniversity Sapienza of RomeUniversity Sapienza of RomeThis study was used to evaluate the degenerative effects on the retina and eye-cup sections after experimental induction of acute ocular hypertension on animal models. In particular, vascular events were directly focused in this research in order to assess the vascular remodeling after transient ocular hypertension on rat models. After local anaesthesia by administration of eye drops of 0.4% oxibuprocaine, 16 male adult <em>Wistar</em> rats were injected in the anterior chamber of the right eye with 15 µL of methylcellulose (MTC) 2% in physiological solution. The morphology and the vessels of the retina and eye-cup sections were examined in animals sacrificed 72 h after induction of ocular hypertension. In retinal fluorescein angiographies (FAGs), by means of fluorescein isothiocyanate-coniugated dextran (FITC), the radial venules showed enlargements and increased branching, while the arterioles appeared focally thickened. The length and size of actually perfused vessels appeared increased in the whole superficial plexus. In eye-cup sections of MTC-injected animals, in deep plexus and connecting layer there was a bigger increase of vessels than in controls. Moreover, the immunolocalization of astrocytic marker glial fibrillary acidic protein (GFAP) revealed its increased expression in internal limiting membrane and ganglion cell layer, as well as its presence in Müller cells. Finally, the pro-angiogenic factor vascular endothelial growth factor (VEGF) was found to be especially expressed by neurones of ganglion cell layer, both in control and in MTC-injected eyes. The data obtained in this experimental model on the interactions among glia, vessels and neurons should be useful to evaluate if also in glaucomatous patients the activation of vessel-adjacent glial cells might play key roles in following neuronal dysfunction.http://www.ejh.it/index.php/ejh/article/view/2068angiogenesis, Bandeira simplicifolia isolectin B4 labelling, Glaucoma, Glial fibrillary acidic protein immunolocalization, VEGF immunolocalization |
| spellingShingle | G. Scarsella M. Nebbioso S. Stefanini N. Pescosolido Degenerative effects in rat eyes after experimental ocular hypertension European Journal of Histochemistry angiogenesis, Bandeira simplicifolia isolectin B4 labelling, Glaucoma, Glial fibrillary acidic protein immunolocalization, VEGF immunolocalization |
| title | Degenerative effects in rat eyes after experimental ocular hypertension |
| title_full | Degenerative effects in rat eyes after experimental ocular hypertension |
| title_fullStr | Degenerative effects in rat eyes after experimental ocular hypertension |
| title_full_unstemmed | Degenerative effects in rat eyes after experimental ocular hypertension |
| title_short | Degenerative effects in rat eyes after experimental ocular hypertension |
| title_sort | degenerative effects in rat eyes after experimental ocular hypertension |
| topic | angiogenesis, Bandeira simplicifolia isolectin B4 labelling, Glaucoma, Glial fibrillary acidic protein immunolocalization, VEGF immunolocalization |
| url | http://www.ejh.it/index.php/ejh/article/view/2068 |
| work_keys_str_mv | AT gscarsella degenerativeeffectsinrateyesafterexperimentalocularhypertension AT mnebbioso degenerativeeffectsinrateyesafterexperimentalocularhypertension AT sstefanini degenerativeeffectsinrateyesafterexperimentalocularhypertension AT npescosolido degenerativeeffectsinrateyesafterexperimentalocularhypertension |