In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
<i>Olea europaea</i> L. var. <i>sativa</i> (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxi...
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MDPI AG
2021-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/11/2445 |
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| author | Ichrak Ben-Amor Bochra Gargouri Hamadi Attia Khaoula Tlili Imen Kallel Maria Musarra-Pizzo Maria Teresa Sciortino Rosamaria Pennisi |
| author_facet | Ichrak Ben-Amor Bochra Gargouri Hamadi Attia Khaoula Tlili Imen Kallel Maria Musarra-Pizzo Maria Teresa Sciortino Rosamaria Pennisi |
| author_sort | Ichrak Ben-Amor |
| collection | DOAJ |
| description | <i>Olea europaea</i> L. var. <i>sativa</i> (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC<sub>50</sub> values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (<i>p</i> < 0.05). Besides, Raji cells simultaneously exposed to TPA and OESA exhibited a percentage of EBV-positive fluorescence cells lower than TPA treated cells (**** <i>p</i> < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction. |
| first_indexed | 2024-03-10T05:07:36Z |
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| id | doaj.art-14623be7920b4ce5bf74dcbbcf4e0002 |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T05:07:36Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-14623be7920b4ce5bf74dcbbcf4e00022023-11-23T01:05:51ZengMDPI AGPlants2223-77472021-11-011011244510.3390/plants10112445In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf ExtractsIchrak Ben-Amor0Bochra Gargouri1Hamadi Attia2Khaoula Tlili3Imen Kallel4Maria Musarra-Pizzo5Maria Teresa Sciortino6Rosamaria Pennisi7Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, ItalyUnit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, TunisiaUnit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, TunisiaUnit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, TunisiaLaboratoire de Recherche Toxicologie-Microbiologie Environnementale et Santé, Faculté des Sciences de Sfax, Sfax 3000, TunisiaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno Alcontres, 31, 98166 Messina, Italy<i>Olea europaea</i> L. var. <i>sativa</i> (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC<sub>50</sub> values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (<i>p</i> < 0.05). Besides, Raji cells simultaneously exposed to TPA and OESA exhibited a percentage of EBV-positive fluorescence cells lower than TPA treated cells (**** <i>p</i> < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction.https://www.mdpi.com/2223-7747/10/11/2445<i>Olea europaea</i> leavesantiviralsantioxidantplants bioactive compounds |
| spellingShingle | Ichrak Ben-Amor Bochra Gargouri Hamadi Attia Khaoula Tlili Imen Kallel Maria Musarra-Pizzo Maria Teresa Sciortino Rosamaria Pennisi In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts Plants <i>Olea europaea</i> leaves antivirals antioxidant plants bioactive compounds |
| title | In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts |
| title_full | In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts |
| title_fullStr | In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts |
| title_full_unstemmed | In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts |
| title_short | In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts |
| title_sort | in vitro anti epstein barr virus activity of i olea europaea i l leaf extracts |
| topic | <i>Olea europaea</i> leaves antivirals antioxidant plants bioactive compounds |
| url | https://www.mdpi.com/2223-7747/10/11/2445 |
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