Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region
<p>Abstract</p> <p>Background</p> <p><it>Euphorbia mosaic virus </it>(EuMV) is a member of the SLCV clade, a lineage of New World begomoviruses that display distinctive features in their replication-associated protein (Rep) and virion-strand replication orig...
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BMC
2010-10-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/7/1/275 |
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| author | Argüello-Astorga Gerardo R Frías-Treviño Gustavo Moreno-Valenzuela Oscar Hernández-Zepeda Cecilia Alpuche-Solís Ángel G Bañuelos-Hernández Bernardo Bernal-Alcocer Artemiza Gregorio-Jorge Josefat |
| author_facet | Argüello-Astorga Gerardo R Frías-Treviño Gustavo Moreno-Valenzuela Oscar Hernández-Zepeda Cecilia Alpuche-Solís Ángel G Bañuelos-Hernández Bernardo Bernal-Alcocer Artemiza Gregorio-Jorge Josefat |
| author_sort | Argüello-Astorga Gerardo R |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Euphorbia mosaic virus </it>(EuMV) is a member of the SLCV clade, a lineage of New World begomoviruses that display distinctive features in their replication-associated protein (Rep) and virion-strand replication origin. The first entirely characterized EuMV isolate is native from Yucatan Peninsula, Mexico; subsequently, EuMV was detected in weeds and pepper plants from another region of Mexico, and partial DNA-A sequences revealed significant differences in their putative replication specificity determinants with respect to EuMV-YP. This study was aimed to investigate the replication compatibility between two EuMV isolates from the same country.</p> <p>Results</p> <p>A new isolate of EuMV was obtained from pepper plants collected at Jalisco, Mexico. Full-length clones of both genomic components of EuMV-Jal were biolistically inoculated into plants of three different species, which developed symptoms indistinguishable from those induced by EuMV-YP. Pseudorecombination experiments with EuMV-Jal and EuMV-YP genomic components demonstrated that these viruses do not form infectious reassortants in <it>Nicotiana benthamiana</it>, presumably because of Rep-iteron incompatibility. Sequence analysis of the EuMV-Jal DNA-B intergenic region (IR) led to the unexpected discovery of a 35-nt-long sequence that is identical to a segment of the <it>rep </it>gene in the cognate viral DNA-A. Similar short <it>rep </it>sequences ranging from 35- to 51-nt in length were identified in all EuMV isolates and in three distinct viruses from South America related to EuMV. These short <it>rep </it>sequences in the DNA-B IR are positioned downstream to a ~160-nt non-coding domain highly similar to the CP promoter of begomoviruses belonging to the SLCV clade.</p> <p>Conclusions</p> <p>EuMV strains are not compatible in replication, indicating that this begomovirus species probably is not a replicating lineage in nature. The genomic analysis of EuMV-Jal led to the discovery of a subgroup of SLCV clade viruses that contain in the non-coding region of their DNA-B component, short <it>rep </it>gene sequences located downstream to a <it>CP</it>-promoter-like domain. This assemblage of DNA-A-related sequences within the DNA-B IR is reminiscent of polyomavirus microRNAs and could be involved in the posttranscriptional regulation of the cognate viral <it>rep </it>gene, an intriguing possibility that should be experimentally explored</p> |
| first_indexed | 2024-04-12T15:29:55Z |
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| language | English |
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| publishDate | 2010-10-01 |
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| spelling | doaj.art-1467cef139c5447ca7b2410d809817e62022-12-22T03:27:09ZengBMCVirology Journal1743-422X2010-10-017127510.1186/1743-422X-7-275Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic regionArgüello-Astorga Gerardo RFrías-Treviño GustavoMoreno-Valenzuela OscarHernández-Zepeda CeciliaAlpuche-Solís Ángel GBañuelos-Hernández BernardoBernal-Alcocer ArtemizaGregorio-Jorge Josefat<p>Abstract</p> <p>Background</p> <p><it>Euphorbia mosaic virus </it>(EuMV) is a member of the SLCV clade, a lineage of New World begomoviruses that display distinctive features in their replication-associated protein (Rep) and virion-strand replication origin. The first entirely characterized EuMV isolate is native from Yucatan Peninsula, Mexico; subsequently, EuMV was detected in weeds and pepper plants from another region of Mexico, and partial DNA-A sequences revealed significant differences in their putative replication specificity determinants with respect to EuMV-YP. This study was aimed to investigate the replication compatibility between two EuMV isolates from the same country.</p> <p>Results</p> <p>A new isolate of EuMV was obtained from pepper plants collected at Jalisco, Mexico. Full-length clones of both genomic components of EuMV-Jal were biolistically inoculated into plants of three different species, which developed symptoms indistinguishable from those induced by EuMV-YP. Pseudorecombination experiments with EuMV-Jal and EuMV-YP genomic components demonstrated that these viruses do not form infectious reassortants in <it>Nicotiana benthamiana</it>, presumably because of Rep-iteron incompatibility. Sequence analysis of the EuMV-Jal DNA-B intergenic region (IR) led to the unexpected discovery of a 35-nt-long sequence that is identical to a segment of the <it>rep </it>gene in the cognate viral DNA-A. Similar short <it>rep </it>sequences ranging from 35- to 51-nt in length were identified in all EuMV isolates and in three distinct viruses from South America related to EuMV. These short <it>rep </it>sequences in the DNA-B IR are positioned downstream to a ~160-nt non-coding domain highly similar to the CP promoter of begomoviruses belonging to the SLCV clade.</p> <p>Conclusions</p> <p>EuMV strains are not compatible in replication, indicating that this begomovirus species probably is not a replicating lineage in nature. The genomic analysis of EuMV-Jal led to the discovery of a subgroup of SLCV clade viruses that contain in the non-coding region of their DNA-B component, short <it>rep </it>gene sequences located downstream to a <it>CP</it>-promoter-like domain. This assemblage of DNA-A-related sequences within the DNA-B IR is reminiscent of polyomavirus microRNAs and could be involved in the posttranscriptional regulation of the cognate viral <it>rep </it>gene, an intriguing possibility that should be experimentally explored</p>http://www.virologyj.com/content/7/1/275 |
| spellingShingle | Argüello-Astorga Gerardo R Frías-Treviño Gustavo Moreno-Valenzuela Oscar Hernández-Zepeda Cecilia Alpuche-Solís Ángel G Bañuelos-Hernández Bernardo Bernal-Alcocer Artemiza Gregorio-Jorge Josefat Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region Virology Journal |
| title | Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region |
| title_full | Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region |
| title_fullStr | Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region |
| title_full_unstemmed | Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region |
| title_short | Analysis of a new strain of <it>Euphorbia mosaic virus </it>with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region |
| title_sort | analysis of a new strain of it euphorbia mosaic virus it with distinct replication specificity unveils a lineage of begomoviruses with short rep sequences in the dna b intergenic region |
| url | http://www.virologyj.com/content/7/1/275 |
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