Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.)
Mitogen-activated protein kinase (MAPK) is an important component of the signal transduction pathway, which plays important roles in regulating plant growth and development, and abiotic stress. Potato (<i>Solanum tuberosum</i> L.) is one of the most popular tuber crops in the world. Geno...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/1/93 |
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| author | Yutong Shang Xiaobo Luo Heng Zhang Mingjun Chen Wang Yin Zhenju Cao Renju Deng Yan Li Fei Li |
| author_facet | Yutong Shang Xiaobo Luo Heng Zhang Mingjun Chen Wang Yin Zhenju Cao Renju Deng Yan Li Fei Li |
| author_sort | Yutong Shang |
| collection | DOAJ |
| description | Mitogen-activated protein kinase (MAPK) is an important component of the signal transduction pathway, which plays important roles in regulating plant growth and development, and abiotic stress. Potato (<i>Solanum tuberosum</i> L.) is one of the most popular tuber crops in the world. Genome-wide identification and analysis of the <i>MAPK</i> and <i>MAPKK</i> gene family in potato is not clear. A total of 20 <i>MAPK</i> genes and 8 <i>MAPKK</i> genes were identified in the potato genome. A conservative motif analysis showed that the MAPK protein contained a typical TxY phosphorylation site, and the MAPKK protein contained a conservative characteristic motif S/T-x5-S/T. Phylogenetic analysis showed that potato <i>MAPK</i> (mitogen-activated protein kinase) and <i>MAPKK</i> (mitogen-activated protein kinase kinase) were similar to Arabidopsis, including four groups of members A, B, C and D. Gene structure and promoter sequence analysis showed that all 28 gene family members of potato <i>Solanum tuberosum MAPK</i> (<i>StMAPK</i>) and <i>StMAPKK</i> have coding regions (CDS), and family members in the same group have similar intron and exon compositions, and that most cis-acting elements upstream of gene promoters elements have related to stress response. Chromosome location analysis found that MAPKs were unevenly distributed on 11 chromosomes, while MAPKKs were only distributed on chromosomes Chr. 03 and Chr. 12. Collinearity analysis showed that <i>StMAPKK3</i> and <i>StMAPKK6</i> have the same common ancestors among potato, pepper, and tomato. qRT-PCR results showed that the relative expressions of <i>StMAPK14</i> and <i>StMAPKK2</i> were significantly upregulated under low-temperature stress. These results could provide new insights into the characteristics and evolution of the <i>StMAPK</i> and <i>StMAPKK</i> gene family and facilitate further exploration of the molecular mechanism responsible for potato abiotic stress responses. |
| first_indexed | 2024-03-09T13:51:46Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:51:46Z |
| publishDate | 2022-12-01 |
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| series | Agronomy |
| spelling | doaj.art-1469761b4f1640e9ad4dc435a8452a4a2023-11-30T20:48:50ZengMDPI AGAgronomy2073-43952022-12-011319310.3390/agronomy13010093Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.)Yutong Shang0Xiaobo Luo1Heng Zhang2Mingjun Chen3Wang Yin4Zhenju Cao5Renju Deng6Yan Li7Fei Li8Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaGuizhou Institute of Biotechnology, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550003, ChinaGuizhou Institute of Biotechnology, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550003, ChinaGuizhou Institute of Biotechnology, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550003, ChinaGuizhou Institute of Biotechnology, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550003, ChinaGuizhou Institute of Biotechnology, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550003, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaGuizhou Institute of Biotechnology, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550003, ChinaMitogen-activated protein kinase (MAPK) is an important component of the signal transduction pathway, which plays important roles in regulating plant growth and development, and abiotic stress. Potato (<i>Solanum tuberosum</i> L.) is one of the most popular tuber crops in the world. Genome-wide identification and analysis of the <i>MAPK</i> and <i>MAPKK</i> gene family in potato is not clear. A total of 20 <i>MAPK</i> genes and 8 <i>MAPKK</i> genes were identified in the potato genome. A conservative motif analysis showed that the MAPK protein contained a typical TxY phosphorylation site, and the MAPKK protein contained a conservative characteristic motif S/T-x5-S/T. Phylogenetic analysis showed that potato <i>MAPK</i> (mitogen-activated protein kinase) and <i>MAPKK</i> (mitogen-activated protein kinase kinase) were similar to Arabidopsis, including four groups of members A, B, C and D. Gene structure and promoter sequence analysis showed that all 28 gene family members of potato <i>Solanum tuberosum MAPK</i> (<i>StMAPK</i>) and <i>StMAPKK</i> have coding regions (CDS), and family members in the same group have similar intron and exon compositions, and that most cis-acting elements upstream of gene promoters elements have related to stress response. Chromosome location analysis found that MAPKs were unevenly distributed on 11 chromosomes, while MAPKKs were only distributed on chromosomes Chr. 03 and Chr. 12. Collinearity analysis showed that <i>StMAPKK3</i> and <i>StMAPKK6</i> have the same common ancestors among potato, pepper, and tomato. qRT-PCR results showed that the relative expressions of <i>StMAPK14</i> and <i>StMAPKK2</i> were significantly upregulated under low-temperature stress. These results could provide new insights into the characteristics and evolution of the <i>StMAPK</i> and <i>StMAPKK</i> gene family and facilitate further exploration of the molecular mechanism responsible for potato abiotic stress responses.https://www.mdpi.com/2073-4395/13/1/93potatoMAPKMAPKKgene familylow-temperature stress |
| spellingShingle | Yutong Shang Xiaobo Luo Heng Zhang Mingjun Chen Wang Yin Zhenju Cao Renju Deng Yan Li Fei Li Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.) Agronomy potato MAPK MAPKK gene family low-temperature stress |
| title | Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.) |
| title_full | Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.) |
| title_fullStr | Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.) |
| title_full_unstemmed | Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.) |
| title_short | Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (<i>Solanum tuberosum</i> L.) |
| title_sort | genome wide identification and analysis of the mapk and mapkk gene families in potato i solanum tuberosum i l |
| topic | potato MAPK MAPKK gene family low-temperature stress |
| url | https://www.mdpi.com/2073-4395/13/1/93 |
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