Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy

Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy

Summary: FACS sorting followed by single-cell RNA-sequencing (SORT-Seq) is a popular procedure to select cells of interest for single-cell transcriptomics. However, FACS is not suitable for measurement of subcellular distribution of fluorescence or for small samples (<1,000 cells). The VYCAP punc...

Full description

Bibliographic Details
Main Authors: Hendrika A. Segeren, Kiki C. Andree, Lisa Oomens, Bart Westendorp
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Single Cell
Genomics
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004251

Similar Items

Similar Items