An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients
Abstract Conventional PD‐L1 immunohistochemical tissue biopsies only predict 20%–40% of non‐small cell lung cancer (NSCLC) patients that will respond positively to anti‐PD‐1/PD‐L1 immunotherapy. Herein, we present an immunogold biochip to quantify single extracellular vesicular RNA and protein (AuSE...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-09-01
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| Series: | Journal of Extracellular Vesicles |
| Online Access: | https://doi.org/10.1002/jev2.12258 |
| _version_ | 1811259019187191808 |
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| author | Luong T. H. Nguyen Jingjing Zhang Xilal Y. Rima Xinyu Wang Kwang Joo Kwak Tamio Okimoto Joseph Amann Min Jin Yoon Takehito Shukuya Chi‐Ling Chiang Nicole Walters Yifan Ma Donald Belcher Hong Li Andre F. Palmer David P. Carbone L. James Lee Eduardo Reátegui |
| author_facet | Luong T. H. Nguyen Jingjing Zhang Xilal Y. Rima Xinyu Wang Kwang Joo Kwak Tamio Okimoto Joseph Amann Min Jin Yoon Takehito Shukuya Chi‐Ling Chiang Nicole Walters Yifan Ma Donald Belcher Hong Li Andre F. Palmer David P. Carbone L. James Lee Eduardo Reátegui |
| author_sort | Luong T. H. Nguyen |
| collection | DOAJ |
| description | Abstract Conventional PD‐L1 immunohistochemical tissue biopsies only predict 20%–40% of non‐small cell lung cancer (NSCLC) patients that will respond positively to anti‐PD‐1/PD‐L1 immunotherapy. Herein, we present an immunogold biochip to quantify single extracellular vesicular RNA and protein (AuSERP) as a non‐invasive alternative. With only 20 μl of purified serum, PD‐1/PD‐L1 proteins on the surface of extracellular vesicles (EVs) and EV PD‐1/PD‐L1 messenger RNA (mRNA) cargo were detected at a single‐vesicle resolution and exceeded the sensitivities of their bulk‐analysis conventional counterparts, ELISA and qRT‐PCR, by 1000 times. By testing a cohort of 27 non‐responding and 27 responding NSCLC patients, AuSERP indicated that the single‐EV mRNA biomarkers surpass the single‐EV protein biomarkers in predicting patient responses to immunotherapy. Dual single‐EV PD‐1/PD‐L1 mRNA detection differentiated responders from non‐responders with an accuracy of 72.2% and achieved an NSCLC diagnosis accuracy of 93.2%, suggesting the potential for AuSERP to provide enhanced immunotherapy predictions and cancer diagnoses within the clinical setting. |
| first_indexed | 2024-04-12T18:23:16Z |
| format | Article |
| id | doaj.art-146b93eb927b403f95099c23ef145b70 |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-04-12T18:23:16Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-146b93eb927b403f95099c23ef145b702022-12-22T03:21:19ZengWileyJournal of Extracellular Vesicles2001-30782022-09-01119n/an/a10.1002/jev2.12258An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patientsLuong T. H. Nguyen0Jingjing Zhang1Xilal Y. Rima2Xinyu Wang3Kwang Joo Kwak4Tamio Okimoto5Joseph Amann6Min Jin Yoon7Takehito Shukuya8Chi‐Ling Chiang9Nicole Walters10Yifan Ma11Donald Belcher12Hong Li13Andre F. Palmer14David P. Carbone15L. James Lee16Eduardo Reátegui17William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USASpot Biosystems Ltd. Palo Alto California USAComprehensive Cancer Center The Ohio State University Columbus Ohio USAComprehensive Cancer Center The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAComprehensive Cancer Center The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAComprehensive Cancer Center The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAWilliam G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USAAbstract Conventional PD‐L1 immunohistochemical tissue biopsies only predict 20%–40% of non‐small cell lung cancer (NSCLC) patients that will respond positively to anti‐PD‐1/PD‐L1 immunotherapy. Herein, we present an immunogold biochip to quantify single extracellular vesicular RNA and protein (AuSERP) as a non‐invasive alternative. With only 20 μl of purified serum, PD‐1/PD‐L1 proteins on the surface of extracellular vesicles (EVs) and EV PD‐1/PD‐L1 messenger RNA (mRNA) cargo were detected at a single‐vesicle resolution and exceeded the sensitivities of their bulk‐analysis conventional counterparts, ELISA and qRT‐PCR, by 1000 times. By testing a cohort of 27 non‐responding and 27 responding NSCLC patients, AuSERP indicated that the single‐EV mRNA biomarkers surpass the single‐EV protein biomarkers in predicting patient responses to immunotherapy. Dual single‐EV PD‐1/PD‐L1 mRNA detection differentiated responders from non‐responders with an accuracy of 72.2% and achieved an NSCLC diagnosis accuracy of 93.2%, suggesting the potential for AuSERP to provide enhanced immunotherapy predictions and cancer diagnoses within the clinical setting.https://doi.org/10.1002/jev2.12258 |
| spellingShingle | Luong T. H. Nguyen Jingjing Zhang Xilal Y. Rima Xinyu Wang Kwang Joo Kwak Tamio Okimoto Joseph Amann Min Jin Yoon Takehito Shukuya Chi‐Ling Chiang Nicole Walters Yifan Ma Donald Belcher Hong Li Andre F. Palmer David P. Carbone L. James Lee Eduardo Reátegui An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients Journal of Extracellular Vesicles |
| title | An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients |
| title_full | An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients |
| title_fullStr | An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients |
| title_full_unstemmed | An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients |
| title_short | An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients |
| title_sort | immunogold single extracellular vesicular rna and protein auserp biochip to predict responses to immunotherapy in non small cell lung cancer patients |
| url | https://doi.org/10.1002/jev2.12258 |
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