On-slide tissue digestion for mass spectrometry based glycomic and proteomic profiling

On-slide tissue digestion for mass spectrometry based glycomic and proteomic profiling

We describe a protocol for glycomic and proteomic profiling that uses serial enzyme digestions from the surface of fresh frozen or fixed tissue slides. The abundances of the extracted glycans and peptides are determined using liquid chromatography-mass spectrometry. In a typical experiment, our meth...

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Bibliographic Details
Main Authors:	Rekha Raghunathan, Manveen K. Sethi, Joseph Zaia
Format:	Article
Language:	English
Published:	Elsevier 2019-01-01
Series:	MethodsX
Online Access:	http://www.sciencedirect.com/science/article/pii/S2215016119302535

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