Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood
The present study was designed to investigate the effects of storage temperature and time on blood gas and acid-base balance of ovine venous blood. Ten clinically healthy sheep were used in this study. A total number of 30 blood samples, were divided into three different groups, and were stored in a...
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| Format: | Article |
| Language: | English |
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Tripoli University
2013-01-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.openveterinaryjournal.com/2013/Volume%203%20(1)/OVJ-098-08-12%20H.A.%20Hussein%20and%20A.A.%20Aamer.pdf |
| _version_ | 1818569773118652416 |
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| author | H.A. Hussein A.A. Aamer |
| author_facet | H.A. Hussein A.A. Aamer |
| author_sort | H.A. Hussein |
| collection | DOAJ |
| description | The present study was designed to investigate the effects of storage temperature and time on blood gas and acid-base balance of ovine venous blood. Ten clinically healthy sheep were used in this study. A total number of 30 blood samples, were divided into three different groups, and were stored in a refrigerator adjusted to +4 ºC (Group I, n = 10), at RT of about 22-25 ºC (Group II, n = 10) and in an incubator adjusted to 37 ºC (Group III, n = 10) for up to 48 h. Blood samples were analysed for blood gas and acid-base indices at 0, 1, 2, 3, 4, 5, 6, 12, 24 and 48 h of storage. In comparison to the baseline value (0), there were significant decreases of blood pH of samples stored at RT and in the incubator after 1 h (p<0.05), the pH value of refrigerated blood samples exhibited insignificant changes during the study (p<0.05). Mean values of pCO2 showed a significant increase in Group I and Group III after 1 h then a progressive decrease after 12 h in all Groups. Mean pO2 values were significantly higher for Group I after 2 h and for Groups II and III after 1 h (p<0.05). In general, base excess decreased significantly for all the groups during the study especially in Groups II and III. In comparison with baseline values, in all groups, bicarbonate (HCO3) increased between 1 h and 6 h (p<0.05), and later decreased at the end of the study (p<0.05). In conclusion, status of acid-base indices of the samples stored at refrigerator and RT were found within normal reference range and it may be of clinical diagnostic use for up to 6 h. |
| first_indexed | 2024-12-14T06:52:07Z |
| format | Article |
| id | doaj.art-14718b12cad243f69bd4b9a95b174ae2 |
| institution | Directory Open Access Journal |
| issn | 2218-6050 |
| language | English |
| last_indexed | 2024-12-14T06:52:07Z |
| publishDate | 2013-01-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-14718b12cad243f69bd4b9a95b174ae22022-12-21T23:12:52ZengTripoli UniversityOpen Veterinary Journal2218-60502013-01-013117Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous bloodH.A. HusseinA.A. AamerThe present study was designed to investigate the effects of storage temperature and time on blood gas and acid-base balance of ovine venous blood. Ten clinically healthy sheep were used in this study. A total number of 30 blood samples, were divided into three different groups, and were stored in a refrigerator adjusted to +4 ºC (Group I, n = 10), at RT of about 22-25 ºC (Group II, n = 10) and in an incubator adjusted to 37 ºC (Group III, n = 10) for up to 48 h. Blood samples were analysed for blood gas and acid-base indices at 0, 1, 2, 3, 4, 5, 6, 12, 24 and 48 h of storage. In comparison to the baseline value (0), there were significant decreases of blood pH of samples stored at RT and in the incubator after 1 h (p<0.05), the pH value of refrigerated blood samples exhibited insignificant changes during the study (p<0.05). Mean values of pCO2 showed a significant increase in Group I and Group III after 1 h then a progressive decrease after 12 h in all Groups. Mean pO2 values were significantly higher for Group I after 2 h and for Groups II and III after 1 h (p<0.05). In general, base excess decreased significantly for all the groups during the study especially in Groups II and III. In comparison with baseline values, in all groups, bicarbonate (HCO3) increased between 1 h and 6 h (p<0.05), and later decreased at the end of the study (p<0.05). In conclusion, status of acid-base indices of the samples stored at refrigerator and RT were found within normal reference range and it may be of clinical diagnostic use for up to 6 h.http://www.openveterinaryjournal.com/2013/Volume%203%20(1)/OVJ-098-08-12%20H.A.%20Hussein%20and%20A.A.%20Aamer.pdfAcid-base indicesClinical relevanceOvineStorage temperature time. |
| spellingShingle | H.A. Hussein A.A. Aamer Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood Open Veterinary Journal Acid-base indices Clinical relevance Ovine Storage temperature time. |
| title | Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood |
| title_full | Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood |
| title_fullStr | Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood |
| title_full_unstemmed | Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood |
| title_short | Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood |
| title_sort | influence of different storage times and temperatures on blood gas and acid base balance in ovine venous blood |
| topic | Acid-base indices Clinical relevance Ovine Storage temperature time. |
| url | http://www.openveterinaryjournal.com/2013/Volume%203%20(1)/OVJ-098-08-12%20H.A.%20Hussein%20and%20A.A.%20Aamer.pdf |
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