Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics
Introduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro ex...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2021-04-01
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| Series: | Foods and Raw Materials |
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| Online Access: | http://jfrm.ru/files/archive/17/Milentyeva.pdf |
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| author | Irina S. Milentyeva Violeta M. Le Oksana V. Kozlova Natalia S. Velichkovich Anastasiya M. Fedorova Anna I. Loseva Vladimir P. Yustratov |
| author_facet | Irina S. Milentyeva Violeta M. Le Oksana V. Kozlova Natalia S. Velichkovich Anastasiya M. Fedorova Anna I. Loseva Vladimir P. Yustratov |
| author_sort | Irina S. Milentyeva |
| collection | DOAJ |
| description | Introduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro extracts of wild Siberian plants.
Study objects and methods. The study featured callus, cell suspension, and hairy root extracts of such Siberian medicinal plants as Eleutherococcus senticosus, Codonopsis pilosula, Platanthera bifolia, and Saposhnikovia divaricata. They were obtained by in vitro cultivation using modified nutrient media of Murashige and Skoog and Gamborg. The content of secondary metabolites was studied using the methods of thin-layer and high-performance liquid chromatography. A set of in vitro experiments tested the antioxidant and antimicrobial activity of the extracts.
Results and discussion. All the samples demonstrated a high content of secondary metabolites of phenolic nature. Flavonoglycosides, apigenin, and rutin were found to be the predominant biologically active substances in the callus extracts. Flavonoglycosides dominated in the suspension extracts. The root extracts contained more caffeic acid, rutin, ecdysteroids, quercetin, apigenin, cardiofolin, and coleofolide than the callus and suspension cultures. The list of prevailing secondary metabolites in the root extracts included rutin, apigenin, coleofolide, and quercetin. All the extracts showed antimicrobial and antioxidant activity.
Conclusion. All the extracts demonstrated good antioxidant and antimicrobial properties. Therefore, they can be used for the production of pharmaceuticals and biologically active food supplements as they can be helpful against infectious diseases, as well as oncological, cardiovascular, and neurodegenerative diseases linked to oxidative stress. |
| first_indexed | 2024-12-14T23:12:16Z |
| format | Article |
| id | doaj.art-14722654a2ba47c58c38d655375252f5 |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-12-14T23:12:16Z |
| publishDate | 2021-04-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-14722654a2ba47c58c38d655375252f52022-12-21T22:44:10ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992021-04-019115316310.21603/2308-4057-2021-1-153-163Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristicsIrina S. Milentyeva0https://orcid.org/0000-0002-3536-562XVioleta M. Le1https://orcid.org/0000-0002-9546-6633Oksana V. Kozlova2https://orcid.org/0000-0002-2960-0216Natalia S. Velichkovich3https://orcid.org/0000-0002-9061-1256Anastasiya M. Fedorova4https://orcid.org/0000-0002-8071-4411Anna I. Loseva5https://orcid.org/0000-0003-4037-2653Vladimir P. Yustratov6Kemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaIntroduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro extracts of wild Siberian plants. Study objects and methods. The study featured callus, cell suspension, and hairy root extracts of such Siberian medicinal plants as Eleutherococcus senticosus, Codonopsis pilosula, Platanthera bifolia, and Saposhnikovia divaricata. They were obtained by in vitro cultivation using modified nutrient media of Murashige and Skoog and Gamborg. The content of secondary metabolites was studied using the methods of thin-layer and high-performance liquid chromatography. A set of in vitro experiments tested the antioxidant and antimicrobial activity of the extracts. Results and discussion. All the samples demonstrated a high content of secondary metabolites of phenolic nature. Flavonoglycosides, apigenin, and rutin were found to be the predominant biologically active substances in the callus extracts. Flavonoglycosides dominated in the suspension extracts. The root extracts contained more caffeic acid, rutin, ecdysteroids, quercetin, apigenin, cardiofolin, and coleofolide than the callus and suspension cultures. The list of prevailing secondary metabolites in the root extracts included rutin, apigenin, coleofolide, and quercetin. All the extracts showed antimicrobial and antioxidant activity. Conclusion. All the extracts demonstrated good antioxidant and antimicrobial properties. Therefore, they can be used for the production of pharmaceuticals and biologically active food supplements as they can be helpful against infectious diseases, as well as oncological, cardiovascular, and neurodegenerative diseases linked to oxidative stress.http://jfrm.ru/files/archive/17/Milentyeva.pdfcallus culturecell suspension culturehairy rootsmedicinal plantsecondary metabolitephenolic substancesantioxidantoxidative stressantimicrobial propertiesextraction |
| spellingShingle | Irina S. Milentyeva Violeta M. Le Oksana V. Kozlova Natalia S. Velichkovich Anastasiya M. Fedorova Anna I. Loseva Vladimir P. Yustratov Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics Foods and Raw Materials callus culture cell suspension culture hairy roots medicinal plant secondary metabolite phenolic substances antioxidant oxidative stress antimicrobial properties extraction |
| title | Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics |
| title_full | Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics |
| title_fullStr | Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics |
| title_full_unstemmed | Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics |
| title_short | Secondary metabolites in in vitro cultures of Siberian medicinal plants: Content, antioxidant properties, and antimicrobial characteristics |
| title_sort | secondary metabolites in in vitro cultures of siberian medicinal plants content antioxidant properties and antimicrobial characteristics |
| topic | callus culture cell suspension culture hairy roots medicinal plant secondary metabolite phenolic substances antioxidant oxidative stress antimicrobial properties extraction |
| url | http://jfrm.ru/files/archive/17/Milentyeva.pdf |
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