The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells
Objective:Green tea (Camellia sinensis) is one of the most consumed beverages in the world. C. sinensis consumption may prevent tumor and other diseases. Insulinomas are pancreatic islet cell tumors. In present study, the effects of the extract from C. sinensis were investigated in rat insulinoma IN...
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Galenos Publishing House
2019-04-01
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| Series: | Bezmiâlem Science |
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http://bezmialemscience.org/archives/archive-detail/article-preview/the-effects-of-icamellia-sinensis-i-extract-on-pro/20608
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| author | Ayşe Karatuğ KAÇAR Melike ERSÖZ Işıl SEZEKLER Zeynep Mine COŞKUN |
| author_facet | Ayşe Karatuğ KAÇAR Melike ERSÖZ Işıl SEZEKLER Zeynep Mine COŞKUN |
| author_sort | Ayşe Karatuğ KAÇAR |
| collection | DOAJ |
| description | Objective:Green tea (Camellia sinensis) is one of the most consumed beverages in the world. C. sinensis consumption may prevent tumor and other diseases. Insulinomas are pancreatic islet cell tumors. In present study, the effects of the extract from C. sinensis were investigated in rat insulinoma INS-1 cells.Methods:C. sinensis leaves were collected in Rize, Turkey. The leaves were brewed in tap water for 20 and 40 minutes at 80°C. INS-1 cells were treated with different doses (1-5 mg/mL) of C. sinensis extract for 24 h. Cell viability, proliferation, death were examined. The malondialdehyde, glutathione and protein carbonyl levels were measured in INS-1 cells given C. sinensis extractResults:The cell viability and the percentage of proliferating cell nuclear antigen immunopositive cells decreased, while the percentage of TUNEL positive cells increased in INS-1 cells treated with the extract from C. sinensis according to control group. While malondialdehyde and protein carbonyl levels in INS-1 cells treated with C. sinensis extract decreased, glutathione levels increased according to control group.Conclusion:The results indicate that the extracts from C. sinensis inhibited proliferation and caused apoptosis in INS-1 cell. C. sinensis may be natural agent for supporting the treatment of pancreatic tumors. |
| first_indexed | 2024-04-10T11:15:19Z |
| format | Article |
| id | doaj.art-14745c39401545fabd5f6eeca8655990 |
| institution | Directory Open Access Journal |
| issn | 2148-2373 2148-2373 |
| language | English |
| last_indexed | 2024-04-10T11:15:19Z |
| publishDate | 2019-04-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Bezmiâlem Science |
| spelling | doaj.art-14745c39401545fabd5f6eeca86559902023-02-15T16:18:53ZengGalenos Publishing HouseBezmiâlem Science2148-23732148-23732019-04-0172808510.14235/bas.galenos.2018.228713049054The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 CellsAyşe Karatuğ KAÇAR0Melike ERSÖZ1Işıl SEZEKLER2Zeynep Mine COŞKUN3 İstanbul University, Faculty of Science, Department of Biology, Istanbul, Turkey Demiroğlu Bilim University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, İstanbul, Turkey Marmara University, Faculty of Science, Department of Biology, İstanbul, Turkey Demiroğlu Bilim University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, İstanbul, Turkey Objective:Green tea (Camellia sinensis) is one of the most consumed beverages in the world. C. sinensis consumption may prevent tumor and other diseases. Insulinomas are pancreatic islet cell tumors. In present study, the effects of the extract from C. sinensis were investigated in rat insulinoma INS-1 cells.Methods:C. sinensis leaves were collected in Rize, Turkey. The leaves were brewed in tap water for 20 and 40 minutes at 80°C. INS-1 cells were treated with different doses (1-5 mg/mL) of C. sinensis extract for 24 h. Cell viability, proliferation, death were examined. The malondialdehyde, glutathione and protein carbonyl levels were measured in INS-1 cells given C. sinensis extractResults:The cell viability and the percentage of proliferating cell nuclear antigen immunopositive cells decreased, while the percentage of TUNEL positive cells increased in INS-1 cells treated with the extract from C. sinensis according to control group. While malondialdehyde and protein carbonyl levels in INS-1 cells treated with C. sinensis extract decreased, glutathione levels increased according to control group.Conclusion:The results indicate that the extracts from C. sinensis inhibited proliferation and caused apoptosis in INS-1 cell. C. sinensis may be natural agent for supporting the treatment of pancreatic tumors. http://bezmialemscience.org/archives/archive-detail/article-preview/the-effects-of-icamellia-sinensis-i-extract-on-pro/20608 Camellia sinensis extractproliferationapoptosisoxidative stressINS-1 cells |
| spellingShingle | Ayşe Karatuğ KAÇAR Melike ERSÖZ Işıl SEZEKLER Zeynep Mine COŞKUN The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells Bezmiâlem Science Camellia sinensis extract proliferation apoptosis oxidative stress INS-1 cells |
| title | The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells |
| title_full | The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells |
| title_fullStr | The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells |
| title_full_unstemmed | The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells |
| title_short | The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells |
| title_sort | effects of camellia sinensis extract on proliferation apoptosis and oxidative stress in insulinoma ins 1 cells |
| topic | Camellia sinensis extract proliferation apoptosis oxidative stress INS-1 cells |
| url |
http://bezmialemscience.org/archives/archive-detail/article-preview/the-effects-of-icamellia-sinensis-i-extract-on-pro/20608
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