Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro
Objectives: We investigated remineralization effects of enamel binding peptide (EBP), WGNYAYK, on enamel subsurface demineralization in vitro.Methods: Bovine lower incisor crowns were used as subsurface enamel demineralization samples, and changes of EBP binding, remineraliztion rate, hardness and m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023103847 |
| _version_ | 1797337073277992960 |
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| author | Yoshihito Miyayoshi Hidenori Hamba Keiki Nakamura Hisako Ishizuka Takashi Muramatsu |
| author_facet | Yoshihito Miyayoshi Hidenori Hamba Keiki Nakamura Hisako Ishizuka Takashi Muramatsu |
| author_sort | Yoshihito Miyayoshi |
| collection | DOAJ |
| description | Objectives: We investigated remineralization effects of enamel binding peptide (EBP), WGNYAYK, on enamel subsurface demineralization in vitro.Methods: Bovine lower incisor crowns were used as subsurface enamel demineralization samples, and changes of EBP binding, remineraliztion rate, hardness and microstructure were investigated. Binding of EBP, remineralization rate, hardness and structural changes were investigated. Fluorescein isothiocyatate (FITC)-labeled EBPs (0.4 mM, 4.0 mM, and 7.0 mM) were applied to the samples for 30 min at 37 °C, with sample surfaces and cross-sections observed by confocal laser scanning microscope (CLSM). Mineralization analysis samples were divided into 4 experimental groups; distilled water (DW), EBP 0.4 mM, EBP 4.0 mM, and EBP 7.0 mM. Mineral density changes were measured by micro-CT with hardness measured by nano-indentation. Samples were also observed by scanning electron microscope (SEM) for surface and longitudinal microstructure. Results: CLSM images indicated that increased fluorescence was observed in the surface layer and up to about 20 μm below the surface layer. The remineralization rate was significantly higher for EBP 7.0 mM compared to DW (p = 0.008). Enamel surface hardness was significantly higher in all EBP groups compared to DW (p < 0.05) and was highest in the 7.0 mM group. SEM images showed obscuring of the superficial columnar structure in the 7.0 mM EBP group, indicating subsurface crystalline structure recovery. Conclusion: The results of this study suggest that EBP binds to demineralized enamel and promotes remineralization. |
| first_indexed | 2024-03-08T09:04:08Z |
| format | Article |
| id | doaj.art-1475384f4e9c437abead535bbcdc456a |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:04:08Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1475384f4e9c437abead535bbcdc456a2024-02-01T06:30:56ZengElsevierHeliyon2405-84402024-01-01101e23176Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitroYoshihito Miyayoshi0Hidenori Hamba1Keiki Nakamura2Hisako Ishizuka3Takashi Muramatsu4Department of Operative Dentistry, Cariology and Pulp Biology, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo 101-0061, JapanDepartment of Operative Dentistry, Cariology and Pulp Biology, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo 101-0061, JapanDepartment of Operative Dentistry, Cariology and Pulp Biology, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo 101-0061, JapanDepartment of Operative Dentistry, Cariology and Pulp Biology, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo 101-0061, JapanCorresponding author.; Department of Operative Dentistry, Cariology and Pulp Biology, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo 101-0061, JapanObjectives: We investigated remineralization effects of enamel binding peptide (EBP), WGNYAYK, on enamel subsurface demineralization in vitro.Methods: Bovine lower incisor crowns were used as subsurface enamel demineralization samples, and changes of EBP binding, remineraliztion rate, hardness and microstructure were investigated. Binding of EBP, remineralization rate, hardness and structural changes were investigated. Fluorescein isothiocyatate (FITC)-labeled EBPs (0.4 mM, 4.0 mM, and 7.0 mM) were applied to the samples for 30 min at 37 °C, with sample surfaces and cross-sections observed by confocal laser scanning microscope (CLSM). Mineralization analysis samples were divided into 4 experimental groups; distilled water (DW), EBP 0.4 mM, EBP 4.0 mM, and EBP 7.0 mM. Mineral density changes were measured by micro-CT with hardness measured by nano-indentation. Samples were also observed by scanning electron microscope (SEM) for surface and longitudinal microstructure. Results: CLSM images indicated that increased fluorescence was observed in the surface layer and up to about 20 μm below the surface layer. The remineralization rate was significantly higher for EBP 7.0 mM compared to DW (p = 0.008). Enamel surface hardness was significantly higher in all EBP groups compared to DW (p < 0.05) and was highest in the 7.0 mM group. SEM images showed obscuring of the superficial columnar structure in the 7.0 mM EBP group, indicating subsurface crystalline structure recovery. Conclusion: The results of this study suggest that EBP binds to demineralized enamel and promotes remineralization.http://www.sciencedirect.com/science/article/pii/S2405844023103847BiomineralizationEarly enamel cariesSubsurface lesionPeptide |
| spellingShingle | Yoshihito Miyayoshi Hidenori Hamba Keiki Nakamura Hisako Ishizuka Takashi Muramatsu Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro Heliyon Biomineralization Early enamel caries Subsurface lesion Peptide |
| title | Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro |
| title_full | Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro |
| title_fullStr | Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro |
| title_full_unstemmed | Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro |
| title_short | Remineralization effects of enamel binding peptide, WGNYAYK, on enamel subsurface demineralization in vitro |
| title_sort | remineralization effects of enamel binding peptide wgnyayk on enamel subsurface demineralization in vitro |
| topic | Biomineralization Early enamel caries Subsurface lesion Peptide |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023103847 |
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