Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine
Abstract Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcoh...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-05-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0407-0 |
| _version_ | 1818325480590278656 |
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| author | Lihua Zhang Qian Liu Hong Pan Xun Li Daoyi Guo |
| author_facet | Lihua Zhang Qian Liu Hong Pan Xun Li Daoyi Guo |
| author_sort | Lihua Zhang |
| collection | DOAJ |
| description | Abstract Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis. We establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine in E. coli with heterologous coexpression of aminotransferase (ARO8), keto acid decarboxylase (KDC) and aldehyde dehydrogenase H (AldH) gene. It was found that PAA transformation yield was up to ~94% from phenylalanine in E. coli and there was no by-product phenethyl alcohol was detected. Our results reveal the high efficiency of the RARE strain for production of PAA and indicate the potential industrial applicability of this microbial platform for PAA biosynthesis. |
| first_indexed | 2024-12-13T11:45:09Z |
| format | Article |
| id | doaj.art-147847ed3ffd4b61b9098cba57ef8a10 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T11:45:09Z |
| publishDate | 2017-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-147847ed3ffd4b61b9098cba57ef8a102022-12-21T23:47:31ZengSpringerOpenAMB Express2191-08552017-05-01711710.1186/s13568-017-0407-0Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanineLihua Zhang0Qian Liu1Hong Pan2Xun Li3Daoyi Guo4College of Life and Environmental Sciences, Gannan Normal UniversityCollege of Life and Environmental Sciences, Gannan Normal UniversityKey Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal UniversityKey Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal UniversityCollege of Life and Environmental Sciences, Gannan Normal UniversityAbstract Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis. We establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine in E. coli with heterologous coexpression of aminotransferase (ARO8), keto acid decarboxylase (KDC) and aldehyde dehydrogenase H (AldH) gene. It was found that PAA transformation yield was up to ~94% from phenylalanine in E. coli and there was no by-product phenethyl alcohol was detected. Our results reveal the high efficiency of the RARE strain for production of PAA and indicate the potential industrial applicability of this microbial platform for PAA biosynthesis.http://link.springer.com/article/10.1186/s13568-017-0407-0Metabolic engineeringPhenylacetic acidPhenylalaninePhenylacetaldehyde |
| spellingShingle | Lihua Zhang Qian Liu Hong Pan Xun Li Daoyi Guo Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine AMB Express Metabolic engineering Phenylacetic acid Phenylalanine Phenylacetaldehyde |
| title | Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine |
| title_full | Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine |
| title_fullStr | Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine |
| title_full_unstemmed | Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine |
| title_short | Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine |
| title_sort | metabolic engineering of escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine |
| topic | Metabolic engineering Phenylacetic acid Phenylalanine Phenylacetaldehyde |
| url | http://link.springer.com/article/10.1186/s13568-017-0407-0 |
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