Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users
Background: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that mus...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Dental Medicine, Universitas Airlangga
2018-03-01
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| Series: | Indonesian Journal of Dental Medicine |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/IJDM/article/view/14731 |
| _version_ | 1818648007379255296 |
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| author | Tiara Meilena Januar Cidie Fabiansyah Eha Djulaeha Hanoem Eka Hidayati |
| author_facet | Tiara Meilena Januar Cidie Fabiansyah Eha Djulaeha Hanoem Eka Hidayati |
| author_sort | Tiara Meilena |
| collection | DOAJ |
| description | Background: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that must be conducted is in vitro tests, one of which is MTT Assay. Samples used in this research were fibroblast cells (cultured BHK-21 cells) since fibroblasts are the most important and largest components in pulp, periodontal ligament, and gingiva. Purpose: This research aimed to determine toxicity effect of taro leaf extracts (Colocasia esculenta L. Schoot) on BHK-21 fibroblast cells. Methods: This research is an experimental laboratory research using Post Test Only Control Group design. The research treatment are the administration of Taro leaf extract (Colocasia esculenta L. Schoot) with 100%, 80%, 60%, and 40% concentration on BHK-21 fibroblast cell. Results: The fibroblast cell life percentage in 100%, 80%, 60%, and 40% concentration respectively are 106,3%, 50,9%, 36,2%, and 30,1%. The toxicity results are obtained using MTT assay technique after 24 hours. The optical density absorbency values are read by ELISA reader and represent life cell viability. Conclusion: Taro leaf extract (Colocasia esculenta L. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cells |
| first_indexed | 2024-12-17T01:11:35Z |
| format | Article |
| id | doaj.art-147a571f76b74920bc5b7e581ebe32e2 |
| institution | Directory Open Access Journal |
| issn | 2722-1253 |
| language | English |
| last_indexed | 2024-12-17T01:11:35Z |
| publishDate | 2018-03-01 |
| publisher | Faculty of Dental Medicine, Universitas Airlangga |
| record_format | Article |
| series | Indonesian Journal of Dental Medicine |
| spelling | doaj.art-147a571f76b74920bc5b7e581ebe32e22022-12-21T22:09:07ZengFaculty of Dental Medicine, Universitas AirlanggaIndonesian Journal of Dental Medicine2722-12532018-03-0111353910.20473/ijdm.v1i1.2018.35-397166Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture UsersTiara Meilena0Januar Cidie Fabiansyah1Eha Djulaeha2Hanoem Eka Hidayati3Faculty of Dental Medicine Universitas AirlanggaFaculty of Dental Medicine Universitas AirlanggaFaculty of Dental Medicine Universitas AirlanggaFaculty of Dental Medicine Universitas AirlanggaBackground: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that must be conducted is in vitro tests, one of which is MTT Assay. Samples used in this research were fibroblast cells (cultured BHK-21 cells) since fibroblasts are the most important and largest components in pulp, periodontal ligament, and gingiva. Purpose: This research aimed to determine toxicity effect of taro leaf extracts (Colocasia esculenta L. Schoot) on BHK-21 fibroblast cells. Methods: This research is an experimental laboratory research using Post Test Only Control Group design. The research treatment are the administration of Taro leaf extract (Colocasia esculenta L. Schoot) with 100%, 80%, 60%, and 40% concentration on BHK-21 fibroblast cell. Results: The fibroblast cell life percentage in 100%, 80%, 60%, and 40% concentration respectively are 106,3%, 50,9%, 36,2%, and 30,1%. The toxicity results are obtained using MTT assay technique after 24 hours. The optical density absorbency values are read by ELISA reader and represent life cell viability. Conclusion: Taro leaf extract (Colocasia esculenta L. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cellshttps://e-journal.unair.ac.id/IJDM/article/view/14731taro leaf extract, toxicity test, mtt assay, bhk-21 fibroblast cells |
| spellingShingle | Tiara Meilena Januar Cidie Fabiansyah Eha Djulaeha Hanoem Eka Hidayati Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users Indonesian Journal of Dental Medicine taro leaf extract, toxicity test, mtt assay, bhk-21 fibroblast cells |
| title | Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users |
| title_full | Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users |
| title_fullStr | Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users |
| title_full_unstemmed | Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users |
| title_short | Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users |
| title_sort | toxicity test on taro leaf extract colocasia esculenta l schoot as mouthwash to bhk 21 fibroblast cell culture in denture users |
| topic | taro leaf extract, toxicity test, mtt assay, bhk-21 fibroblast cells |
| url | https://e-journal.unair.ac.id/IJDM/article/view/14731 |
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