Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon.
Microbiota have been shown to have a great influence on functions of intestinal epithelial cells (ECs). The role of indole as a quorum-sensing (QS) molecule mediating intercellular signals in bacteria has been well appreciated. However, it remains unknown whether indole has beneficial effects on mai...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3835565?pdf=render |
| _version_ | 1818339839728156672 |
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| author | Yosuke Shimada Makoto Kinoshita Kazuo Harada Masafumi Mizutani Kazunori Masahata Hisako Kayama Kiyoshi Takeda |
| author_facet | Yosuke Shimada Makoto Kinoshita Kazuo Harada Masafumi Mizutani Kazunori Masahata Hisako Kayama Kiyoshi Takeda |
| author_sort | Yosuke Shimada |
| collection | DOAJ |
| description | Microbiota have been shown to have a great influence on functions of intestinal epithelial cells (ECs). The role of indole as a quorum-sensing (QS) molecule mediating intercellular signals in bacteria has been well appreciated. However, it remains unknown whether indole has beneficial effects on maintaining intestinal barriers in vivo. In this study, we analyzed the effect of indole on ECs using a germ free (GF) mouse model. GF mice showed decreased expression of junctional complex molecules in colonic ECs. The feces of specific pathogen-free (SPF) mice contained a high amount of indole; however the amount was significantly decreased in the feces of GF mice by 27-fold. Oral administration of indole-containing capsules resulted in increased expression of both tight junction (TJ)- and adherens junction (AJ)-associated molecules in colonic ECs in GF mice. In accordance with the increased expression of these junctional complex molecules, GF mice given indole-containing capsules showed higher resistance to dextran sodium sulfate (DSS)-induced colitis. A similar protective effect of indole on DSS-induced epithelial damage was also observed in mice bred in SPF conditions. These findings highlight the beneficial role of indole in establishing an epithelial barrier in vivo. |
| first_indexed | 2024-12-13T15:33:23Z |
| format | Article |
| id | doaj.art-147bda6dede244abb4956c28812339dd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T15:33:23Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-147bda6dede244abb4956c28812339dd2022-12-21T23:40:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e8060410.1371/journal.pone.0080604Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon.Yosuke ShimadaMakoto KinoshitaKazuo HaradaMasafumi MizutaniKazunori MasahataHisako KayamaKiyoshi TakedaMicrobiota have been shown to have a great influence on functions of intestinal epithelial cells (ECs). The role of indole as a quorum-sensing (QS) molecule mediating intercellular signals in bacteria has been well appreciated. However, it remains unknown whether indole has beneficial effects on maintaining intestinal barriers in vivo. In this study, we analyzed the effect of indole on ECs using a germ free (GF) mouse model. GF mice showed decreased expression of junctional complex molecules in colonic ECs. The feces of specific pathogen-free (SPF) mice contained a high amount of indole; however the amount was significantly decreased in the feces of GF mice by 27-fold. Oral administration of indole-containing capsules resulted in increased expression of both tight junction (TJ)- and adherens junction (AJ)-associated molecules in colonic ECs in GF mice. In accordance with the increased expression of these junctional complex molecules, GF mice given indole-containing capsules showed higher resistance to dextran sodium sulfate (DSS)-induced colitis. A similar protective effect of indole on DSS-induced epithelial damage was also observed in mice bred in SPF conditions. These findings highlight the beneficial role of indole in establishing an epithelial barrier in vivo.http://europepmc.org/articles/PMC3835565?pdf=render |
| spellingShingle | Yosuke Shimada Makoto Kinoshita Kazuo Harada Masafumi Mizutani Kazunori Masahata Hisako Kayama Kiyoshi Takeda Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon. PLoS ONE |
| title | Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon. |
| title_full | Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon. |
| title_fullStr | Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon. |
| title_full_unstemmed | Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon. |
| title_short | Commensal bacteria-dependent indole production enhances epithelial barrier function in the colon. |
| title_sort | commensal bacteria dependent indole production enhances epithelial barrier function in the colon |
| url | http://europepmc.org/articles/PMC3835565?pdf=render |
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