Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus
Abstract Background Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environmental and economic damage worldwide to date. Herein, a highly efficient,...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1530-8 |
| _version_ | 1811232830501421056 |
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| author | Ho Myeong Kim In Seong Choi Seoyoun Lee In Min Hwang Ho Hyun Chun Seung Gon Wi Jin-Cheol Kim Tae Young Shin Jong Cheol Kim Jae Su Kim Junheon Kim Hae Woong Park |
| author_facet | Ho Myeong Kim In Seong Choi Seoyoun Lee In Min Hwang Ho Hyun Chun Seung Gon Wi Jin-Cheol Kim Tae Young Shin Jong Cheol Kim Jae Su Kim Junheon Kim Hae Woong Park |
| author_sort | Ho Myeong Kim |
| collection | DOAJ |
| description | Abstract Background Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environmental and economic damage worldwide to date. Herein, a highly efficient, advanced process for producing destruxins (DTXs) from Miscanthus (MCT) is reported, along with an application strategy. Results The acetic acid–sodium chlorite pretreatment of MCT (AASC-MCT) is found to improve the monosaccharide production. Through biocatalytic conversion processes (simultaneous saccharification and cultivation), Metarhizium anisopliae JEF-279 can efficiently produce DTXs from 1% (w/v) AASC-MCT, i.e., DTX E (334.8 mg/L), A (288.8 mg/L), and B (48.6 mg/L). Monochamus alternatus (MA, Japanese pine sawyer) is known to act as a mediator transferring Bursaphelenchus xylophilus to pinewood. As B. xylophilus is associated with the occurrence of pine wilt disease, biological control of MA is a major strategy or controlling this disease. In this study, upon the application of a mixture of DTXs and protease-containing culture filtrate (PCF), complete mortality of MA is observed after a 5-day incubation. The MA immune system response is believed to cause an overexpression of actin and tropomyosin as a defense mechanism against the flaccid paralysis induced by the DTXs and PCF treatment. Conclusions These results suggest that MCT can be used as a major feedstock in the biorefinery industry and that DTXs can be applied as an insecticide for biological control of pine wilt disease via MA termination. |
| first_indexed | 2024-04-12T11:09:51Z |
| format | Article |
| id | doaj.art-147ced32f5cf4c1f9501daf0300caf01 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T11:09:51Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-147ced32f5cf4c1f9501daf0300caf012022-12-22T03:35:38ZengBMCBiotechnology for Biofuels1754-68342019-07-0112111010.1186/s13068-019-1530-8Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass MiscanthusHo Myeong Kim0In Seong Choi1Seoyoun Lee2In Min Hwang3Ho Hyun Chun4Seung Gon Wi5Jin-Cheol Kim6Tae Young Shin7Jong Cheol Kim8Jae Su Kim9Junheon Kim10Hae Woong Park11R&D Division, World Institute of KimchiR&D Division, World Institute of KimchiR&D Division, World Institute of KimchiR&D Division, World Institute of KimchiR&D Division, World Institute of KimchiAsian Pear Research Institute, Chonnam National UniversityDivision of Applied Bioscience & Biotechnology, Chonnam National UniversityDepartment of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National UniversityDepartment of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National UniversityDepartment of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National UniversityNational Institute of Forest ScienceR&D Division, World Institute of KimchiAbstract Background Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environmental and economic damage worldwide to date. Herein, a highly efficient, advanced process for producing destruxins (DTXs) from Miscanthus (MCT) is reported, along with an application strategy. Results The acetic acid–sodium chlorite pretreatment of MCT (AASC-MCT) is found to improve the monosaccharide production. Through biocatalytic conversion processes (simultaneous saccharification and cultivation), Metarhizium anisopliae JEF-279 can efficiently produce DTXs from 1% (w/v) AASC-MCT, i.e., DTX E (334.8 mg/L), A (288.8 mg/L), and B (48.6 mg/L). Monochamus alternatus (MA, Japanese pine sawyer) is known to act as a mediator transferring Bursaphelenchus xylophilus to pinewood. As B. xylophilus is associated with the occurrence of pine wilt disease, biological control of MA is a major strategy or controlling this disease. In this study, upon the application of a mixture of DTXs and protease-containing culture filtrate (PCF), complete mortality of MA is observed after a 5-day incubation. The MA immune system response is believed to cause an overexpression of actin and tropomyosin as a defense mechanism against the flaccid paralysis induced by the DTXs and PCF treatment. Conclusions These results suggest that MCT can be used as a major feedstock in the biorefinery industry and that DTXs can be applied as an insecticide for biological control of pine wilt disease via MA termination.http://link.springer.com/article/10.1186/s13068-019-1530-8BiorefineryDestruxinsPine wilt diseaseMiscanthusBiological control |
| spellingShingle | Ho Myeong Kim In Seong Choi Seoyoun Lee In Min Hwang Ho Hyun Chun Seung Gon Wi Jin-Cheol Kim Tae Young Shin Jong Cheol Kim Jae Su Kim Junheon Kim Hae Woong Park Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus Biotechnology for Biofuels Biorefinery Destruxins Pine wilt disease Miscanthus Biological control |
| title | Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus |
| title_full | Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus |
| title_fullStr | Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus |
| title_full_unstemmed | Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus |
| title_short | Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus |
| title_sort | advanced strategy to produce insecticidal destruxins from lignocellulosic biomass miscanthus |
| topic | Biorefinery Destruxins Pine wilt disease Miscanthus Biological control |
| url | http://link.springer.com/article/10.1186/s13068-019-1530-8 |
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