Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative
<i>Weizmannia coagulans</i> is an important potential probiotic with dual characteristics of <i>Bacillus</i> and <i>Lactobacillus</i>. This study describes a novel <i>Weizmannia coagulans</i> PL-W with excellent antibacterial activity isolated from Mon...
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/1/216 |
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| author | Yu Wang Zelin Gu Shiqi Zhang Pinglan Li |
| author_facet | Yu Wang Zelin Gu Shiqi Zhang Pinglan Li |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | <i>Weizmannia coagulans</i> is an important potential probiotic with dual characteristics of <i>Bacillus</i> and <i>Lactobacillus</i>. This study describes a novel <i>Weizmannia coagulans</i> PL-W with excellent antibacterial activity isolated from Mongolian traditional cheese, in which safety and probiotic potential were evaluated by complete genome sequencing. The crude bacteriocins of <i>W. coagulans</i> PL-W showed antibacterial activity against various foodborne pathogens, including <i>Listeria monocytogenes</i> CMCC 54,004, <i>Bacillus cereus</i> ATCC 14,579, and <i>Staphylococcus aureus</i> ATCC 25,923. Moreover, the crude bacteriocins have outstanding stability against pH, temperature, surfactants, and are sensitive to protease. The complete genome sequencing revealed <i>W. coagulans</i> PL-W consists of 3,666,052-base pair (bp) circular chromosomes with a GC content of 46.24% and 3485 protein-coding genes. It contains 84 tRNA, 10 23S rRNA, 10 16S rRNA, and 10 5S rRNA. In addition, no risk-related genes such as acquired antibiotic resistance genes, virulence, and pathogenic factors were identified, demonstrating that <i>W. coagulans</i> PL-W is safe to use. Furthermore, the presence of gene clusters involved in bacteriocin synthesis, adhesion-related genes, and genes contributing to acid and bile tolerance indicate that <i>W. coagulans</i> PL-W is a potential candidate probiotic. Thus, antimicrobial activity and genome characterization of <i>W. coagulans</i> PL-W demonstrate that it has extensive potential applications as a food protective culture. |
| first_indexed | 2024-03-11T10:00:31Z |
| format | Article |
| id | doaj.art-147e0d090bf04dc0b475b7da4a6133c0 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T10:00:31Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-147e0d090bf04dc0b475b7da4a6133c02023-11-16T15:23:59ZengMDPI AGFoods2304-81582023-01-0112121610.3390/foods12010216Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food PreservativeYu Wang0Zelin Gu1Shiqi Zhang2Pinglan Li3Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China<i>Weizmannia coagulans</i> is an important potential probiotic with dual characteristics of <i>Bacillus</i> and <i>Lactobacillus</i>. This study describes a novel <i>Weizmannia coagulans</i> PL-W with excellent antibacterial activity isolated from Mongolian traditional cheese, in which safety and probiotic potential were evaluated by complete genome sequencing. The crude bacteriocins of <i>W. coagulans</i> PL-W showed antibacterial activity against various foodborne pathogens, including <i>Listeria monocytogenes</i> CMCC 54,004, <i>Bacillus cereus</i> ATCC 14,579, and <i>Staphylococcus aureus</i> ATCC 25,923. Moreover, the crude bacteriocins have outstanding stability against pH, temperature, surfactants, and are sensitive to protease. The complete genome sequencing revealed <i>W. coagulans</i> PL-W consists of 3,666,052-base pair (bp) circular chromosomes with a GC content of 46.24% and 3485 protein-coding genes. It contains 84 tRNA, 10 23S rRNA, 10 16S rRNA, and 10 5S rRNA. In addition, no risk-related genes such as acquired antibiotic resistance genes, virulence, and pathogenic factors were identified, demonstrating that <i>W. coagulans</i> PL-W is safe to use. Furthermore, the presence of gene clusters involved in bacteriocin synthesis, adhesion-related genes, and genes contributing to acid and bile tolerance indicate that <i>W. coagulans</i> PL-W is a potential candidate probiotic. Thus, antimicrobial activity and genome characterization of <i>W. coagulans</i> PL-W demonstrate that it has extensive potential applications as a food protective culture.https://www.mdpi.com/2304-8158/12/1/216<i>Weizmannia coagulans</i>bacteriocincomplete genome sequencing |
| spellingShingle | Yu Wang Zelin Gu Shiqi Zhang Pinglan Li Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative Foods <i>Weizmannia coagulans</i> bacteriocin complete genome sequencing |
| title | Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative |
| title_full | Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative |
| title_fullStr | Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative |
| title_full_unstemmed | Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative |
| title_short | Complete Genome Sequencing Revealed the Potential Application of a Novel <i>Weizmannia coagulans</i> PL-W Production with Promising Bacteriocins in Food Preservative |
| title_sort | complete genome sequencing revealed the potential application of a novel i weizmannia coagulans i pl w production with promising bacteriocins in food preservative |
| topic | <i>Weizmannia coagulans</i> bacteriocin complete genome sequencing |
| url | https://www.mdpi.com/2304-8158/12/1/216 |
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