The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos
Epigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT) embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Her...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2016-05-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.115.026666 |
| _version_ | 1818569078613213184 |
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| author | Byungkuk Min Sunwha Cho Jung Sun Park Kyuheum Jeon Yong-Kook Kang |
| author_facet | Byungkuk Min Sunwha Cho Jung Sun Park Kyuheum Jeon Yong-Kook Kang |
| author_sort | Byungkuk Min |
| collection | DOAJ |
| description | Epigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT) embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. We observed that histone genes residing in the 1.5 Mb spanning the cow HIST1 cluster were coordinately downregulated in SCNT blastocysts. In contrast, both the nonhistone genes of this cluster, and histone genes elsewhere remained unaffected. This indicated that the downregulation was specific to HIST1 histone genes. We found that, after trichostatin A treatment, HIST1 histone genes were derepressed, and DNA methylation at their promoters was decreased to the level of in vitro fertilization embryos. Therefore, our results indicate that the reduced expression of HIST1 histone genes is a consequence of poor epigenetic reprogramming in SCNT blastocysts. |
| first_indexed | 2024-12-14T06:43:19Z |
| format | Article |
| id | doaj.art-1487d8520a3246f5b6e8f019bf87bdcf |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-14T06:43:19Z |
| publishDate | 2016-05-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-1487d8520a3246f5b6e8f019bf87bdcf2022-12-21T23:13:09ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362016-05-01651365137110.1534/g3.115.02666620The HIST1 Locus Escapes Reprogramming in Cloned Bovine EmbryosByungkuk MinSunwha ChoJung Sun ParkKyuheum JeonYong-Kook KangEpigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT) embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. We observed that histone genes residing in the 1.5 Mb spanning the cow HIST1 cluster were coordinately downregulated in SCNT blastocysts. In contrast, both the nonhistone genes of this cluster, and histone genes elsewhere remained unaffected. This indicated that the downregulation was specific to HIST1 histone genes. We found that, after trichostatin A treatment, HIST1 histone genes were derepressed, and DNA methylation at their promoters was decreased to the level of in vitro fertilization embryos. Therefore, our results indicate that the reduced expression of HIST1 histone genes is a consequence of poor epigenetic reprogramming in SCNT blastocysts.http://g3journal.org/lookup/doi/10.1534/g3.115.026666RNA-seqSCNTHIST1 clusterreprogrammingepigeneticsHDAC inhibitorDNA methylation |
| spellingShingle | Byungkuk Min Sunwha Cho Jung Sun Park Kyuheum Jeon Yong-Kook Kang The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos G3: Genes, Genomes, Genetics RNA-seq SCNT HIST1 cluster reprogramming epigenetics HDAC inhibitor DNA methylation |
| title | The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos |
| title_full | The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos |
| title_fullStr | The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos |
| title_full_unstemmed | The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos |
| title_short | The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos |
| title_sort | hist1 locus escapes reprogramming in cloned bovine embryos |
| topic | RNA-seq SCNT HIST1 cluster reprogramming epigenetics HDAC inhibitor DNA methylation |
| url | http://g3journal.org/lookup/doi/10.1534/g3.115.026666 |
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