Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila
Here, the authors demonstrate that the Legionella pneumophila T4SS effector protein Lpg2370 is a Ser/Thr kinase and a toxin of a tripartite HipBST toxin-antitoxin (TA) system. Structural data and biochemical analysis provide detailed insights into the toxin neutralization mechanism in the HipBST TA.
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32049-x |
| _version_ | 1828374376058191872 |
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| author | Xiangkai Zhen Yongyu Wu Jinli Ge Jiaqi Fu Le Ye Niannian Lin Zhijie Huang Zihe Liu Zhao-qing Luo Jiazhang Qiu Songying Ouyang |
| author_facet | Xiangkai Zhen Yongyu Wu Jinli Ge Jiaqi Fu Le Ye Niannian Lin Zhijie Huang Zihe Liu Zhao-qing Luo Jiazhang Qiu Songying Ouyang |
| author_sort | Xiangkai Zhen |
| collection | DOAJ |
| description | Here, the authors demonstrate that the Legionella pneumophila T4SS effector protein Lpg2370 is a Ser/Thr kinase and a toxin of a tripartite HipBST toxin-antitoxin (TA) system. Structural data and biochemical analysis provide detailed insights into the toxin neutralization mechanism in the HipBST TA. |
| first_indexed | 2024-04-14T07:31:40Z |
| format | Article |
| id | doaj.art-1487df0573c54c1a849daa2b17a70483 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-14T07:31:40Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1487df0573c54c1a849daa2b17a704832022-12-22T02:05:49ZengNature PortfolioNature Communications2041-17232022-07-0113111410.1038/s41467-022-32049-xMolecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophilaXiangkai Zhen0Yongyu Wu1Jinli Ge2Jiaqi Fu3Le Ye4Niannian Lin5Zhijie Huang6Zihe Liu7Zhao-qing Luo8Jiazhang Qiu9Songying Ouyang10Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityProvincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityState Key Laboratory for Zoonotic Diseases, College of Veterinary Medicine, Jilin UniversityPurdue Institute for Inflammation, Immunology and Infectious Disease and Department of Biological Sciences, Purdue UniversityProvincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityProvincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityProvincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityProvincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityPurdue Institute for Inflammation, Immunology and Infectious Disease and Department of Biological Sciences, Purdue UniversityState Key Laboratory for Zoonotic Diseases, College of Veterinary Medicine, Jilin UniversityProvincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal UniversityHere, the authors demonstrate that the Legionella pneumophila T4SS effector protein Lpg2370 is a Ser/Thr kinase and a toxin of a tripartite HipBST toxin-antitoxin (TA) system. Structural data and biochemical analysis provide detailed insights into the toxin neutralization mechanism in the HipBST TA.https://doi.org/10.1038/s41467-022-32049-x |
| spellingShingle | Xiangkai Zhen Yongyu Wu Jinli Ge Jiaqi Fu Le Ye Niannian Lin Zhijie Huang Zihe Liu Zhao-qing Luo Jiazhang Qiu Songying Ouyang Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila Nature Communications |
| title | Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila |
| title_full | Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila |
| title_fullStr | Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila |
| title_full_unstemmed | Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila |
| title_short | Molecular mechanism of toxin neutralization in the HipBST toxin-antitoxin system of Legionella pneumophila |
| title_sort | molecular mechanism of toxin neutralization in the hipbst toxin antitoxin system of legionella pneumophila |
| url | https://doi.org/10.1038/s41467-022-32049-x |
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