Microencapsulation and Fermentation of Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12

<p>Because of poor survival of probiotic bacteria, microencapsulation evolved from the immobilized cell culture technology used in the biotechnological industry. Two probiotic strains, <em>Bifidobacterium </em>(BB-12) and <em>Lactobacillus acidophilus </em>(LA-5) were i...

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Bibliographic Details
Main Authors: Maryam Yari, Jamshid Fooladi, Mohammad Ali Kargar Motlagh
Format: Article
Language:English
Published: Shahid Behehsti University of Medical Sciences 2015-09-01
Series:Applied Food Biotechnology
Subjects:
Online Access:http://journals.sbmu.ac.ir/afb/article/view/7711
Description
Summary:<p>Because of poor survival of probiotic bacteria, microencapsulation evolved from the immobilized cell culture technology used in the biotechnological industry. Two probiotic strains, <em>Bifidobacterium </em>(BB-12) and <em>Lactobacillus acidophilus </em>(LA-5) were immobilized in calcium alginate by extrusion method. Encapsulation parameters and efficacy of this method were evaluated. Growth factors of these two bacteria were also measured by culturing in 10-L fermenter. Growth curves were obtained with respect to optical density and dry biomass weight. Encapsulation yield was over than 60% in each experiment. Scanning electron microscopy (SEM) of Entrapment of cells in alginate matrix and cross-sections of dried bead were obtained and illustrated<em>. Bifidobacterium</em> have been shown better biotechnological properties.</p>
ISSN:2345-5357
2423-4214