Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis
Individual, naturally occurring Phlebotomus mongolensis and Phlebotomus caucasicus from Iran were screened for infections with the maternally inherited intracellular Rickettsia-like bacterium Wolbachia pipientis via targeting a major surface protein gene (wsp). The main objective of this study was t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2013-06-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400414&lng=en&tlng=en |
| _version_ | 1797715760596910080 |
|---|---|
| author | Parviz Parvizi Ali Bordbar Narmin Najafzadeh |
| author_facet | Parviz Parvizi Ali Bordbar Narmin Najafzadeh |
| author_sort | Parviz Parvizi |
| collection | DOAJ |
| description | Individual, naturally occurring Phlebotomus mongolensis and Phlebotomus caucasicus from Iran were screened for infections with the maternally inherited intracellular Rickettsia-like bacterium Wolbachia pipientis via targeting a major surface protein gene (wsp). The main objective of this study was to determine if W. pipientis could be detected in these species. The sandflies were screened using polymerase chain reaction to amplify a fragment of the Wolbachia surface protein gene. The obtained sequences were edited and aligned with database sequences to identify W. pipientis haplotypes. Two strains of Wolbachia were found. Strain Turk 54 (accession EU780683) is widespread and has previously been reported in Phlebotomus papatasi and other insects. Strain Turk 07 (accession KC576916) is a novel strain, found for first time in the two sister species. A-group strains of W. pipientis occur throughout much of the habitat of these sandflies. It is possible that Wolbachia is transferred via horizontal transmission. Horizontal transfer could shed light on sandfly control because Wolbachia is believed to drive a deleterious gene into sandflies that reduces their natural population density. With regard to our findings in this study, we can conclude that one species of sandfly can be infected with different Wolbachia strains and that different species of sandflies can be infected with a common strain. |
| first_indexed | 2024-03-12T08:11:31Z |
| format | Article |
| id | doaj.art-148931dabcd24d4a85b75ae8f332f00e |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:11:31Z |
| publishDate | 2013-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-148931dabcd24d4a85b75ae8f332f00e2023-09-02T19:06:42ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602013-06-01108441442010.1590/S0074-0276108042013004S0074-02762013000400414Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasisParviz ParviziAli BordbarNarmin NajafzadehIndividual, naturally occurring Phlebotomus mongolensis and Phlebotomus caucasicus from Iran were screened for infections with the maternally inherited intracellular Rickettsia-like bacterium Wolbachia pipientis via targeting a major surface protein gene (wsp). The main objective of this study was to determine if W. pipientis could be detected in these species. The sandflies were screened using polymerase chain reaction to amplify a fragment of the Wolbachia surface protein gene. The obtained sequences were edited and aligned with database sequences to identify W. pipientis haplotypes. Two strains of Wolbachia were found. Strain Turk 54 (accession EU780683) is widespread and has previously been reported in Phlebotomus papatasi and other insects. Strain Turk 07 (accession KC576916) is a novel strain, found for first time in the two sister species. A-group strains of W. pipientis occur throughout much of the habitat of these sandflies. It is possible that Wolbachia is transferred via horizontal transmission. Horizontal transfer could shed light on sandfly control because Wolbachia is believed to drive a deleterious gene into sandflies that reduces their natural population density. With regard to our findings in this study, we can conclude that one species of sandfly can be infected with different Wolbachia strains and that different species of sandflies can be infected with a common strain.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400414&lng=en&tlng=enWolbachia pipientiswsp geneParaphlebotomusL. majorIran |
| spellingShingle | Parviz Parvizi Ali Bordbar Narmin Najafzadeh Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis Memorias do Instituto Oswaldo Cruz Wolbachia pipientis wsp gene Paraphlebotomus L. major Iran |
| title | Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis |
| title_full | Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis |
| title_fullStr | Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis |
| title_full_unstemmed | Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis |
| title_short | Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis |
| title_sort | detection of wolbachia pipientis including a new strain containing the wsp gene in two sister species of paraphlebotomus sandflies potential vectors of zoonotic cutaneous leishmaniasis |
| topic | Wolbachia pipientis wsp gene Paraphlebotomus L. major Iran |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400414&lng=en&tlng=en |
| work_keys_str_mv | AT parvizparvizi detectionofwolbachiapipientisincludinganewstraincontainingthewspgeneintwosisterspeciesofparaphlebotomussandfliespotentialvectorsofzoonoticcutaneousleishmaniasis AT alibordbar detectionofwolbachiapipientisincludinganewstraincontainingthewspgeneintwosisterspeciesofparaphlebotomussandfliespotentialvectorsofzoonoticcutaneousleishmaniasis AT narminnajafzadeh detectionofwolbachiapipientisincludinganewstraincontainingthewspgeneintwosisterspeciesofparaphlebotomussandfliespotentialvectorsofzoonoticcutaneousleishmaniasis |