Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China
Japonica rice is widely planted in north-eastern China because of its superior food quality and stable grain yields. Nitrogen (N) is an essential element for rice growth, and development and its availability directly impacts on rice yields. The knowledge of N uptake and its utilization characteristi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01061/full |
| _version_ | 1819181299068305408 |
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| author | Jun Yi Jiping Gao Wenzhong Zhang Chen Zhao Yan Wang Xiaoxi Zhen |
| author_facet | Jun Yi Jiping Gao Wenzhong Zhang Chen Zhao Yan Wang Xiaoxi Zhen |
| author_sort | Jun Yi |
| collection | DOAJ |
| description | Japonica rice is widely planted in north-eastern China because of its superior food quality and stable grain yields. Nitrogen (N) is an essential element for rice growth, and development and its availability directly impacts on rice yields. The knowledge of N uptake and its utilization characteristics in japonica are thus important areas of research. Three japonica rice cultivars, SN265, SN1401, and SN9816, which are planted across large areas of north-eastern China, were used here to evaluate the uptake and utilization along the life cycle of both ammonium (NH4+) and nitrate (NO3−) in hydroponically grown plants. The plants were grown in one of three different solutions with varying NH4+:NO3− ratios: 1:0, 0:1, and 1:1 (The total N content was 40 mg L−1 for each treatment). At the tillering stage, when only NO3− was provided, lower rates of N uptake and enzyme activities of three rice plants resulted in reduced tiller numbers. During the reproductive stage, the NH4+ and (NH4+) uptake rates in SN1401 were consistently maintained at high levels, whereas the rates in SN265 and SN9816 were significantly lower, across all three treatments. At the booting stage, when only NO3− was provided, SN1401 plants had significantly higher expression levels of OsNRT2.1 and OsNRT2.2, higher activity of nitrate reductase in the roots, and higher activity levels of glutamine synthetase and glutamate synthase in the leaves, compared with the SN265 and SN9816 plants. The higher enzyme activity was beneficial to the secondary assimilation of N, which ultimately promoted panicle development in SN1401. Consequently, the grain yield per plant of SN1401 was the highest with solutions of both NH4+ and NO3−. These results indicate that selecting a rice cultivar with higher utilization of NO3− is beneficial for increasing the number of grains per panicle, grain yield, and N use efficiency. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T22:28:01Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-148a2fe379444044bc40812dae219f742022-12-21T18:10:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-09-011010.3389/fpls.2019.01061424504Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern ChinaJun YiJiping GaoWenzhong ZhangChen ZhaoYan WangXiaoxi ZhenJaponica rice is widely planted in north-eastern China because of its superior food quality and stable grain yields. Nitrogen (N) is an essential element for rice growth, and development and its availability directly impacts on rice yields. The knowledge of N uptake and its utilization characteristics in japonica are thus important areas of research. Three japonica rice cultivars, SN265, SN1401, and SN9816, which are planted across large areas of north-eastern China, were used here to evaluate the uptake and utilization along the life cycle of both ammonium (NH4+) and nitrate (NO3−) in hydroponically grown plants. The plants were grown in one of three different solutions with varying NH4+:NO3− ratios: 1:0, 0:1, and 1:1 (The total N content was 40 mg L−1 for each treatment). At the tillering stage, when only NO3− was provided, lower rates of N uptake and enzyme activities of three rice plants resulted in reduced tiller numbers. During the reproductive stage, the NH4+ and (NH4+) uptake rates in SN1401 were consistently maintained at high levels, whereas the rates in SN265 and SN9816 were significantly lower, across all three treatments. At the booting stage, when only NO3− was provided, SN1401 plants had significantly higher expression levels of OsNRT2.1 and OsNRT2.2, higher activity of nitrate reductase in the roots, and higher activity levels of glutamine synthetase and glutamate synthase in the leaves, compared with the SN265 and SN9816 plants. The higher enzyme activity was beneficial to the secondary assimilation of N, which ultimately promoted panicle development in SN1401. Consequently, the grain yield per plant of SN1401 was the highest with solutions of both NH4+ and NO3−. These results indicate that selecting a rice cultivar with higher utilization of NO3− is beneficial for increasing the number of grains per panicle, grain yield, and N use efficiency.https://www.frontiersin.org/article/10.3389/fpls.2019.01061/fulljaponica ricenitrogen metabolismnitrogen use efficiencygrain yieldpanicle |
| spellingShingle | Jun Yi Jiping Gao Wenzhong Zhang Chen Zhao Yan Wang Xiaoxi Zhen Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China Frontiers in Plant Science japonica rice nitrogen metabolism nitrogen use efficiency grain yield panicle |
| title | Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China |
| title_full | Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China |
| title_fullStr | Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China |
| title_full_unstemmed | Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China |
| title_short | Differential Uptake and Utilization of Two Forms of Nitrogen in Japonica Rice Cultivars From North-Eastern China |
| title_sort | differential uptake and utilization of two forms of nitrogen in japonica rice cultivars from north eastern china |
| topic | japonica rice nitrogen metabolism nitrogen use efficiency grain yield panicle |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01061/full |
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