Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids.
The consumption of soybean protein has well-known favorable metabolic effects (e.g., reduced body weight, body fat, hyperglycemia, insulin resistance, hepatic steatosis, and lipogenesis). These effects of soy protein have been linked to modulation by the gut microbiota; however, the dynamic interpla...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6089412?pdf=render |
| _version_ | 1811319751910096896 |
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| author | Keita Watanabe Miki Igarashi Xuan Li Akiho Nakatani Junki Miyamoto Yuka Inaba Asuka Sutou Tsutomu Saito Takumi Sato Nobuhiko Tachibana Hiroshi Inoue Ikuo Kimura |
| author_facet | Keita Watanabe Miki Igarashi Xuan Li Akiho Nakatani Junki Miyamoto Yuka Inaba Asuka Sutou Tsutomu Saito Takumi Sato Nobuhiko Tachibana Hiroshi Inoue Ikuo Kimura |
| author_sort | Keita Watanabe |
| collection | DOAJ |
| description | The consumption of soybean protein has well-known favorable metabolic effects (e.g., reduced body weight, body fat, hyperglycemia, insulin resistance, hepatic steatosis, and lipogenesis). These effects of soy protein have been linked to modulation by the gut microbiota; however, the dynamic interplay among these factors remains unclear. Accordingly, we examined the metabolic phenotype, intestinal BA pool, and the gut microbiome of male C57BL/6 mice that were randomized to receive either a regular high-fat diet (HFD) or HFD that contained soybean protein isolate (SPI) in place of dairy protein. The intake of SPI significantly reduced the HFD-induced weight gain and adipose tissue mass accumulation and attenuated hepatic steatosis. Along with an enhancement in the secretion of intestinal Glucagon-like peptide-1 (GLP-1), an enlarged cecal BA pool with an elevated secondary/primary BA ratio was observed in the mice that consumed SPI, while fecal BA excretion remained unaltered. SPI also elicited dramatic changes in the gut microbiome, characterized by an expansion of taxa that may be involved in the biotransformation of BAs. The observed effects were abolished in germ-free (GF) mice, indicating that they were dependent on the microbiota. These findings collectively indicate that the metabolic benefits of SPI under the HFD regime may arise from a microbiota-driven increase in BA transformation and increase in GLP-1 secretion. |
| first_indexed | 2024-04-13T12:48:05Z |
| format | Article |
| id | doaj.art-148ac628034d40c8ab51abfd60cc1b74 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T12:48:05Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-148ac628034d40c8ab51abfd60cc1b742022-12-22T02:46:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020208310.1371/journal.pone.0202083Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids.Keita WatanabeMiki IgarashiXuan LiAkiho NakataniJunki MiyamotoYuka InabaAsuka SutouTsutomu SaitoTakumi SatoNobuhiko TachibanaHiroshi InoueIkuo KimuraThe consumption of soybean protein has well-known favorable metabolic effects (e.g., reduced body weight, body fat, hyperglycemia, insulin resistance, hepatic steatosis, and lipogenesis). These effects of soy protein have been linked to modulation by the gut microbiota; however, the dynamic interplay among these factors remains unclear. Accordingly, we examined the metabolic phenotype, intestinal BA pool, and the gut microbiome of male C57BL/6 mice that were randomized to receive either a regular high-fat diet (HFD) or HFD that contained soybean protein isolate (SPI) in place of dairy protein. The intake of SPI significantly reduced the HFD-induced weight gain and adipose tissue mass accumulation and attenuated hepatic steatosis. Along with an enhancement in the secretion of intestinal Glucagon-like peptide-1 (GLP-1), an enlarged cecal BA pool with an elevated secondary/primary BA ratio was observed in the mice that consumed SPI, while fecal BA excretion remained unaltered. SPI also elicited dramatic changes in the gut microbiome, characterized by an expansion of taxa that may be involved in the biotransformation of BAs. The observed effects were abolished in germ-free (GF) mice, indicating that they were dependent on the microbiota. These findings collectively indicate that the metabolic benefits of SPI under the HFD regime may arise from a microbiota-driven increase in BA transformation and increase in GLP-1 secretion.http://europepmc.org/articles/PMC6089412?pdf=render |
| spellingShingle | Keita Watanabe Miki Igarashi Xuan Li Akiho Nakatani Junki Miyamoto Yuka Inaba Asuka Sutou Tsutomu Saito Takumi Sato Nobuhiko Tachibana Hiroshi Inoue Ikuo Kimura Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids. PLoS ONE |
| title | Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids. |
| title_full | Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids. |
| title_fullStr | Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids. |
| title_full_unstemmed | Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids. |
| title_short | Dietary soybean protein ameliorates high-fat diet-induced obesity by modifying the gut microbiota-dependent biotransformation of bile acids. |
| title_sort | dietary soybean protein ameliorates high fat diet induced obesity by modifying the gut microbiota dependent biotransformation of bile acids |
| url | http://europepmc.org/articles/PMC6089412?pdf=render |
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