A sequence-based 163plex microhaplotype assay for forensic DNA analysis
Novel genetic marker microhaplotype has led to an upsurge in forensic genetic research. This study established a 163 microhaplotype (MH) multiplex assay based on next-generation sequencing (NGS) and evaluated the assay’s performance and applicability. Our results showed that the 163 MH assay was acc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.988223/full |
| _version_ | 1797997322039197696 |
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| author | Ruiyang Tao Qi Yang Ruocheng Xia Xiaochun Zhang Anqi Chen Chengtao Li Suhua Zhang |
| author_facet | Ruiyang Tao Qi Yang Ruocheng Xia Xiaochun Zhang Anqi Chen Chengtao Li Suhua Zhang |
| author_sort | Ruiyang Tao |
| collection | DOAJ |
| description | Novel genetic marker microhaplotype has led to an upsurge in forensic genetic research. This study established a 163 microhaplotype (MH) multiplex assay based on next-generation sequencing (NGS) and evaluated the assay’s performance and applicability. Our results showed that the 163 MH assay was accurate, repeatable and reliable, and could distinguish between African, European-American, Southern Asia and Eastern Asia populations. Among the 163 MH makers, 48 MHs with Ae > 3.0 in China Eastern Han were selected and confirmed to be highly polymorphic, with a combined power of discrimination of 1–8.26 × 10–44 and the combined power of exclusion in duos and trios of 1–1.26 × 10–8 and 1–8.27 × 10–16, respectively. Moreover, the mixture study demonstrated the realizability of the MHs in deconvoluting mixtures with different proportions of two to five-person. In conclusion, our findings support the use of this MH assay for ancestry inference, human identification, paternity testing and mixture deconvolution in forensic research. |
| first_indexed | 2024-04-11T10:31:05Z |
| format | Article |
| id | doaj.art-14931a5f56f54f5bbadef860f72da8fb |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T10:31:05Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj.art-14931a5f56f54f5bbadef860f72da8fb2022-12-22T04:29:25ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-10-011310.3389/fgene.2022.988223988223A sequence-based 163plex microhaplotype assay for forensic DNA analysisRuiyang TaoQi YangRuocheng XiaXiaochun ZhangAnqi ChenChengtao LiSuhua ZhangNovel genetic marker microhaplotype has led to an upsurge in forensic genetic research. This study established a 163 microhaplotype (MH) multiplex assay based on next-generation sequencing (NGS) and evaluated the assay’s performance and applicability. Our results showed that the 163 MH assay was accurate, repeatable and reliable, and could distinguish between African, European-American, Southern Asia and Eastern Asia populations. Among the 163 MH makers, 48 MHs with Ae > 3.0 in China Eastern Han were selected and confirmed to be highly polymorphic, with a combined power of discrimination of 1–8.26 × 10–44 and the combined power of exclusion in duos and trios of 1–1.26 × 10–8 and 1–8.27 × 10–16, respectively. Moreover, the mixture study demonstrated the realizability of the MHs in deconvoluting mixtures with different proportions of two to five-person. In conclusion, our findings support the use of this MH assay for ancestry inference, human identification, paternity testing and mixture deconvolution in forensic research.https://www.frontiersin.org/articles/10.3389/fgene.2022.988223/fullforensic geneticsmicrohaplotype (MH)next-generation sequencing (NGS)mixture deconvolutionancestry inference |
| spellingShingle | Ruiyang Tao Qi Yang Ruocheng Xia Xiaochun Zhang Anqi Chen Chengtao Li Suhua Zhang A sequence-based 163plex microhaplotype assay for forensic DNA analysis Frontiers in Genetics forensic genetics microhaplotype (MH) next-generation sequencing (NGS) mixture deconvolution ancestry inference |
| title | A sequence-based 163plex microhaplotype assay for forensic DNA analysis |
| title_full | A sequence-based 163plex microhaplotype assay for forensic DNA analysis |
| title_fullStr | A sequence-based 163plex microhaplotype assay for forensic DNA analysis |
| title_full_unstemmed | A sequence-based 163plex microhaplotype assay for forensic DNA analysis |
| title_short | A sequence-based 163plex microhaplotype assay for forensic DNA analysis |
| title_sort | sequence based 163plex microhaplotype assay for forensic dna analysis |
| topic | forensic genetics microhaplotype (MH) next-generation sequencing (NGS) mixture deconvolution ancestry inference |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.988223/full |
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