Identification and characterization of novel abdominal and pelvic brown adipose depots in mice
Brown adipose tissue (BAT) generates heat through non-shivering thermogenesis, and increasing BAT amounts or activity could facilitate obesity treatment and provide metabolic benefits. In mice, BAT has been reported in perirenal, thoracic and cranial sites. Here, we describe new pelvic and lower abd...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Adipocyte |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2022.2133415 |
| _version_ | 1811340335734849536 |
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| author | Ana M. Mesa Theresa I. Medrano Vijay K. Sirohi William H. Walker Richard D. Johnson Sergei G. Tevosian Angie M. Adkin Paul S. Cooke |
| author_facet | Ana M. Mesa Theresa I. Medrano Vijay K. Sirohi William H. Walker Richard D. Johnson Sergei G. Tevosian Angie M. Adkin Paul S. Cooke |
| author_sort | Ana M. Mesa |
| collection | DOAJ |
| description | Brown adipose tissue (BAT) generates heat through non-shivering thermogenesis, and increasing BAT amounts or activity could facilitate obesity treatment and provide metabolic benefits. In mice, BAT has been reported in perirenal, thoracic and cranial sites. Here, we describe new pelvic and lower abdominal BAT depots located around the urethra, internal reproductive and urinary tract organs and major lower pelvic blood vessels, as well as between adjacent muscles where the upper hind leg meets the abdominal cavity. Immunohistochemical, western blot and PCR analyses revealed that these tissues expressed BAT markers such as uncoupling protein 1 (UCP1) and CIDEA, but not white adipose markers, and β3-adrenergic stimulation increased UCP1 amounts, a classic characteristic of BAT tissue. The newly identified BAT stores contained extensive sympathetic innervation with high mitochondrial density and multilocular lipid droplets similar to interscapular BAT. BAT repositories were present and functional neonatally, and showed developmental changes between the neonatal and adult periods. In summary, several new depots showing classical BAT characteristics are reported and characterized in the lower abdominal/pelvic region of mice. These BAT stores are likely significant metabolic regulators in the mouse and some data suggests that similar BAT depots may also exist in humans. |
| first_indexed | 2024-04-13T18:40:25Z |
| format | Article |
| id | doaj.art-1494595abbc045c4983e205567fa95d4 |
| institution | Directory Open Access Journal |
| issn | 2162-3945 2162-397X |
| language | English |
| last_indexed | 2024-04-13T18:40:25Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj.art-1494595abbc045c4983e205567fa95d42022-12-22T02:34:44ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2022-12-0111161662910.1080/21623945.2022.2133415Identification and characterization of novel abdominal and pelvic brown adipose depots in miceAna M. Mesa0Theresa I. Medrano1Vijay K. Sirohi2William H. Walker3Richard D. Johnson4Sergei G. Tevosian5Angie M. Adkin6Paul S. Cooke7Department of Physiological Sciences, University of Florida, Gainesville, FL, USADepartment of Physiological Sciences, University of Florida, Gainesville, FL, USADepartment of Physiological Sciences, University of Florida, Gainesville, FL, USADepartment of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh and Magee-Womens Research Institute, Pittsburgh, PA, USADepartment of Physiological Sciences, University of Florida, Gainesville, FL, USADepartment of Physiological Sciences, University of Florida, Gainesville, FL, USADepartment of Physiological Sciences, University of Florida, Gainesville, FL, USADepartment of Physiological Sciences, University of Florida, Gainesville, FL, USABrown adipose tissue (BAT) generates heat through non-shivering thermogenesis, and increasing BAT amounts or activity could facilitate obesity treatment and provide metabolic benefits. In mice, BAT has been reported in perirenal, thoracic and cranial sites. Here, we describe new pelvic and lower abdominal BAT depots located around the urethra, internal reproductive and urinary tract organs and major lower pelvic blood vessels, as well as between adjacent muscles where the upper hind leg meets the abdominal cavity. Immunohistochemical, western blot and PCR analyses revealed that these tissues expressed BAT markers such as uncoupling protein 1 (UCP1) and CIDEA, but not white adipose markers, and β3-adrenergic stimulation increased UCP1 amounts, a classic characteristic of BAT tissue. The newly identified BAT stores contained extensive sympathetic innervation with high mitochondrial density and multilocular lipid droplets similar to interscapular BAT. BAT repositories were present and functional neonatally, and showed developmental changes between the neonatal and adult periods. In summary, several new depots showing classical BAT characteristics are reported and characterized in the lower abdominal/pelvic region of mice. These BAT stores are likely significant metabolic regulators in the mouse and some data suggests that similar BAT depots may also exist in humans.https://www.tandfonline.com/doi/10.1080/21623945.2022.2133415UCP1thermogenesisureteruterusmuscle |
| spellingShingle | Ana M. Mesa Theresa I. Medrano Vijay K. Sirohi William H. Walker Richard D. Johnson Sergei G. Tevosian Angie M. Adkin Paul S. Cooke Identification and characterization of novel abdominal and pelvic brown adipose depots in mice Adipocyte UCP1 thermogenesis ureter uterus muscle |
| title | Identification and characterization of novel abdominal and pelvic brown adipose depots in mice |
| title_full | Identification and characterization of novel abdominal and pelvic brown adipose depots in mice |
| title_fullStr | Identification and characterization of novel abdominal and pelvic brown adipose depots in mice |
| title_full_unstemmed | Identification and characterization of novel abdominal and pelvic brown adipose depots in mice |
| title_short | Identification and characterization of novel abdominal and pelvic brown adipose depots in mice |
| title_sort | identification and characterization of novel abdominal and pelvic brown adipose depots in mice |
| topic | UCP1 thermogenesis ureter uterus muscle |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2022.2133415 |
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