Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles
Optimizing the biological identity of nanoparticles (NPs) for efficient tumor uptake remains challenging. The controlled formation of a protein corona on NPs through protein absorption from biofluids could favor a biological identity that enables tumor accumulation. To increase the diversity of prot...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Biomimetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-7673/7/4/219 |
| _version_ | 1827641585670553600 |
|---|---|
| author | Daniel Nierenberg Orielyz Flores David Fox Yuen Yee Li Sip Caroline M. Finn Heba Ghozlan Amanda Cox Melanie Coathup Karl Kai McKinstry Lei Zhai Annette R. Khaled |
| author_facet | Daniel Nierenberg Orielyz Flores David Fox Yuen Yee Li Sip Caroline M. Finn Heba Ghozlan Amanda Cox Melanie Coathup Karl Kai McKinstry Lei Zhai Annette R. Khaled |
| author_sort | Daniel Nierenberg |
| collection | DOAJ |
| description | Optimizing the biological identity of nanoparticles (NPs) for efficient tumor uptake remains challenging. The controlled formation of a protein corona on NPs through protein absorption from biofluids could favor a biological identity that enables tumor accumulation. To increase the diversity of proteins absorbed by NPs, sera derived from Influenza A virus (IAV)-infected mice were used to pre-coat NPs formed using a hyperbranched polyester polymer (HBPE-NPs). HBPE-NPs, encapsulating a tracking dye or cancer drug, were treated with sera from days 3–6 of IAV infection (VS3-6), and uptake of HBPE-NPs by breast cancer cells was examined. Cancer cells demonstrated better uptake of HBPE-NPs pre-treated with VS3-6 over polyethylene glycol (PEG)-HBPE-NPs, a standard NP surface modification. The uptake of VS5 pre-treated HBPE-NPs by monocytic cells (THP-1) was decreased over PEG-HBPE-NPs. VS5-treated HBPE-NPs delivered a cancer drug more efficiently and displayed better <i>in vivo</i> distribution over controls, remaining stable even after interacting with endothelial cells. Using a proteomics approach, proteins absorbed from sera-treated HBPE-NPs were identified, such as thrombospondin-1 (TSP-1), that could bind multiple cancer cell receptors. Our findings indicate that serum collected during an immune response to infection is a rich source of macromolecules that are absorbed by NPs and modulate their biological identity, achieving rationally designed uptake by targeted cell types. |
| first_indexed | 2024-03-09T17:16:55Z |
| format | Article |
| id | doaj.art-14948fc2c5f841329c43e0ba2b9cbce6 |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-09T17:16:55Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-14948fc2c5f841329c43e0ba2b9cbce62023-11-24T13:31:40ZengMDPI AGBiomimetics2313-76732022-11-017421910.3390/biomimetics7040219Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric NanoparticlesDaniel Nierenberg0Orielyz Flores1David Fox2Yuen Yee Li Sip3Caroline M. Finn4Heba Ghozlan5Amanda Cox6Melanie Coathup7Karl Kai McKinstry8Lei Zhai9Annette R. Khaled10Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USANanoScience Technology Science Center, University of Central Florida, Orlando, FL 32826, USANanoScience Technology Science Center, University of Central Florida, Orlando, FL 32826, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USABiionix Cluster and Department of Internal Medicine, College of Medicine, University of Central Florida, Orlando, FL 32827, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USANanoScience Technology Science Center, University of Central Florida, Orlando, FL 32826, USABurnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32827, USAOptimizing the biological identity of nanoparticles (NPs) for efficient tumor uptake remains challenging. The controlled formation of a protein corona on NPs through protein absorption from biofluids could favor a biological identity that enables tumor accumulation. To increase the diversity of proteins absorbed by NPs, sera derived from Influenza A virus (IAV)-infected mice were used to pre-coat NPs formed using a hyperbranched polyester polymer (HBPE-NPs). HBPE-NPs, encapsulating a tracking dye or cancer drug, were treated with sera from days 3–6 of IAV infection (VS3-6), and uptake of HBPE-NPs by breast cancer cells was examined. Cancer cells demonstrated better uptake of HBPE-NPs pre-treated with VS3-6 over polyethylene glycol (PEG)-HBPE-NPs, a standard NP surface modification. The uptake of VS5 pre-treated HBPE-NPs by monocytic cells (THP-1) was decreased over PEG-HBPE-NPs. VS5-treated HBPE-NPs delivered a cancer drug more efficiently and displayed better <i>in vivo</i> distribution over controls, remaining stable even after interacting with endothelial cells. Using a proteomics approach, proteins absorbed from sera-treated HBPE-NPs were identified, such as thrombospondin-1 (TSP-1), that could bind multiple cancer cell receptors. Our findings indicate that serum collected during an immune response to infection is a rich source of macromolecules that are absorbed by NPs and modulate their biological identity, achieving rationally designed uptake by targeted cell types.https://www.mdpi.com/2313-7673/7/4/219drug deliverytumorpaclitaxelprotein coronananomedicine |
| spellingShingle | Daniel Nierenberg Orielyz Flores David Fox Yuen Yee Li Sip Caroline M. Finn Heba Ghozlan Amanda Cox Melanie Coathup Karl Kai McKinstry Lei Zhai Annette R. Khaled Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles Biomimetics drug delivery tumor paclitaxel protein corona nanomedicine |
| title | Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles |
| title_full | Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles |
| title_fullStr | Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles |
| title_full_unstemmed | Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles |
| title_short | Macromolecules Absorbed from Influenza Infection-Based Sera Modulate the Cellular Uptake of Polymeric Nanoparticles |
| title_sort | macromolecules absorbed from influenza infection based sera modulate the cellular uptake of polymeric nanoparticles |
| topic | drug delivery tumor paclitaxel protein corona nanomedicine |
| url | https://www.mdpi.com/2313-7673/7/4/219 |
| work_keys_str_mv | AT danielnierenberg macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT orielyzflores macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT davidfox macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT yuenyeelisip macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT carolinemfinn macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT hebaghozlan macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT amandacox macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT melaniecoathup macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT karlkaimckinstry macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT leizhai macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles AT annetterkhaled macromoleculesabsorbedfrominfluenzainfectionbasedseramodulatethecellularuptakeofpolymericnanoparticles |