Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis
Abstract Background Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. Results Herein, investigations revealed that chilli...
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03507-x |
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| author | Shanshan Zhu Denghui Gu Caiping Lu Caixia Zhang Juanjuan Chen Rui Yang Qijun Luo Tiegan Wang Peng Zhang Haimin Chen |
| author_facet | Shanshan Zhu Denghui Gu Caiping Lu Caixia Zhang Juanjuan Chen Rui Yang Qijun Luo Tiegan Wang Peng Zhang Haimin Chen |
| author_sort | Shanshan Zhu |
| collection | DOAJ |
| description | Abstract Background Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. Results Herein, investigations revealed that chilling and freezing induced alterations in the physiological properties, gene transcriptional profiles and metabolite levels in the economically important red algae species, Neoporphyra haitanensis. Control samples (kept at 20 °C) were compared to chilled thalli (10 and 4 °C) and to thalli under − 4 °C conditions. Chilling stress did not affect the health or photosynthetic efficiency of gametophytes, but freezing conditions resulted in the arrest of growth, death of some cells and a decrease in photosynthetic activity as calculated by Fv/Fm. Transcriptome sequencing analysis revealed that the photosynthetic system was down-regulated along with genes associated with carbon fixation and primary metabolic biosynthesis. Adaptive mechanisms included an increase in unsaturated fatty acids levels to improve membrane fluidity, an increase in floridoside and isofloridoside content to enhance osmotic resistance, and an elevation in levels of some resistance-associated phytohormones (abscisic acid, salicylic acid, and methyl jasmonic acid). These physiochemical alterations occurred together with the upregulation of ribosome biogenesis. Conclusions N. haitanensis adopts multiple protective mechanisms to maintain homeostasis of cellular physiology in tolerance to cold stress. |
| first_indexed | 2024-12-17T08:17:29Z |
| format | Article |
| id | doaj.art-1495c314de6740e6bf9394c47d97034f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-17T08:17:29Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-1495c314de6740e6bf9394c47d97034f2022-12-21T21:57:01ZengBMCBMC Plant Biology1471-22292022-03-0122111710.1186/s12870-022-03507-xCold stress tolerance of the intertidal red alga Neoporphyra haitanensisShanshan Zhu0Denghui Gu1Caiping Lu2Caixia Zhang3Juanjuan Chen4Rui Yang5Qijun Luo6Tiegan Wang7Peng Zhang8Haimin Chen9State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityZhejiang Mariculture Research InstituteZhejiang Mariculture Research InstituteState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo UniversityAbstract Background Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. Results Herein, investigations revealed that chilling and freezing induced alterations in the physiological properties, gene transcriptional profiles and metabolite levels in the economically important red algae species, Neoporphyra haitanensis. Control samples (kept at 20 °C) were compared to chilled thalli (10 and 4 °C) and to thalli under − 4 °C conditions. Chilling stress did not affect the health or photosynthetic efficiency of gametophytes, but freezing conditions resulted in the arrest of growth, death of some cells and a decrease in photosynthetic activity as calculated by Fv/Fm. Transcriptome sequencing analysis revealed that the photosynthetic system was down-regulated along with genes associated with carbon fixation and primary metabolic biosynthesis. Adaptive mechanisms included an increase in unsaturated fatty acids levels to improve membrane fluidity, an increase in floridoside and isofloridoside content to enhance osmotic resistance, and an elevation in levels of some resistance-associated phytohormones (abscisic acid, salicylic acid, and methyl jasmonic acid). These physiochemical alterations occurred together with the upregulation of ribosome biogenesis. Conclusions N. haitanensis adopts multiple protective mechanisms to maintain homeostasis of cellular physiology in tolerance to cold stress.https://doi.org/10.1186/s12870-022-03507-xNeoporphyra haitanensisCold stressTranscriptome |
| spellingShingle | Shanshan Zhu Denghui Gu Caiping Lu Caixia Zhang Juanjuan Chen Rui Yang Qijun Luo Tiegan Wang Peng Zhang Haimin Chen Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis BMC Plant Biology Neoporphyra haitanensis Cold stress Transcriptome |
| title | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
| title_full | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
| title_fullStr | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
| title_full_unstemmed | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
| title_short | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
| title_sort | cold stress tolerance of the intertidal red alga neoporphyra haitanensis |
| topic | Neoporphyra haitanensis Cold stress Transcriptome |
| url | https://doi.org/10.1186/s12870-022-03507-x |
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