Blastema cells derived from rabbit ear show stem cell characteristics
Abstract Regeneration is a biological phenomenon, which takes place via two main mechanisms: first, dedifferentiation of mature cells followed by their differentiation into functional new cells and second, activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2011-06-01
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| Series: | Journal of Cell and Molecular Research |
| Subjects: | |
| Online Access: | https://jcmr.um.ac.ir/article_26173_50e2df6e04b7078055ab9e9bb43b2aa0.pdf |
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| author | Maryam Moghaddam Matin Morvarid Saeinasab Saeideh Nakhaei-Rad Mahdi Mirahmadi Nasser Mahdavi Shahri Mahmoud Mahmoudi Ahmad Reza Bahrami |
| author_facet | Maryam Moghaddam Matin Morvarid Saeinasab Saeideh Nakhaei-Rad Mahdi Mirahmadi Nasser Mahdavi Shahri Mahmoud Mahmoudi Ahmad Reza Bahrami |
| author_sort | Maryam Moghaddam Matin |
| collection | DOAJ |
| description | Abstract
Regeneration is a biological phenomenon, which takes place via two main mechanisms: first,
dedifferentiation of mature cells followed by their differentiation into functional new cells and second,
activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One of the best
examples of healing process in mammals is the regeneration of damaged pinna in rabbits by blastema tissue.
The aim of present study was to investigate culture requirements, proliferative properties and expression of
some stemness factors in cells derived from regenerating blastema tissue obtained from rabbit pinna in vitro.
The regenerating tissues were obtained from male New Zealand white rabbits by double punching of the pinna
and cell culture conditions were set to derive and enrich the self renewing cells for further characterisation. The
cells were subjected to survival and growth examinations in vitro, and expression of several stemness factors
was studied in these cells using reverse transcription polymerase chain reaction (RT-PCR). Results revealed
that the derived cells are rather immortal, as they have been growing for more than 120 passages in culture up
until this report. Furthermore, RT-PCR and flow cytometry analyses showed that these cells express a number
of stemness related genes including Oct4 and Sox2. In conclusion, in this study, stem like cells were derived
from blastema tissue of rabbit ears for the first time, showing great self renewing capacity, which provides a
suitable in vitro model for regeneration studies. Moreover, they could be considered as a good source of stem
like cells for future applications. |
| first_indexed | 2024-12-20T16:06:41Z |
| format | Article |
| id | doaj.art-1496fd720de54103934307ce181beafb |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-12-20T16:06:41Z |
| publishDate | 2011-06-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-1496fd720de54103934307ce181beafb2022-12-21T19:34:08ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642011-06-0131253010.22067/jcmr.v3i1.1311426173Blastema cells derived from rabbit ear show stem cell characteristicsMaryam Moghaddam MatinMorvarid SaeinasabSaeideh Nakhaei-RadMahdi MirahmadiNasser Mahdavi ShahriMahmoud MahmoudiAhmad Reza BahramiAbstract Regeneration is a biological phenomenon, which takes place via two main mechanisms: first, dedifferentiation of mature cells followed by their differentiation into functional new cells and second, activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. One of the best examples of healing process in mammals is the regeneration of damaged pinna in rabbits by blastema tissue. The aim of present study was to investigate culture requirements, proliferative properties and expression of some stemness factors in cells derived from regenerating blastema tissue obtained from rabbit pinna in vitro. The regenerating tissues were obtained from male New Zealand white rabbits by double punching of the pinna and cell culture conditions were set to derive and enrich the self renewing cells for further characterisation. The cells were subjected to survival and growth examinations in vitro, and expression of several stemness factors was studied in these cells using reverse transcription polymerase chain reaction (RT-PCR). Results revealed that the derived cells are rather immortal, as they have been growing for more than 120 passages in culture up until this report. Furthermore, RT-PCR and flow cytometry analyses showed that these cells express a number of stemness related genes including Oct4 and Sox2. In conclusion, in this study, stem like cells were derived from blastema tissue of rabbit ears for the first time, showing great self renewing capacity, which provides a suitable in vitro model for regeneration studies. Moreover, they could be considered as a good source of stem like cells for future applications.https://jcmr.um.ac.ir/article_26173_50e2df6e04b7078055ab9e9bb43b2aa0.pdfregenerationblastema tissuepluripotencystem cell |
| spellingShingle | Maryam Moghaddam Matin Morvarid Saeinasab Saeideh Nakhaei-Rad Mahdi Mirahmadi Nasser Mahdavi Shahri Mahmoud Mahmoudi Ahmad Reza Bahrami Blastema cells derived from rabbit ear show stem cell characteristics Journal of Cell and Molecular Research regeneration blastema tissue pluripotency stem cell |
| title | Blastema cells derived from rabbit ear show stem cell characteristics |
| title_full | Blastema cells derived from rabbit ear show stem cell characteristics |
| title_fullStr | Blastema cells derived from rabbit ear show stem cell characteristics |
| title_full_unstemmed | Blastema cells derived from rabbit ear show stem cell characteristics |
| title_short | Blastema cells derived from rabbit ear show stem cell characteristics |
| title_sort | blastema cells derived from rabbit ear show stem cell characteristics |
| topic | regeneration blastema tissue pluripotency stem cell |
| url | https://jcmr.um.ac.ir/article_26173_50e2df6e04b7078055ab9e9bb43b2aa0.pdf |
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