Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth
A high pressure apparatus allowing one to study enzyme kinetics under pressure was used to study the self-cleavage activity of the avocado sunblotch viroid. The kinetics of this reaction were determined under pressure over a range up to 300 MPa (1–3000 bar). It appears that the initial rate of this...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/8/720 |
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| author | Hussein Kaddour Honorine Lucchi Guy Hervé Jacques Vergne Marie-Christine Maurel |
| author_facet | Hussein Kaddour Honorine Lucchi Guy Hervé Jacques Vergne Marie-Christine Maurel |
| author_sort | Hussein Kaddour |
| collection | DOAJ |
| description | A high pressure apparatus allowing one to study enzyme kinetics under pressure was used to study the self-cleavage activity of the avocado sunblotch viroid. The kinetics of this reaction were determined under pressure over a range up to 300 MPa (1–3000 bar). It appears that the initial rate of this reaction decreases when pressure increases, revealing a positive ΔV≠ of activation, which correlates with the domain closure accompanying the reaction and the decrease of the surface of the viroid exposed to the solvent. Although, as expected, temperature increases the rate of the reaction whose energy of activation was determined, it appeared that it does not significantly influence the ΔV≠ of activation and that pressure does not influence the energy of activation. These results provide information about the structural aspects or this self-cleavage reaction, which is involved in the process of maturation of this viroid. The behavior of ASBVd results from the involvement of the hammerhead ribozyme present at its catalytic domain, indeed a structural motif is very widespread in the ancient and current RNA world. |
| first_indexed | 2024-03-10T09:00:02Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T09:00:02Z |
| publishDate | 2021-07-01 |
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| series | Biology |
| spelling | doaj.art-14983a09b73b461a84cf95d882c6a62f2023-11-22T06:49:34ZengMDPI AGBiology2079-77372021-07-0110872010.3390/biology10080720Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on EarthHussein Kaddour0Honorine Lucchi1Guy Hervé2Jacques Vergne3Marie-Christine Maurel4Department of pharmacology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY 11794, USASociété PYMABS, 5 rue Henri Auguste Desbyeres, 91000 Évry-Courcouronnes, FranceLaboratoire BIOSIPE, Institut de biologie Paris-Seine, Sorbonne Université, 7 quai Saint-Bernard, 75005 Paris, FranceInstitut de Systématique, Evolution, Biodiversité, (ISYEB), Sorbonne Université, Museum National d’Histoire Naturelle, CNRS, EPHE, F 75005 Paris, FranceInstitut de Systématique, Evolution, Biodiversité, (ISYEB), Sorbonne Université, Museum National d’Histoire Naturelle, CNRS, EPHE, F 75005 Paris, FranceA high pressure apparatus allowing one to study enzyme kinetics under pressure was used to study the self-cleavage activity of the avocado sunblotch viroid. The kinetics of this reaction were determined under pressure over a range up to 300 MPa (1–3000 bar). It appears that the initial rate of this reaction decreases when pressure increases, revealing a positive ΔV≠ of activation, which correlates with the domain closure accompanying the reaction and the decrease of the surface of the viroid exposed to the solvent. Although, as expected, temperature increases the rate of the reaction whose energy of activation was determined, it appeared that it does not significantly influence the ΔV≠ of activation and that pressure does not influence the energy of activation. These results provide information about the structural aspects or this self-cleavage reaction, which is involved in the process of maturation of this viroid. The behavior of ASBVd results from the involvement of the hammerhead ribozyme present at its catalytic domain, indeed a structural motif is very widespread in the ancient and current RNA world.https://www.mdpi.com/2079-7737/10/8/720viroidhydrostatic pressuretemperaturestructure–activity relationshipRNA World |
| spellingShingle | Hussein Kaddour Honorine Lucchi Guy Hervé Jacques Vergne Marie-Christine Maurel Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth Biology viroid hydrostatic pressure temperature structure–activity relationship RNA World |
| title | Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth |
| title_full | Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth |
| title_fullStr | Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth |
| title_full_unstemmed | Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth |
| title_short | Kinetic Study of the Avocado Sunblotch Viroid Self-Cleavage Reaction Reveals Compensatory Effects between High-Pressure and High-Temperature: Implications for Origins of Life on Earth |
| title_sort | kinetic study of the avocado sunblotch viroid self cleavage reaction reveals compensatory effects between high pressure and high temperature implications for origins of life on earth |
| topic | viroid hydrostatic pressure temperature structure–activity relationship RNA World |
| url | https://www.mdpi.com/2079-7737/10/8/720 |
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