Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot
During storage and transportation after harvest, the jujube fruit is susceptible to black spot rot, which is caused by <i>Alternaria alternata</i>. The present study aimed to evaluate the effectiveness of the yeast <i>Meyerozyma caribbica</i> in controlling <i>A. altern...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/12/1313 |
| _version_ | 1827638156540772352 |
|---|---|
| author | Qian Deng Xingmeng Lei Hongyan Zhang Lili Deng Lanhua Yi Kaifang Zeng |
| author_facet | Qian Deng Xingmeng Lei Hongyan Zhang Lili Deng Lanhua Yi Kaifang Zeng |
| author_sort | Qian Deng |
| collection | DOAJ |
| description | During storage and transportation after harvest, the jujube fruit is susceptible to black spot rot, which is caused by <i>Alternaria alternata</i>. The present study aimed to evaluate the effectiveness of the yeast <i>Meyerozyma caribbica</i> in controlling <i>A. alternata</i> in postharvest jujube fruits, and to explore the biofilm formation mechanism. The results showed that <i>M. caribbica</i> treatment significantly reduced the <i>A. alternata</i> decay in jujube fruits. <i>M. caribbica</i> could rapidly colonize jujube fruit wounds, adhering tightly to hyphae of <i>A. alternata</i>, and accompanied by the production of extracellular secretions. In <i>in vitro</i> experiments, we identified that <i>M. caribbica</i> adhered to polystyrene plates, indicating a strong biofilm-forming ability. Furthermore, we demonstrated that <i>M. caribbica</i> can secrete phenylethanol, a quorum sensing molecule which can affect biofilm development. Phenylalanine (a precursor substance for phenylethanol synthesis) enhanced the secretion of phenylethanol and promoted the formation of <i>M. caribbica</i> biofilms. Meanwhile, phenylalanine enhanced the biological control performance of <i>M. caribbica</i> against jujube black spot rot. Our study provided new insights that enhance the biological control performance of antagonistic yeast. |
| first_indexed | 2024-03-09T16:13:34Z |
| format | Article |
| id | doaj.art-149b544737db4b2fa2a83fd95fa1f8a3 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:13:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-149b544737db4b2fa2a83fd95fa1f8a32023-11-24T16:00:20ZengMDPI AGJournal of Fungi2309-608X2022-12-01812131310.3390/jof8121313Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot RotQian Deng0Xingmeng Lei1Hongyan Zhang2Lili Deng3Lanhua Yi4Kaifang Zeng5College of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaDuring storage and transportation after harvest, the jujube fruit is susceptible to black spot rot, which is caused by <i>Alternaria alternata</i>. The present study aimed to evaluate the effectiveness of the yeast <i>Meyerozyma caribbica</i> in controlling <i>A. alternata</i> in postharvest jujube fruits, and to explore the biofilm formation mechanism. The results showed that <i>M. caribbica</i> treatment significantly reduced the <i>A. alternata</i> decay in jujube fruits. <i>M. caribbica</i> could rapidly colonize jujube fruit wounds, adhering tightly to hyphae of <i>A. alternata</i>, and accompanied by the production of extracellular secretions. In <i>in vitro</i> experiments, we identified that <i>M. caribbica</i> adhered to polystyrene plates, indicating a strong biofilm-forming ability. Furthermore, we demonstrated that <i>M. caribbica</i> can secrete phenylethanol, a quorum sensing molecule which can affect biofilm development. Phenylalanine (a precursor substance for phenylethanol synthesis) enhanced the secretion of phenylethanol and promoted the formation of <i>M. caribbica</i> biofilms. Meanwhile, phenylalanine enhanced the biological control performance of <i>M. caribbica</i> against jujube black spot rot. Our study provided new insights that enhance the biological control performance of antagonistic yeast.https://www.mdpi.com/2309-608X/8/12/1313<i>Meyerozyma caribbica</i>jujubeblack spot rotbiofilm formationphenylalanine |
| spellingShingle | Qian Deng Xingmeng Lei Hongyan Zhang Lili Deng Lanhua Yi Kaifang Zeng Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot Journal of Fungi <i>Meyerozyma caribbica</i> jujube black spot rot biofilm formation phenylalanine |
| title | Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot |
| title_full | Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot |
| title_fullStr | Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot |
| title_full_unstemmed | Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot |
| title_short | Phenylalanine Promotes Biofilm Formation of <i>Meyerozyma caribbica</i> to Improve Biocontrol Efficacy against Jujube Black Spot Rot |
| title_sort | phenylalanine promotes biofilm formation of i meyerozyma caribbica i to improve biocontrol efficacy against jujube black spot rot |
| topic | <i>Meyerozyma caribbica</i> jujube black spot rot biofilm formation phenylalanine |
| url | https://www.mdpi.com/2309-608X/8/12/1313 |
| work_keys_str_mv | AT qiandeng phenylalaninepromotesbiofilmformationofimeyerozymacaribbicaitoimprovebiocontrolefficacyagainstjujubeblackspotrot AT xingmenglei phenylalaninepromotesbiofilmformationofimeyerozymacaribbicaitoimprovebiocontrolefficacyagainstjujubeblackspotrot AT hongyanzhang phenylalaninepromotesbiofilmformationofimeyerozymacaribbicaitoimprovebiocontrolefficacyagainstjujubeblackspotrot AT lilideng phenylalaninepromotesbiofilmformationofimeyerozymacaribbicaitoimprovebiocontrolefficacyagainstjujubeblackspotrot AT lanhuayi phenylalaninepromotesbiofilmformationofimeyerozymacaribbicaitoimprovebiocontrolefficacyagainstjujubeblackspotrot AT kaifangzeng phenylalaninepromotesbiofilmformationofimeyerozymacaribbicaitoimprovebiocontrolefficacyagainstjujubeblackspotrot |