Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.)
Low temperature (cold) and freezing stress is a major problem during winter wheat growth. Low temperature tolerance (LT) is an important agronomic trait in winter wheat and determines the plants’ ability to cope with below-freezing temperatures; thus, the development of cold-tolerant cultivars has b...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/8/1641 |
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| author | Parisa Bolouri Kamil Haliloğlu Seyyed Abolghasem Mohammadi Aras Türkoğlu Emre İlhan Gniewko Niedbała Piotr Szulc Mohsen Niazian |
| author_facet | Parisa Bolouri Kamil Haliloğlu Seyyed Abolghasem Mohammadi Aras Türkoğlu Emre İlhan Gniewko Niedbała Piotr Szulc Mohsen Niazian |
| author_sort | Parisa Bolouri |
| collection | DOAJ |
| description | Low temperature (cold) and freezing stress is a major problem during winter wheat growth. Low temperature tolerance (LT) is an important agronomic trait in winter wheat and determines the plants’ ability to cope with below-freezing temperatures; thus, the development of cold-tolerant cultivars has become a major goal of breeding in various regions of the world. In this study, we sought to identify quantitative trait loci (QTL) using molecular markers related to freezing tolerance in winter. Thirty-four polymorphic markers among 425 SSR markers were obtained for the population, including 180 inbred lines of F<sub>12</sub> generation wheat, derived from crosses (Norstar × Zagros) after testing with parents. LT<sub>50</sub> is used as an effective selection criterion for identifying frost-tolerance genotypes. The progeny of individual F<sub>12</sub> plants were used to evaluate LT50. Several QTLs related to wheat yield, including heading time period, 1000-seed weight, and number of surviving plants after overwintering, were identified. Single-marker analysis illustrated that four SSR markers with a total of 25% phenotypic variance determination were linked to LT50. Related QTLs were located on chromosomes 4A, 2B, and 3B. Common QTLs identified in two cropping seasons based on agronomical traits were two QTLs for heading time period, one QTL for 1000-seed weight, and six QTLs for number of surviving plants after overwintering. The four markers identified linked to LT<sub>50</sub> significantly affected both LT<sub>50</sub> and yield-related traits simultaneously. This is the first report to identify a major-effect QTL related to frost tolerance on chromosome 4A by the marker XGWM160. It is possible that some QTLs are closely related to pleiotropic effects that control two or more traits simultaneously, and this feature can be used as a factor to select frost-resistant lines in plant breeding programs. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:35:38Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-149bc5f986b244949cf50cdef631a6462023-11-17T20:59:34ZengMDPI AGPlants2223-77472023-04-01128164110.3390/plants12081641Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.)Parisa Bolouri0Kamil Haliloğlu1Seyyed Abolghasem Mohammadi2Aras Türkoğlu3Emre İlhan4Gniewko Niedbała5Piotr Szulc6Mohsen Niazian7Department of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TurkeyDepartment of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TurkeyDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 5166616471, IranDepartment of Field Crops, Faculty of Agriculture, Necmettin Erbakan University, 42310 Konya, TurkeyDepartment of Molecular Biology and Genetics, Erzurum Technical University, 25240 Erzurum, TurkeyDepartment of Biosystems Engineering, Faculty of Environmental and Mechanical Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-627 Poznań, PolandDepartment of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, PolandField and Horticultural Crops Research Department, Kurdistan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sanandaj 6616936311, IranLow temperature (cold) and freezing stress is a major problem during winter wheat growth. Low temperature tolerance (LT) is an important agronomic trait in winter wheat and determines the plants’ ability to cope with below-freezing temperatures; thus, the development of cold-tolerant cultivars has become a major goal of breeding in various regions of the world. In this study, we sought to identify quantitative trait loci (QTL) using molecular markers related to freezing tolerance in winter. Thirty-four polymorphic markers among 425 SSR markers were obtained for the population, including 180 inbred lines of F<sub>12</sub> generation wheat, derived from crosses (Norstar × Zagros) after testing with parents. LT<sub>50</sub> is used as an effective selection criterion for identifying frost-tolerance genotypes. The progeny of individual F<sub>12</sub> plants were used to evaluate LT50. Several QTLs related to wheat yield, including heading time period, 1000-seed weight, and number of surviving plants after overwintering, were identified. Single-marker analysis illustrated that four SSR markers with a total of 25% phenotypic variance determination were linked to LT50. Related QTLs were located on chromosomes 4A, 2B, and 3B. Common QTLs identified in two cropping seasons based on agronomical traits were two QTLs for heading time period, one QTL for 1000-seed weight, and six QTLs for number of surviving plants after overwintering. The four markers identified linked to LT<sub>50</sub> significantly affected both LT<sub>50</sub> and yield-related traits simultaneously. This is the first report to identify a major-effect QTL related to frost tolerance on chromosome 4A by the marker XGWM160. It is possible that some QTLs are closely related to pleiotropic effects that control two or more traits simultaneously, and this feature can be used as a factor to select frost-resistant lines in plant breeding programs.https://www.mdpi.com/2223-7747/12/8/1641winter wheatfrost toleranceLT<sub>50</sub>chromosome 4ASSR markeryield-related traits |
| spellingShingle | Parisa Bolouri Kamil Haliloğlu Seyyed Abolghasem Mohammadi Aras Türkoğlu Emre İlhan Gniewko Niedbała Piotr Szulc Mohsen Niazian Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.) Plants winter wheat frost tolerance LT<sub>50</sub> chromosome 4A SSR marker yield-related traits |
| title | Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.) |
| title_full | Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.) |
| title_fullStr | Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.) |
| title_full_unstemmed | Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.) |
| title_short | Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (<i>Triticum aestivum</i> L.) |
| title_sort | identification of novel qtls associated with frost tolerance in winter wheat i triticum aestivum i l |
| topic | winter wheat frost tolerance LT<sub>50</sub> chromosome 4A SSR marker yield-related traits |
| url | https://www.mdpi.com/2223-7747/12/8/1641 |
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