Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics
To better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after <i>in ovo</i> administrati...
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MDPI AG
2020-04-01
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| Cyfres: | Animals |
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| Mynediad Ar-lein: | https://www.mdpi.com/2076-2615/10/4/687 |
| _version_ | 1827718593209434112 |
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| author | Aleksandra Dunislawska Maria Siwek Anna Slawinska Adam Lepczynski Agnieszka Herosimczyk Pawel A. Kolodziejski Marek Bednarczyk |
| author_facet | Aleksandra Dunislawska Maria Siwek Anna Slawinska Adam Lepczynski Agnieszka Herosimczyk Pawel A. Kolodziejski Marek Bednarczyk |
| author_sort | Aleksandra Dunislawska |
| collection | DOAJ |
| description | To better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after <i>in ovo</i> administration of synbiotics remains to be reported. The purpose of the present study was to analyze the molecular background of meat quality based on gene expression and basic physiological parameters. Eggs were injected with (S1) <i>Lactobacillus salivarius</i> with galacto-oligosaccharides or (S2) <i>Lactobacillus plantarum</i> with raffinose family oligosaccharides. The pectoral muscle was collected at two time points (day 7 and day 42) and subjected to RNA isolation. Gene expression analysis was performed by RT-qPCR for a panel of eight genes associated with metabolism. The concentration of glucose and hormones (insulin, glucagon, and leptin (S1 <i>p</i> = 0.04)) was also increased. The obtained results showed that metabolic gene expression in the muscle was more differential due to synbiotic stimulation on day 7 (<i>FST</i> in S1 <i>p</i> = 0.03; <i>PDK4</i> in S1 <i>p</i> = 0.02 and S2 <i>p</i> = 0.01; <i>CEBPB</i> in S1 <i>p</i> = 0.01 and S2 <i>p =</i> 0.008; PHKB in S1 <i>p =</i> 0.01; <i>PRKAG3</i> in S1 <i>p =</i> 0.02) than on day 42 (<i>PDK4</i> in S1 <i>p =</i> 0.04). On the basis of the results obtained, it can be concluded that <i>in ovo</i> stimulation with S1 triggered the most potent and favorable changes in the pectoral muscle gene expression in broiler chickens. |
| first_indexed | 2024-03-10T20:27:45Z |
| format | Article |
| id | doaj.art-149c756066ff4114a01b5134e881f1e9 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:27:45Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-149c756066ff4114a01b5134e881f1e92023-11-19T21:40:01ZengMDPI AGAnimals2076-26152020-04-0110468710.3390/ani10040687Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with SynbioticsAleksandra Dunislawska0Maria Siwek1Anna Slawinska2Adam Lepczynski3Agnieszka Herosimczyk4Pawel A. Kolodziejski5Marek Bednarczyk6UTP, Department of Animal Biotechnology and Genetics, University of Science and Technology, 85-084 Bydgoszcz, PolandUTP, Department of Animal Biotechnology and Genetics, University of Science and Technology, 85-084 Bydgoszcz, PolandUTP, Department of Animal Biotechnology and Genetics, University of Science and Technology, 85-084 Bydgoszcz, PolandDepartment of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology, 71-270 Szczecin, PolandDepartment of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology, 71-270 Szczecin, PolandDepartment of Animal Physiology and Biochemistry, Poznan University of Life Sciences, 60-637 Poznan, PolandUTP, Department of Animal Biotechnology and Genetics, University of Science and Technology, 85-084 Bydgoszcz, PolandTo better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after <i>in ovo</i> administration of synbiotics remains to be reported. The purpose of the present study was to analyze the molecular background of meat quality based on gene expression and basic physiological parameters. Eggs were injected with (S1) <i>Lactobacillus salivarius</i> with galacto-oligosaccharides or (S2) <i>Lactobacillus plantarum</i> with raffinose family oligosaccharides. The pectoral muscle was collected at two time points (day 7 and day 42) and subjected to RNA isolation. Gene expression analysis was performed by RT-qPCR for a panel of eight genes associated with metabolism. The concentration of glucose and hormones (insulin, glucagon, and leptin (S1 <i>p</i> = 0.04)) was also increased. The obtained results showed that metabolic gene expression in the muscle was more differential due to synbiotic stimulation on day 7 (<i>FST</i> in S1 <i>p</i> = 0.03; <i>PDK4</i> in S1 <i>p</i> = 0.02 and S2 <i>p</i> = 0.01; <i>CEBPB</i> in S1 <i>p</i> = 0.01 and S2 <i>p =</i> 0.008; PHKB in S1 <i>p =</i> 0.01; <i>PRKAG3</i> in S1 <i>p =</i> 0.02) than on day 42 (<i>PDK4</i> in S1 <i>p =</i> 0.04). On the basis of the results obtained, it can be concluded that <i>in ovo</i> stimulation with S1 triggered the most potent and favorable changes in the pectoral muscle gene expression in broiler chickens.https://www.mdpi.com/2076-2615/10/4/687in ovo technologypoultrymeatsynbioticsgene expression |
| spellingShingle | Aleksandra Dunislawska Maria Siwek Anna Slawinska Adam Lepczynski Agnieszka Herosimczyk Pawel A. Kolodziejski Marek Bednarczyk Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics Animals in ovo technology poultry meat synbiotics gene expression |
| title | Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics |
| title_full | Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics |
| title_fullStr | Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics |
| title_full_unstemmed | Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics |
| title_short | Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics |
| title_sort | metabolic gene expression in the muscle and blood parameters of broiler chickens stimulated in ovo with synbiotics |
| topic | in ovo technology poultry meat synbiotics gene expression |
| url | https://www.mdpi.com/2076-2615/10/4/687 |
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