Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System
Stimuli-responsive hydrogel matrices are inspiring manifold applications in controlled delivery of bioactive compounds. Elastin-derived polypeptides form hydrogel matrices that may release bioactive moieties as a function of local increase of active elastases, as it would occur in several processes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2018.00127/full |
| _version_ | 1818529029346557952 |
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| author | Antonella Bandiera Sabina Passamonti Luisa Stella Dolci Maria Letizia Focarete Maria Letizia Focarete |
| author_facet | Antonella Bandiera Sabina Passamonti Luisa Stella Dolci Maria Letizia Focarete Maria Letizia Focarete |
| author_sort | Antonella Bandiera |
| collection | DOAJ |
| description | Stimuli-responsive hydrogel matrices are inspiring manifold applications in controlled delivery of bioactive compounds. Elastin-derived polypeptides form hydrogel matrices that may release bioactive moieties as a function of local increase of active elastases, as it would occur in several processes like inflammation. In view of the development of a patch for healing wounds, recombinant elastin-based polypeptides were combined with a proteolysis-resistant scaffold, made of electrospun poly-L-lactic acid (PLLA) fibers. The results of this study demonstrated the compatibility of these two components. An efficient procedure to obtain a composite material retaining the main features of each component was established. The release of the elastin moiety was monitored by means of a simple protocol. Our data showed that electrospun PLLA can form a composite with fusion proteins bound to elastin-derived polypeptides. Therefore, our approach allows designing a therapeutic agent delivery platform to realize devices capable of responding and interacting with biological systems at the molecular level. |
| first_indexed | 2024-12-11T06:57:47Z |
| format | Article |
| id | doaj.art-14a0035080aa4a6cbbcdafe4da23a49d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T06:57:47Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-14a0035080aa4a6cbbcdafe4da23a49d2022-12-22T01:16:42ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-09-01610.3389/fbioe.2018.00127413589Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery SystemAntonella Bandiera0Sabina Passamonti1Luisa Stella Dolci2Maria Letizia Focarete3Maria Letizia Focarete4Department of Life Sciences, University of Trieste, Trieste, ItalyDepartment of Life Sciences, University of Trieste, Trieste, ItalyDepartment of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, Bologna RU), Alma Mater Studiorum - Università di Bologna, Bologna, ItalyDepartment of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, Bologna RU), Alma Mater Studiorum - Università di Bologna, Bologna, ItalyHealth Sciences and Technologies–Interdepartmental Center for Industrial Research, Alma Mater Studiorum - Università di Bologna, Bologna, ItalyStimuli-responsive hydrogel matrices are inspiring manifold applications in controlled delivery of bioactive compounds. Elastin-derived polypeptides form hydrogel matrices that may release bioactive moieties as a function of local increase of active elastases, as it would occur in several processes like inflammation. In view of the development of a patch for healing wounds, recombinant elastin-based polypeptides were combined with a proteolysis-resistant scaffold, made of electrospun poly-L-lactic acid (PLLA) fibers. The results of this study demonstrated the compatibility of these two components. An efficient procedure to obtain a composite material retaining the main features of each component was established. The release of the elastin moiety was monitored by means of a simple protocol. Our data showed that electrospun PLLA can form a composite with fusion proteins bound to elastin-derived polypeptides. Therefore, our approach allows designing a therapeutic agent delivery platform to realize devices capable of responding and interacting with biological systems at the molecular level.https://www.frontiersin.org/article/10.3389/fbioe.2018.00127/fullelastinelectrospun matrixcompositesmart releasedrug delivery |
| spellingShingle | Antonella Bandiera Sabina Passamonti Luisa Stella Dolci Maria Letizia Focarete Maria Letizia Focarete Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System Frontiers in Bioengineering and Biotechnology elastin electrospun matrix composite smart release drug delivery |
| title | Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System |
| title_full | Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System |
| title_fullStr | Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System |
| title_full_unstemmed | Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System |
| title_short | Composite of Elastin-Based Matrix and Electrospun Poly(L-Lactic Acid) Fibers: A Potential Smart Drug Delivery System |
| title_sort | composite of elastin based matrix and electrospun poly l lactic acid fibers a potential smart drug delivery system |
| topic | elastin electrospun matrix composite smart release drug delivery |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2018.00127/full |
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