Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches
Abstract The purpose of this study is to evaluate the likely defensive impact of Ajwa date aqueous extract (AJDAE) in alleviating the nephrotoxicity generated by doxorubicin (DOX) injection in rats. Sixty male Wister albino rats were randomly and equally separated into six groups (n = 10), and they...
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| Format: | Article |
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Wiley
2023-03-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3199 |
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| author | Othman A. S. Baothman Hisham N. Altayb Mustafa A. Zeyadi Salman B. Hosawi Mohamed Kamel Abo‐Golayel |
| author_facet | Othman A. S. Baothman Hisham N. Altayb Mustafa A. Zeyadi Salman B. Hosawi Mohamed Kamel Abo‐Golayel |
| author_sort | Othman A. S. Baothman |
| collection | DOAJ |
| description | Abstract The purpose of this study is to evaluate the likely defensive impact of Ajwa date aqueous extract (AJDAE) in alleviating the nephrotoxicity generated by doxorubicin (DOX) injection in rats. Sixty male Wister albino rats were randomly and equally separated into six groups (n = 10), and they were treated as follows: untreated control group, extract groups administered with 0.75 and 1.5 mg kg bw of AJDAE, toxicant control group administered with DOX, and prophylactic groups were treated with 0.75 and 1.5 mg/kg of AJDAE and 15 mg/kg DOX. Biochemical parameters, antioxidant enzymes, renal functions, DNA integrity, and histopathology were studied to evaluate the nephroprotective activity of AJDAE. Furthermore, bioactive compounds were utilized for in silico molecular docking. AJDAE treatment resulted in significant improvements in the amended renal biomarkers (urea, creatinine, calcium, phosphorous, and uric acid), antioxidative markers, and MDA. Noticeable histopathological improvements supported this result. Results of in silico studies revealed that d‐Mannitol, 6TMS derivative, palmitic acid, and TMS derivative had a higher docking score with human soluble epoxide hydrolase (−10.9 kcal/mol) and NF‐κB‐DNA (−7 kcal/mol). The present findings indicated that AJDAE could decrease ROS generation and lipid peroxidation (LPO) and repair the DOX injection‐related DNA damage. |
| first_indexed | 2024-04-10T04:24:40Z |
| format | Article |
| id | doaj.art-14a13cb4ae7b42678fc3b6b13eb6a6d7 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-10T04:24:40Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-14a13cb4ae7b42678fc3b6b13eb6a6d72023-03-10T17:16:14ZengWileyFood Science & Nutrition2048-71772023-03-011131584159810.1002/fsn3.3199Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approachesOthman A. S. Baothman0Hisham N. Altayb1Mustafa A. Zeyadi2Salman B. Hosawi3Mohamed Kamel Abo‐Golayel4Biochemistry Department, Faculty of Science King Abdulaziz University Jeddah Saudi ArabiaBiochemistry Department, Faculty of Science King Abdulaziz University Jeddah Saudi ArabiaBiochemistry Department, Faculty of Science King Abdulaziz University Jeddah Saudi ArabiaBiochemistry Department, Faculty of Science King Abdulaziz University Jeddah Saudi ArabiaBiochemistry Department, Faculty of Science King Abdulaziz University Jeddah Saudi ArabiaAbstract The purpose of this study is to evaluate the likely defensive impact of Ajwa date aqueous extract (AJDAE) in alleviating the nephrotoxicity generated by doxorubicin (DOX) injection in rats. Sixty male Wister albino rats were randomly and equally separated into six groups (n = 10), and they were treated as follows: untreated control group, extract groups administered with 0.75 and 1.5 mg kg bw of AJDAE, toxicant control group administered with DOX, and prophylactic groups were treated with 0.75 and 1.5 mg/kg of AJDAE and 15 mg/kg DOX. Biochemical parameters, antioxidant enzymes, renal functions, DNA integrity, and histopathology were studied to evaluate the nephroprotective activity of AJDAE. Furthermore, bioactive compounds were utilized for in silico molecular docking. AJDAE treatment resulted in significant improvements in the amended renal biomarkers (urea, creatinine, calcium, phosphorous, and uric acid), antioxidative markers, and MDA. Noticeable histopathological improvements supported this result. Results of in silico studies revealed that d‐Mannitol, 6TMS derivative, palmitic acid, and TMS derivative had a higher docking score with human soluble epoxide hydrolase (−10.9 kcal/mol) and NF‐κB‐DNA (−7 kcal/mol). The present findings indicated that AJDAE could decrease ROS generation and lipid peroxidation (LPO) and repair the DOX injection‐related DNA damage.https://doi.org/10.1002/fsn3.3199Ajwa datedoxorubicinmolecular dockingnephroprotectivenephrotoxicityphytochemical |
| spellingShingle | Othman A. S. Baothman Hisham N. Altayb Mustafa A. Zeyadi Salman B. Hosawi Mohamed Kamel Abo‐Golayel Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches Food Science & Nutrition Ajwa date doxorubicin molecular docking nephroprotective nephrotoxicity phytochemical |
| title | Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches |
| title_full | Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches |
| title_fullStr | Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches |
| title_full_unstemmed | Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches |
| title_short | Phytochemical analysis and nephroprotective potential of Ajwa date in doxorubicin‐induced nephrotoxicity rats: Biochemical and molecular docking approaches |
| title_sort | phytochemical analysis and nephroprotective potential of ajwa date in doxorubicin induced nephrotoxicity rats biochemical and molecular docking approaches |
| topic | Ajwa date doxorubicin molecular docking nephroprotective nephrotoxicity phytochemical |
| url | https://doi.org/10.1002/fsn3.3199 |
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