Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea
Hypercholesterolemia is a condition characterized by increased levels of total cholesterol in the blood. Pheophytin is the result of degradation of chlorophyll which is the dominant pigment in green tea, and has been shown to have the same antioxidant properties as ?- carotene. This study aims to de...
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| Format: | Article |
| Language: | English |
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Mulawarman University
2020-06-01
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| Series: | Journal of Tropical Pharmacy and Chemistry |
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| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/222 |
| _version_ | 1827771065276825600 |
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| author | Lia Kusmita Ika Puspitaningrum |
| author_facet | Lia Kusmita Ika Puspitaningrum |
| author_sort | Lia Kusmita |
| collection | DOAJ |
| description | Hypercholesterolemia is a condition characterized by increased levels of total cholesterol in the blood. Pheophytin is the result of degradation of chlorophyll which is the dominant pigment in green tea, and has been shown to have the same antioxidant properties as ?- carotene. This study aims to determine the antihypercholesterolemia effect and effective dose of pheophytin isolates and acute toxicity tests to determine the safety level of pheophytin isolates.
Antihipocolesterolemia test uses induction of high-fat feed that is a mixture of standard food, pork oil and quail egg yolk (16: 3: 1). While the acute toxicity test is carried out based on OECD 423, Clinical symptoms that arise for 3 hours are observed intensively. Histopathological examination data of liver and kidney organs were performed on day 14. Data obtained in the form of LD 50 and clinical symptoms that arise as well as histopathological results were analyzed qualitatively.
Statistical test results of percent reduction in total cholesterol levels showed a significant difference between the negative control group and the pheophytin isolates of the three doses. This proves that pheophytin isolates can have antihypercholesterolemic effect with an effective dose of 10 mg/kgBW. Histopathological examination results of aorta and liver of rats showed that the higher the dose of pheophytin isolates, the lighter the degree of fatty acid. While the toxicity test results showed that the LD50 value of pheophytin isolates was more than 2000 mg / kgBW (non-toxic category). However, pheophytin isolate with LD50 is more than 2000 mg/kgBW which has shown the presence of pancreatic degeneration and hydropic degeneration, but necrosis has not occurred yet.
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| first_indexed | 2024-03-11T12:48:22Z |
| format | Article |
| id | doaj.art-14a30562aa8844148efe284efafc0bfb |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:48:22Z |
| publishDate | 2020-06-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-14a30562aa8844148efe284efafc0bfb2023-11-04T06:08:31ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902020-06-015110.25026/jtpc.v5i1.222Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green TeaLia Kusmita0Ika Puspitaningrum1STIFAR"Yayasan Pharmasi Semarang"STIFAR "Yayasan Pharmasi Semarang"Hypercholesterolemia is a condition characterized by increased levels of total cholesterol in the blood. Pheophytin is the result of degradation of chlorophyll which is the dominant pigment in green tea, and has been shown to have the same antioxidant properties as ?- carotene. This study aims to determine the antihypercholesterolemia effect and effective dose of pheophytin isolates and acute toxicity tests to determine the safety level of pheophytin isolates. Antihipocolesterolemia test uses induction of high-fat feed that is a mixture of standard food, pork oil and quail egg yolk (16: 3: 1). While the acute toxicity test is carried out based on OECD 423, Clinical symptoms that arise for 3 hours are observed intensively. Histopathological examination data of liver and kidney organs were performed on day 14. Data obtained in the form of LD 50 and clinical symptoms that arise as well as histopathological results were analyzed qualitatively. Statistical test results of percent reduction in total cholesterol levels showed a significant difference between the negative control group and the pheophytin isolates of the three doses. This proves that pheophytin isolates can have antihypercholesterolemic effect with an effective dose of 10 mg/kgBW. Histopathological examination results of aorta and liver of rats showed that the higher the dose of pheophytin isolates, the lighter the degree of fatty acid. While the toxicity test results showed that the LD50 value of pheophytin isolates was more than 2000 mg / kgBW (non-toxic category). However, pheophytin isolate with LD50 is more than 2000 mg/kgBW which has shown the presence of pancreatic degeneration and hydropic degeneration, but necrosis has not occurred yet. https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/222pheopytingreen teaantihypercholesterolacute toxicity |
| spellingShingle | Lia Kusmita Ika Puspitaningrum Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea Journal of Tropical Pharmacy and Chemistry pheopytin green tea antihypercholesterol acute toxicity |
| title | Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea |
| title_full | Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea |
| title_fullStr | Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea |
| title_full_unstemmed | Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea |
| title_short | Antihipercolesterolemia Effects and Acute Toxicity Test of Pheophytin on Green Tea |
| title_sort | antihipercolesterolemia effects and acute toxicity test of pheophytin on green tea |
| topic | pheopytin green tea antihypercholesterol acute toxicity |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/222 |
| work_keys_str_mv | AT liakusmita antihipercolesterolemiaeffectsandacutetoxicitytestofpheophytinongreentea AT ikapuspitaningrum antihipercolesterolemiaeffectsandacutetoxicitytestofpheophytinongreentea |