Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb
ABSTRACT: The development of the avian wing pattern has been the subject of heated debate due to its special shape. The Suppressor of cytokine signaling 2 (SOCS2) gene encodes a negative regulator of growth hormone (GH) signaling and bone growth and is known to be strongly expressed in the third dig...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124002517 |
| _version_ | 1797226671692054528 |
|---|---|
| author | Xiaoping Li Shanshan Li Shibin Bai Yining Tang Ziqiu Jia Jialong Yin Xiaona Xu Junpeng Zhang David M. Irwin Shuyi Zhang Zhe Wang |
| author_facet | Xiaoping Li Shanshan Li Shibin Bai Yining Tang Ziqiu Jia Jialong Yin Xiaona Xu Junpeng Zhang David M. Irwin Shuyi Zhang Zhe Wang |
| author_sort | Xiaoping Li |
| collection | DOAJ |
| description | ABSTRACT: The development of the avian wing pattern has been the subject of heated debate due to its special shape. The Suppressor of cytokine signaling 2 (SOCS2) gene encodes a negative regulator of growth hormone (GH) signaling and bone growth and is known to be strongly expressed in the third digit of chicken forelimbs. These observations suggest that SOCS2 might regulate the morphology of the avian wing, however, the function of SOCS2 in avian limb development remains unknown. Here, we reexamined SOCS2 expression in successive developmental stages of chicken limb development by in situ hybridization (ISH) and describe extended expression from the posterior of the stypolod to the third digit of the forelimbs. We used the RCAS avian retrovirus to overexpress SOCS2 in the developing chicken limb buds, which resulted in reduced or malformed chicken wings while hindlimbs developed normally. Transcriptome sequencing (mRNA-Seq) revealed changes in expression of genes known to be associated with growth and development in forelimbs with overexpressed SOCS2. This study highlights a pivotal role for SOCS2 during the development of the wing in the chicken and provides new insight into molecular mechanisms regulating avian limb development. |
| first_indexed | 2024-04-24T14:28:37Z |
| format | Article |
| id | doaj.art-14a3a0dba50b40bb8b48c431a1b9d514 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T14:28:37Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-14a3a0dba50b40bb8b48c431a1b9d5142024-04-03T04:25:56ZengElsevierPoultry Science0032-57912024-06-011036103672Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimbXiaoping Li0Shanshan Li1Shibin Bai2Yining Tang3Ziqiu Jia4Jialong Yin5Xiaona Xu6Junpeng Zhang7David M. Irwin8Shuyi Zhang9Zhe Wang10College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario M5S 1A8, CanadaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China; Corresponding author:ABSTRACT: The development of the avian wing pattern has been the subject of heated debate due to its special shape. The Suppressor of cytokine signaling 2 (SOCS2) gene encodes a negative regulator of growth hormone (GH) signaling and bone growth and is known to be strongly expressed in the third digit of chicken forelimbs. These observations suggest that SOCS2 might regulate the morphology of the avian wing, however, the function of SOCS2 in avian limb development remains unknown. Here, we reexamined SOCS2 expression in successive developmental stages of chicken limb development by in situ hybridization (ISH) and describe extended expression from the posterior of the stypolod to the third digit of the forelimbs. We used the RCAS avian retrovirus to overexpress SOCS2 in the developing chicken limb buds, which resulted in reduced or malformed chicken wings while hindlimbs developed normally. Transcriptome sequencing (mRNA-Seq) revealed changes in expression of genes known to be associated with growth and development in forelimbs with overexpressed SOCS2. This study highlights a pivotal role for SOCS2 during the development of the wing in the chicken and provides new insight into molecular mechanisms regulating avian limb development.http://www.sciencedirect.com/science/article/pii/S0032579124002517chicken forelimbSOCS2expression patternoverexpressionmRNA-Seq |
| spellingShingle | Xiaoping Li Shanshan Li Shibin Bai Yining Tang Ziqiu Jia Jialong Yin Xiaona Xu Junpeng Zhang David M. Irwin Shuyi Zhang Zhe Wang Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb Poultry Science chicken forelimb SOCS2 expression pattern overexpression mRNA-Seq |
| title | Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb |
| title_full | Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb |
| title_fullStr | Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb |
| title_full_unstemmed | Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb |
| title_short | Research Note: SOCS2 contributes to reduction of the third digit during development of the chicken forelimb |
| title_sort | research note socs2 contributes to reduction of the third digit during development of the chicken forelimb |
| topic | chicken forelimb SOCS2 expression pattern overexpression mRNA-Seq |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124002517 |
| work_keys_str_mv | AT xiaopingli researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT shanshanli researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT shibinbai researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT yiningtang researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT ziqiujia researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT jialongyin researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT xiaonaxu researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT junpengzhang researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT davidmirwin researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT shuyizhang researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb AT zhewang researchnotesocs2contributestoreductionofthethirddigitduringdevelopmentofthechickenforelimb |