Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches
Abstract Numerous studies have shown that nanosized zeolitic imidazolate framework particles (ZIF-8 NPs) serve as promising vehicles for pH-responsive drug delivery. An understanding of their interaction with serum proteins present in physiological systems will thus be of critical importance. In thi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14630-y |
| _version_ | 1811334606203387904 |
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| author | Ashi Mittal Sona Gandhi Indrajit Roy |
| author_facet | Ashi Mittal Sona Gandhi Indrajit Roy |
| author_sort | Ashi Mittal |
| collection | DOAJ |
| description | Abstract Numerous studies have shown that nanosized zeolitic imidazolate framework particles (ZIF-8 NPs) serve as promising vehicles for pH-responsive drug delivery. An understanding of their interaction with serum proteins present in physiological systems will thus be of critical importance. In this work, monodisperse ZIF-8 NPs with an average size of 60 nm were synthesized at room temperature and characterized for their various physicochemical properties. Bovine serum albumin (BSA) was used as model serum protein for various interaction studies with ZIF-8 NPs. Spectroscopic techniques such as UV–visible and fluorescence spectroscopy indicated the formation of a ground-state complex with a binding constant of the order 103 M−1 and a single binding site. Steady-state and time-resolved fluorescence spectroscopy confirmed the mechanism of quenching to be static. Conformational changes in the secondary structure of BSA were observed using CD and FT-IR spectroscopies. Binding sites were explored using molecular docking studies. |
| first_indexed | 2024-04-13T17:10:35Z |
| format | Article |
| id | doaj.art-14a54fcd682641498159022a24c4bc75 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T17:10:35Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-14a54fcd682641498159022a24c4bc752022-12-22T02:38:17ZengNature PortfolioScientific Reports2045-23222022-06-0112111110.1038/s41598-022-14630-yMechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approachesAshi Mittal0Sona Gandhi1Indrajit Roy2Department of Chemistry, University of DelhiDepartment of Chemistry, University of DelhiDepartment of Chemistry, University of DelhiAbstract Numerous studies have shown that nanosized zeolitic imidazolate framework particles (ZIF-8 NPs) serve as promising vehicles for pH-responsive drug delivery. An understanding of their interaction with serum proteins present in physiological systems will thus be of critical importance. In this work, monodisperse ZIF-8 NPs with an average size of 60 nm were synthesized at room temperature and characterized for their various physicochemical properties. Bovine serum albumin (BSA) was used as model serum protein for various interaction studies with ZIF-8 NPs. Spectroscopic techniques such as UV–visible and fluorescence spectroscopy indicated the formation of a ground-state complex with a binding constant of the order 103 M−1 and a single binding site. Steady-state and time-resolved fluorescence spectroscopy confirmed the mechanism of quenching to be static. Conformational changes in the secondary structure of BSA were observed using CD and FT-IR spectroscopies. Binding sites were explored using molecular docking studies.https://doi.org/10.1038/s41598-022-14630-y |
| spellingShingle | Ashi Mittal Sona Gandhi Indrajit Roy Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches Scientific Reports |
| title | Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches |
| title_full | Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches |
| title_fullStr | Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches |
| title_full_unstemmed | Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches |
| title_short | Mechanistic interaction studies of synthesized ZIF-8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches |
| title_sort | mechanistic interaction studies of synthesized zif 8 nanoparticles with bovine serum albumin using spectroscopic and molecular docking approaches |
| url | https://doi.org/10.1038/s41598-022-14630-y |
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