Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests
Aim: Brucellosis is a disease of zoonotic importance as it affects both human as well as animal’s health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2016-07-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.9/July-2016/11.pdf |
| _version_ | 1818589979197046784 |
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| author | H. K. Sharma S. K. Kotwal D. K. Singh M. A. Malik Arvind Kumar Rajagunalan M. Singh |
| author_facet | H. K. Sharma S. K. Kotwal D. K. Singh M. A. Malik Arvind Kumar Rajagunalan M. Singh |
| author_sort | H. K. Sharma |
| collection | DOAJ |
| description | Aim: Brucellosis is a disease of zoonotic importance as it affects both human as well as animal’s health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas.
Materials and Methods: A total of 121 sera samples from humans occupied with professional related to animals were collected and tested for anti-Brucella antibodies by Rose Bengal plate test (RBPT), modified RBPT (mRBPT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (I-ELISA). Sampling was done keeping in view with the occupation, sex, and age.
Results: The overall seroprevalence of brucellosis recorded was 4.96%. The test-wise seroprevalence was 9.91% by RBPT, 9.91% by mRBPT, 9.09% by STAT, and 16.52% by I-ELISA. The prevalence of brucellosis was higher in >35-50 years age group compared to >20-35 years and >50-65 years. Sex-wise seroprevalence was higher in males than females. Taking I-ELISA as standard, the relative sensitivities of mRBPT, RBPT, and I-ELISA were in the order of mRBPT=RBPT>STAT. All the tests revealed high specificity values; however, among different serological tests, I-ELISA detected a maximum number of positive sera samples.
Conclusions: The prevalence of brucellosis was found to be approximately 5%. The adult (>35-50 years) age male group was most vulnerable. The routine diagnosis of brucellosis involved the conventional serological tests, viz., RBPT and STAT, but each was associated with drawbacks which could give either false-positive or false-negative interpretation. Therefore, it is always recommended to use a battery of tests in the diagnosis of brucellosis. |
| first_indexed | 2024-12-16T09:49:15Z |
| format | Article |
| id | doaj.art-14a5ca49311c4d52a0d63d2d1143dc57 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T09:49:15Z |
| publishDate | 2016-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-14a5ca49311c4d52a0d63d2d1143dc572022-12-21T22:36:07ZengVeterinary WorldVeterinary World0972-89882231-09162016-07-019774274610.14202/vetworld.2016.742-746Seroprevalence of human brucellosis in and around Jammu, India, using different serological testsH. K. Sharma0S. K. Kotwal1D. K. Singh2M. A. Malik3Arvind Kumar4Rajagunalan5M. Singh6Division of Veterinary Public Health and Epidemiology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India; harshvphe@gmail.comDivision of Veterinary Public Health and Epidemiology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India; kotwalsk@yahoo.co.inDivision of Veterinary Public Health, Brucella Laboratory, Indian Veterinary Research Institute, Izzatnagar, Bareilly, Uttar Pradesh, India; dks_bly@yahoo.comDivision of Veterinary Public Health and Epidemiology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India; malikskuast@yahoo.comDivision of Livestock Products Technology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India; drarvindlpt@gmail.comDivision of Veterinary Public Health, Brucella Laboratory, Indian Veterinary Research Institute, Izzatnagar, Bareilly, Uttar Pradesh, India; drgunavet@gmail.comDivision of Veterinary Public Health and Epidemiology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India; manindersingh2k2@gmail.comAim: Brucellosis is a disease of zoonotic importance as it affects both human as well as animal’s health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas. Materials and Methods: A total of 121 sera samples from humans occupied with professional related to animals were collected and tested for anti-Brucella antibodies by Rose Bengal plate test (RBPT), modified RBPT (mRBPT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (I-ELISA). Sampling was done keeping in view with the occupation, sex, and age. Results: The overall seroprevalence of brucellosis recorded was 4.96%. The test-wise seroprevalence was 9.91% by RBPT, 9.91% by mRBPT, 9.09% by STAT, and 16.52% by I-ELISA. The prevalence of brucellosis was higher in >35-50 years age group compared to >20-35 years and >50-65 years. Sex-wise seroprevalence was higher in males than females. Taking I-ELISA as standard, the relative sensitivities of mRBPT, RBPT, and I-ELISA were in the order of mRBPT=RBPT>STAT. All the tests revealed high specificity values; however, among different serological tests, I-ELISA detected a maximum number of positive sera samples. Conclusions: The prevalence of brucellosis was found to be approximately 5%. The adult (>35-50 years) age male group was most vulnerable. The routine diagnosis of brucellosis involved the conventional serological tests, viz., RBPT and STAT, but each was associated with drawbacks which could give either false-positive or false-negative interpretation. Therefore, it is always recommended to use a battery of tests in the diagnosis of brucellosis.http://www.veterinaryworld.org/Vol.9/July-2016/11.pdfbrucellosishumansindirect enzyme-linked immunosorbent assayRose Bengal plate testseroprevalencestandard tube agglutination test |
| spellingShingle | H. K. Sharma S. K. Kotwal D. K. Singh M. A. Malik Arvind Kumar Rajagunalan M. Singh Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests Veterinary World brucellosis humans indirect enzyme-linked immunosorbent assay Rose Bengal plate test seroprevalence standard tube agglutination test |
| title | Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests |
| title_full | Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests |
| title_fullStr | Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests |
| title_full_unstemmed | Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests |
| title_short | Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests |
| title_sort | seroprevalence of human brucellosis in and around jammu india using different serological tests |
| topic | brucellosis humans indirect enzyme-linked immunosorbent assay Rose Bengal plate test seroprevalence standard tube agglutination test |
| url | http://www.veterinaryworld.org/Vol.9/July-2016/11.pdf |
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