The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist.
Fibrobacter succinogenes is an important member of the rumen microbial community that converts plant biomass into nutrients usable by its host. This bacterium, which is also one of only two cultivated species in its phylum, is an efficient and prolific degrader of cellulose. Specifically, it has a p...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3079729?pdf=render |
| _version_ | 1811218969394151424 |
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| author | Garret Suen Paul J Weimer David M Stevenson Frank O Aylward Julie Boyum Jan Deneke Colleen Drinkwater Natalia N Ivanova Natalia Mikhailova Olga Chertkov Lynne A Goodwin Cameron R Currie David Mead Phillip J Brumm |
| author_facet | Garret Suen Paul J Weimer David M Stevenson Frank O Aylward Julie Boyum Jan Deneke Colleen Drinkwater Natalia N Ivanova Natalia Mikhailova Olga Chertkov Lynne A Goodwin Cameron R Currie David Mead Phillip J Brumm |
| author_sort | Garret Suen |
| collection | DOAJ |
| description | Fibrobacter succinogenes is an important member of the rumen microbial community that converts plant biomass into nutrients usable by its host. This bacterium, which is also one of only two cultivated species in its phylum, is an efficient and prolific degrader of cellulose. Specifically, it has a particularly high activity against crystalline cellulose that requires close physical contact with this substrate. However, unlike other known cellulolytic microbes, it does not degrade cellulose using a cellulosome or by producing high extracellular titers of cellulase enzymes. To better understand the biology of F. succinogenes, we sequenced the genome of the type strain S85 to completion. A total of 3,085 open reading frames were predicted from its 3.84 Mbp genome. Analysis of sequences predicted to encode for carbohydrate-degrading enzymes revealed an unusually high number of genes that were classified into 49 different families of glycoside hydrolases, carbohydrate binding modules (CBMs), carbohydrate esterases, and polysaccharide lyases. Of the 31 identified cellulases, none contain CBMs in families 1, 2, and 3, typically associated with crystalline cellulose degradation. Polysaccharide hydrolysis and utilization assays showed that F. succinogenes was able to hydrolyze a number of polysaccharides, but could only utilize the hydrolytic products of cellulose. This suggests that F. succinogenes uses its array of hemicellulose-degrading enzymes to remove hemicelluloses to gain access to cellulose. This is reflected in its genome, as F. succinogenes lacks many of the genes necessary to transport and metabolize the hydrolytic products of non-cellulose polysaccharides. The F. succinogenes genome reveals a bacterium that specializes in cellulose as its sole energy source, and provides insight into a novel strategy for cellulose degradation. |
| first_indexed | 2024-04-12T07:17:58Z |
| format | Article |
| id | doaj.art-14a5ec0919d84973a80ed1cad43d5998 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T07:17:58Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-14a5ec0919d84973a80ed1cad43d59982022-12-22T03:42:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0164e1881410.1371/journal.pone.0018814The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist.Garret SuenPaul J WeimerDavid M StevensonFrank O AylwardJulie BoyumJan DenekeColleen DrinkwaterNatalia N IvanovaNatalia MikhailovaOlga ChertkovLynne A GoodwinCameron R CurrieDavid MeadPhillip J BrummFibrobacter succinogenes is an important member of the rumen microbial community that converts plant biomass into nutrients usable by its host. This bacterium, which is also one of only two cultivated species in its phylum, is an efficient and prolific degrader of cellulose. Specifically, it has a particularly high activity against crystalline cellulose that requires close physical contact with this substrate. However, unlike other known cellulolytic microbes, it does not degrade cellulose using a cellulosome or by producing high extracellular titers of cellulase enzymes. To better understand the biology of F. succinogenes, we sequenced the genome of the type strain S85 to completion. A total of 3,085 open reading frames were predicted from its 3.84 Mbp genome. Analysis of sequences predicted to encode for carbohydrate-degrading enzymes revealed an unusually high number of genes that were classified into 49 different families of glycoside hydrolases, carbohydrate binding modules (CBMs), carbohydrate esterases, and polysaccharide lyases. Of the 31 identified cellulases, none contain CBMs in families 1, 2, and 3, typically associated with crystalline cellulose degradation. Polysaccharide hydrolysis and utilization assays showed that F. succinogenes was able to hydrolyze a number of polysaccharides, but could only utilize the hydrolytic products of cellulose. This suggests that F. succinogenes uses its array of hemicellulose-degrading enzymes to remove hemicelluloses to gain access to cellulose. This is reflected in its genome, as F. succinogenes lacks many of the genes necessary to transport and metabolize the hydrolytic products of non-cellulose polysaccharides. The F. succinogenes genome reveals a bacterium that specializes in cellulose as its sole energy source, and provides insight into a novel strategy for cellulose degradation.http://europepmc.org/articles/PMC3079729?pdf=render |
| spellingShingle | Garret Suen Paul J Weimer David M Stevenson Frank O Aylward Julie Boyum Jan Deneke Colleen Drinkwater Natalia N Ivanova Natalia Mikhailova Olga Chertkov Lynne A Goodwin Cameron R Currie David Mead Phillip J Brumm The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. PLoS ONE |
| title | The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. |
| title_full | The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. |
| title_fullStr | The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. |
| title_full_unstemmed | The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. |
| title_short | The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. |
| title_sort | complete genome sequence of fibrobacter succinogenes s85 reveals a cellulolytic and metabolic specialist |
| url | http://europepmc.org/articles/PMC3079729?pdf=render |
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